The Nature of Intergeneric Episomal Infection

Use of normal probability paper in determining thermal melting values of deoxyribonucleic acid

“…DNA from microorganisms containing satellite bands in density gradient centrifugation, indicating DNA with a different base composition, has been described by Falkow et al (9), Suyama and Gibson (24), and Moustacchi and Williamson (20). DNA satellite bands have also been observed in Serratia and Proteus, both of which contain episomes (17,21). Recently, Jyssum and Jyssum (12) suggested that loss of competence in Neisseria nzeningitidis was accompanied by loss of chromosomal integration of an episomal competence factor.…”

Hydrodynamic and Optical Rotatory Dispersion Studies on Wheat Germ Soluble Ribonucleic Acid*

“…In order to evaluate the forces responsible for maintaining the secondary structure of wheat germ s-RNA, ethylene glycol, a solvent which has been shown to preferentially break hydrophobic bonds in some proteins (Sage and Singer, 1962; Kay and Brahms, 1963; Green and , in DNA (Marmur et al, 1963), and in poly-C and yeast s-RNA (Fasman et al, 1964(Fasman et al, , 1965, was employed. A collapse of the native s R N A structure was effected by this reagent, suggesting that hydrophobic forces are playing a significant role in the stabilization of its native conformation.…”

Genetic Manipulations with Plant Material

“…Therefore, the reaction rate depends on their concentration. The renaturation kinetics is a secondorder process (Marmur and Doty, 1961J Marmur et al, 1963J Huebner and Todaro, 1969, and the rate-determining step appears to be the initial nucleation reaction rather than the subsequent "zippering" process. The reaction rate is given by the equation : dC = _ kC 2 dt in which C is the single-stranded DNA concentration at any time t, and k the second-order rate constant.…”