The nanoscale geometry of TiO2 nanotubes influences the osteogenic differentiation of human adipose-derived stem cells by modulating H3K4 trimethylation

Lv, Longwei; Liu, Yunsong; Zhang, Ping; Zhang, Xiao; Liu, Jianzhang; Chen, Tong; Su, Penglei; Li, Hongyi; Zhou, Yongsheng

doi:10.1016/j.biomaterials.2014.11.002

Cited by 175 publications

(123 citation statements)

References 46 publications

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“…27 45 Furthermore, we found that RBP2 expression was down-regulated in hASCs in contact with TiO 2 nanotubes. 46 We therefore asked whether single-layer graphene would also influence the behavior of MSCs through epigenetic regulation?…”

Section: Epigenetic Mechanism Of Graphene On Osteogenic Differentiatimentioning

confidence: 99%

Single-Layer Graphene Enhances the Osteogenic Differentiation of Human Mesenchymal Stem Cells In Vitro and In Vivo

Liu¹,

Chen²,

Du³

et al. 2016

j biomed nanotechnol

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In recent years, although several studies have demonstrated the potential of graphene-coated substrates in promoting attachment, proliferation and differentiation of osteoblasts and mesenchymal stem cells (MSCs), the effects of single-layer graphene on the osteogenic differentiation of human MSCs (hMSCs) remains unclear, especially in vivo. In this study, we transferred chemical vapor deposition (CVD) grown single-layer graphene to glass slides and observed its effects on adhesion, proliferation and osteogenic differentiation of human adipose-derived stem cells (hASCs) and human bone marrow mesenchymal stem cells (hBMMSCs) in vitro. Then, in vivo, we incubated hASCs and hBMMSCs on single-layer graphene-coated smooth titanium (Ti) disks before implanting them into the back subcutaneous area of nude mice. We found that single-layer graphene accelerated cell adhesion to the substrate without influencing cell proliferation of hMSCs. Moreover, we present the first study that explores the epigenetic role of single-layer graphene in determining stem cell fate. By utilizing epigenetic approaches, we reveal that single-layer graphene promotes osteogenic differentiation of hMSCs both in vitro and in vivo, potentially by upregulating methylation of H3K4 at the promoter regions of osteogenesis-associated genes. Overall, our results highlight the potential of this material in implants and injured tissues in clinical applications.

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Section: Epigenetic Mechanism Of Graphene On Osteogenic Differentiatimentioning

confidence: 99%

Single-Layer Graphene Enhances the Osteogenic Differentiation of Human Mesenchymal Stem Cells In Vitro and In Vivo

Liu¹,

Chen²,

Du³

et al. 2016

j biomed nanotechnol

Self Cite

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“…[13][14][15] In the past decade, design and fabrication of nanostructured titania and titanate layers on titanium have attracted much attention because of the improved biological performance of titanium and its widespread application in bone implants. [14][15][16][17][18] Generally,…”

Section: Introductionmentioning

confidence: 99%

Tuning the surface microstructure of titanate coatings on titanium implants for enhancing bioactivity of implants

Wang

Lai

Zheng

et al. 2015

IJN

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Biological performance of artificial implant materials is closely related to their surface characteristics, such as microtopography, and composition. Therefore, convenient fabrication of artificial implant materials with a cell-friendly surface structure and suitable composition was of great significance for current tissue engineering. In this work, titanate materials with a nanotubular structure were successfully fabricated through a simple chemical treatment. Immersion test in a simulated body fluid and in vitro cell culture were used to evaluate the biological performance of the treated samples. The results demonstrate that the titanate layer with a nanotubular structure on Ti substrates can promote the apatite-inducing ability remarkably and greatly enhance cellular responses. This highlights the potential of such titanate biomaterials with the special nanoscale structure and effective surface composition for biomedical applications such as bone implants.

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“…It was also shown that TiO 2 nanotubes of 70 nm in diameter were the optimal dimension for the osteogenic differentiation of human adipose-derived stem cells. They were also shown to stimulate methylation of histone H3 at lysine 4 (H3K4) in the promoter of Runx2 and osteocalcin 44 . These results indicate that there is an optimal nanotopological diameter, which facilitates transcriptional activation for osteogenic differentiation.…”

Section: Discussionmentioning

confidence: 99%

Nanotopological plate stimulates osteogenic differentiation through TAZ activation

Hwang

Lee

Byun

et al. 2017

Sci Rep

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The topographical environment, which mimics the stem cell niche, provides mechanical cues to regulate the differentiation of mesenchymal stem cells (MSC). Diverse topographical variations have been engineered to investigate cellular responses; however, the types of mechanical parameters that affect cells, and their underlying mechanisms remain largely unknown. In this study, we screened nanotopological pillars with size gradient to activate transcriptional coactivator with PDZ binding motif (TAZ), which stimulates osteogenesis of MSC. We observed that a nanotopological plate, 70 nm in diameter, significantly induces osteogenic differentiation with the activation of TAZ. TAZ activation via the nanotopological plate was mediated by actin polymerization and Rho signaling, as evidenced by the cytosolic localization of TAZ under F-actin or Rho kinase inhibitor. The FAK and MAPK pathways also play a role in TAZ activation by the nanotopological plate because the inhibitor of ERK and JNK blocked nanopattern plate induced osteogenic differentiation. Taken together, these results indicate that nanotopology regulates cell differentiation through TAZ activation.

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The nanoscale geometry of TiO2 nanotubes influences the osteogenic differentiation of human adipose-derived stem cells by modulating H3K4 trimethylation

Cited by 175 publications

References 46 publications

Single-Layer Graphene Enhances the Osteogenic Differentiation of Human Mesenchymal Stem Cells In Vitro and In Vivo

Single-Layer Graphene Enhances the Osteogenic Differentiation of Human Mesenchymal Stem Cells In Vitro and In Vivo

Tuning the surface microstructure of titanate coatings on titanium implants for enhancing bioactivity of implants

Nanotopological plate stimulates osteogenic differentiation through TAZ activation

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