The nanostructured secretome

Busatto, Sara; Zendrini, Andrea; Radeghieri, Annalisa; Paolini, Lucia; Romano, Miriam; Presta, Marco; Bergese, Paolo

doi:10.1039/c9bm01007f

Cited by 45 publications

(47 citation statements)

References 228 publications

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“…Compositional properties have been studied extensively; however, comparatively little attention has been given to nano-mechanical features until now. Therefore, this review only covers the aspect of compositional characteristics of EVs as a therapeutic carrier [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Therapeutic miRNA-Enriched Extracellular Vesicles: Current Approaches and Future Prospects

Munir

Yoon

Ryu

2020

Cells

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Extracellular vesicles (EVs) are 50–300 nm vesicles secreted by eukaryotic cells. They can carry cargo (including miRNA) from the donor cell to the recipient cell. miRNAs in EVs can change the translational profile of the recipient cell and modulate cellular morphology. This endogenous mechanism has attracted the attention of the drug-delivery community in the last few years. EVs can be enriched with exogenous therapeutic miRNAs and used for treatment of diseases by targeting pathological recipient cells. However, there are some obstacles that need to be addressed before introducing therapeutic miRNA-enriched EVs in clinics. Here, we focused on the progress in the field of therapeutic miRNA enriched EVs, highlighted important areas where research is needed, and discussed the potential to use them as therapeutic miRNA carriers in the future.

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Section: Introductionmentioning

confidence: 99%

Therapeutic miRNA-Enriched Extracellular Vesicles: Current Approaches and Future Prospects

Munir

Yoon

Ryu

2020

Cells

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“…Almost all cell types secrete nano-and micro-sized vesicles used for intercellular communication (Raposo and Stahl 2019). Granting control over the production and composition of those vesicles hold great promises in nanotechnology and nanomedicine (Busatto et al 2020). In this regard, the protein-induced intracellular membrane proliferation could increase the vesicle production yield.…”

Section: Membrane Overproduction As a Platform For Biotechnological Amentioning

confidence: 99%

Intracellular Membranes: Conserved Mechanisms of Formation and Regulation Throughout Evolution

Mir¹,

Biou²,

Dautin³

et al. 2020

Preprint

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Membrane remodeling and phospholipid biosynthesis are normally tightly regulated to maintain the shape and function of cells. Indeed, different physiological mechanisms ensure a precise coordination between de novo phospholipid biosynthesis and modulation of membrane morphology. Interestingly, the overproduction of certain membrane proteins hijack these regulation networks, leading to the formation of impressive intracellular membrane structures in both prokaryotic and eukaryotic cells. The proteins triggering membrane proliferation share two major common features: 1) they promote the formation of highly curved membrane domains and 2) they lead to an enrichment in anionic, cone-shaped phospholipids (cardiolipin or phosphatidic acid) in the newly formed membranes. Taking into account the available examples of membrane proliferation upon protein overproduction, together with the latest biochemical, biophysical and structural data, we explore the relationship between protein synthesis and membrane biogenesis. We propose a mechanism for the formation of these non-physiological intracellular membranes that shares similarities with natural inner membrane structures found in α-proteobacteria, mitochondria and some viruses-infected cells, pointing towards a conserved feature through evolution. We hope that the information discussed in this review will give a better grasp of the biophysical mechanisms behind physiological and induced intracellular membrane proliferation, inspiring new biotechnological applications.

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“…EVs derived from mesenchymal stromal/stem cells (MSCs) display similar functions as their parental cells and show therapeutic efficacy in many non-clinical models (Keshtkar et al, 2018). MSC-derived EVs (MSC-EVs) exert their functions through the transfer of their cargo (i.e., proteins, lipids, and nucleic acids such as mRNA, micro-RNAs, long non-coding RNAs, DNA, and metabolites) (Busatto et al, 2019;Qiu et al, 2019). They can be used as therapeutic tools either in naïve form, as substitutes of intact cells, or after modification for targeted drug delivery.…”

Section: Introductionmentioning

confidence: 99%

Mesenchymal Stem Cell-Derived Extracellular Vesicles: Opportunities and Challenges for Clinical Translation

Maumus

Rozier

Boulestreau

et al. 2020

Front. Bioeng. Biotechnol.

105

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Extracellular vesicles (EVs), including exosomes and microvesicles, derived from mesenchymal stem/stromal cells (MSCs) exert similar effects as their parental cells, and are of interest for various therapeutic applications. EVs can act through uptake by the target cells followed by release of their cargo inside the cytoplasm, or through interaction of membrane-bound ligands with receptors expressed on target cells to stimulate downstream intracellular pathways. EV-based therapeutics may be directly used as substitutes of intact cells or after modification for targeted drug delivery. However, for the development of EV-based therapeutics, several production, isolation, and characterization requirements have to be met and the quality of the final product has to be tested before its clinical implementation. In this review, we discuss the challenges associated with the development of EV-based therapeutics and the regulatory specifications for their successful clinical translation.

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The nanostructured secretome

Abstract: The term secretome should be expanded to also include the great variety of nanoparticles secreted by cells into the extracellular space, which ranges from lipoproteins of a few nanometers to extracellular vesicles of hundreds of nanometers.

Cited by 45 publications

References 228 publications

Therapeutic miRNA-Enriched Extracellular Vesicles: Current Approaches and Future Prospects

Therapeutic miRNA-Enriched Extracellular Vesicles: Current Approaches and Future Prospects

Intracellular Membranes: Conserved Mechanisms of Formation and Regulation Throughout Evolution

Mesenchymal Stem Cell-Derived Extracellular Vesicles: Opportunities and Challenges for Clinical Translation

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