The Na,K-ATPase Receptor Complex: Its Organization and Membership

Pierre, Sandrine V.; Xie, Zijian

doi:10.1385/cbb:46:3:303

Cited by 136 publications

(118 citation statements)

References 100 publications

Supporting

Mentioning

113

Contrasting

Unclassified

Order By: Relevance

“…The importance and the very existence of such factors has been a matter of controversy; 10, 11 however, notable breakthroughs in the field include the identification of specific endogenous cardiotonic steroids in experimental and clinical studies, 12-15 the detection of altered concentrations of endogenous cardiotonic steroids in different disease states, the elucidation of the roles of endogenous cardiotonic steroids in these settings, 16,17 and, in parallel, the discovery of the signaling functions of the Na + /K + -ATPase and its involvement in many elements of basic cell biology. 18,19 These issues, particularly the last, have been reviewed in a series of excellent papers. [16][17][18][19][20][21][22][23] The main purpose of the present Review is to emphasize the roles of endogenous cardiotonic steroids in human health and disease-in particular with regard to the renal and cardiovascular systems-and to highlight potential therapeutic targets that have arisen as a result of research in this area.…”

Section: Introductionmentioning

confidence: 99%

“…18,19 These issues, particularly the last, have been reviewed in a series of excellent papers. [16][17][18][19][20][21][22][23] The main purpose of the present Review is to emphasize the roles of endogenous cardiotonic steroids in human health and disease-in particular with regard to the renal and cardiovascular systems-and to highlight potential therapeutic targets that have arisen as a result of research in this area. In short, we hope to establish that endogenous cardiotonic steroids have begun their journey from bench to bedside.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Endogenous digitalis: pathophysiologic roles and therapeutic applications

2008

View full text Add to dashboard Cite

SUMMARYEndogenous digitalis-like factors, also called cardiotonic steroids, have been thought for nearly half a century to have important roles in health and disease. The endogenous cardiotonic steroids ouabain and marinobufagenin have been identified in humans, and an effector mechanism has been delineated by which these hormones signal through the sodium/potassium-transporting ATPase. These findings have increased interest in this field substantially. Although cardiotonic steroids were first considered important in the regulation of renal sodium transport and arterial pressure, subsequent work has implicated these hormones in the control of cell growth, apoptosis and fibrosis, among other processes. This Review focuses on the role of endogenous cardiotonic steroids in the pathophysiology of essential hypertension, congestive heart failure, end-stage renal disease and pre-eclampsia. We also discuss potential therapeutic strategies that have emerged as a result of the increased understanding of the regulation and actions of cardiotonic steroids.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Endogenous digitalis: pathophysiologic roles and therapeutic applications

2008

View full text Add to dashboard Cite

show abstract

“…Further, by producing a transmembrane Na ϩ gradient, this pump drives vectorial transport of a number of solutes across epithelia. Finally, it plays a role in intracellular signaling by a mechanism which is independent of ion transport, but similar to ion transport by this pump is sensitive to ouabain (17).…”

mentioning

confidence: 99%

Inverse Correlation between the Extent of N-Glycan Branching and Intercellular Adhesion in Epithelia

Vagin

Tokhtaeva

Yakubov

et al. 2008

Journal of Biological Chemistry

View full text Add to dashboard Cite

The majority of cell adhesion molecules are N-glycosylated, but the role of N-glycans in intercellular adhesion in epithelia remains ill-defined. Reducing N-glycan branching of cellular glycoproteins by swainsonine, the inhibitor of N-glycan processing, tightens and stabilizes cell-cell junctions as detected by a 3-fold decrease in the paracellular permeability and a 2-3-fold increase in the resistance of the adherens junction proteins to extraction by non-ionic detergent. In addition, exposure of cells to swainsonine inhibits motility of MDCK cells. Mutagenic removal of N-glycosylation sites from the Na,K-ATPase ␤ 1 subunit impairs cell-cell adhesion and decreases the effect of swainsonine on the paracellular permeability of the cell monolayer and also on detergent resistance of adherens junction proteins, indicating that the extent of N-glycan branching of this subunit is important for intercellular adhesion. The N-glycans of the Na,K-ATPase ␤ 1 subunit and E-cadherin are less complex in tight renal epithelia than in the leakier intestinal epithelium. The complexity of the N-glycans linked to these proteins gradually decreases upon the formation of a tight monolayer from dispersed MDCK cells. This correlates with a cell-cell adhesion-induced increase in expression of GnT-III (stops N-glycan branching) and a decrease in expression of GnTs IVC and V (promote N-glycan branching) as detected by realtime quantitative PCR. Consistent with these results, partial silencing of the gene encoding GnT-III increases branching of N-glycans linked to the Na,K-ATPase ␤ 1 subunit and other glycoproteins and results in a 2-fold increase in the paracellular permeability of MDCK cell monolayers. These results suggest epithelial cells can regulate tightness of cell junctions via remodeling of N-glycans, including those linked to the Na,K-ATPase ␤ 1 -subunit.

show abstract

“…Studies during the last 10 years, mainly from our group 50 and from the group of Zian Xie, 51 have shed new light on the function of the endogenous CTSs. These studies have revealed that Na + ,K + -ATPase is also a signal transducer and that its signaling function is activated by ouabain.…”

mentioning

confidence: 99%

2011 Homer Smith Award

Aperia

2012

Journal of the American Society of Nephrology

View full text Add to dashboard Cite

The Na,K-ATPase Receptor Complex: Its Organization and Membership

Cited by 136 publications

References 100 publications

Endogenous digitalis: pathophysiologic roles and therapeutic applications

Endogenous digitalis: pathophysiologic roles and therapeutic applications

Inverse Correlation between the Extent of N-Glycan Branching and Intercellular Adhesion in Epithelia

2011 Homer Smith Award

Contact Info

Product

Resources

About