The myocardin-related transcription factor, MASTR, cooperates with MyoD to activate skeletal muscle gene expression

Meadows, Stryder M.; Warkman, Andrew S.; Salanga, Matthew C.; Small, Eric M.; Krieg, Paul A.

doi:10.1073/pnas.0703918105

Cited by 23 publications

(23 citation statements)

References 38 publications

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“…8A, lanes 1-3). Myocardin activates SM-MHC but also SM22␣ and calponin as previously shown (Small et al, 2005;Meadows et al, 2008) (Fig. 8B, lane 3).…”

Section: Induction Of Smooth Muscle Gene Expression By Transcription supporting

confidence: 80%

Induction and modulation of smooth muscle differentiation in Xenopus embryonic cells

Barillot

Tréguer

Faucheux

et al. 2008

Developmental Dynamics

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By comparison with skeletal or cardiac developmental programs, little is known regarding the specific factors that promote specification and differentiation of smooth muscle cells from pluripotent cells. We have analyzed the developmental expression of a subset of smooth muscle genes during Xenopus early development and showed that similar to mammals and avians, Xenopus smooth muscle myosin heavy chain (SM-MHC) is a highly specific marker of smooth muscle differentiation. Embryonic cells from animal pole explants of Xenopus blastula can be induced by basic fibroblast growth factor, Wnt, and bone morphogenetic protein signals to adopt the smooth muscle pathway. Explants from early embryos that contain neural crest cells can also differentiate into cells expressing smooth muscle genes. We examined the interplay of several transcription factors, that is SRF, myocardin, and GATA6, that induce the expression of SM-MHC in animal cap cells and found that myocardin-dependent expression of smooth muscle genes in animal cap cells is synergized by SRF but is strongly antagonized by GATA6. Developmental Dynamics 237: 3373-3386, 2008.

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“…8A, lanes 1-3). Myocardin activates SM-MHC but also SM22␣ and calponin as previously shown (Small et al, 2005;Meadows et al, 2008) (Fig. 8B, lane 3).…”

Section: Induction Of Smooth Muscle Gene Expression By Transcription supporting

confidence: 80%

Induction and modulation of smooth muscle differentiation in Xenopus embryonic cells

Barillot

Tréguer

Faucheux

et al. 2008

Developmental Dynamics

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“…The absence of MRTF-A and -B in skeletal muscle results in perinatal death accompanied by small myofibers and incomplete sarcomere formation. These results highlight the importance of the MRTF/ SRF pathway as a central regulator of several muscle-specific genes Garg et al, 2014;Kuwahara et al, 2007;Meadows et al, 2008).…”

Section: Discussionmentioning

confidence: 82%

“…In the case of the muscle-specific protein LMOD3, MRTF regulates actin turnover by binding actin directly . Other MRTF targets, such as MASTR, contribute to myogenesis by recruiting myogenic transcription factors, such as MyoD (Meadows et al, 2008). ) and MRTF dKO mice at E17.5.…”

Section: Discussionmentioning

confidence: 99%

“…Another member of the MRTF family, MEF2 activating motif and SAP domain containing transcriptional regulator (MASTR), cooperates with MyoD (also known as MYOD1) to turn on the myogenic program during skeletal muscle regeneration (Creemers et al, 2006;Meadows et al, 2008). MASTR and MRTF-A are required for skeletal muscle regeneration in adult mice.…”

Section: Introductionmentioning

confidence: 99%

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Myocardin-related transcription factors are required for skeletal muscle development

et al. 2016

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Myocardin-related transcription factors (MRTFs) play a central role in the regulation of actin expression and cytoskeletal dynamics. Stimuli that promote actin polymerization allow for shuttling of MRTFs to the nucleus where they activate serum response factor (SRF), a regulator of actin and other cytoskeletal protein genes. SRF is an essential regulator of skeletal muscle differentiation and numerous components of the muscle sarcomere, but the potential involvement of MRTFs in skeletal muscle development has not been examined. We explored the role of MRTFs in muscle development in vivo by generating mutant mice harboring a skeletal muscle-specific deletion of MRTF-B and a global deletion of MRTF-A. These double knockout (dKO) mice were able to form sarcomeres during embryogenesis. However, the sarcomeres were abnormally small and disorganized, causing skeletal muscle hypoplasia and perinatal lethality. Transcriptome analysis demonstrated dramatic dysregulation of actin genes in MRTF dKO mice, highlighting the importance of MRTFs in actin cycling and myofibrillogenesis. MRTFs were also shown to be necessary for the survival of skeletal myoblasts and for the efficient formation of intact myotubes. Our findings reveal a central role for MRTFs in sarcomere formation during skeletal muscle development and point to the potential involvement of these transcriptional co-activators in skeletal myopathies.

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“…In stage 33-34 embryos, the presence of kidins220 mRNA can be observed in forebrain, midbrain, and hindbrain, eye vesicle, branchial arches and somite region, (Fig.1 E,F), as highlighted using mastr gene, a typical somitic marker ( Fig. 1G) (Meadows et al, 2008). kidins220 expression is detected in heart region and in otic vesicle at stage 35-36 (Fig.…”

Section: Resultsmentioning

confidence: 97%

Kidins220/ARMS is dynamically expressed during Xenopus laevis development

Marracci

Giannini²,

Vitiello³

et al. 2013

Int. J. Dev. Biol.

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Kidins220 (Kinase D interacting substrate of 220 kDa)/ARMS (Ankyrin Repeat-rich Membrane Spanning) is a conserved scaffold protein that acts as a downstream substrate for protein kinase D and mediates multiple receptor signalling pathways. Despite the dissecting of the function of this protein in mammals, using both in vitro and in vivo studies, a detailed characterization of its gene expression during early phases of embryogenesis has not been described yet. Here, we have used Xenopus laevis as a vertebrate model system to analyze the gene expression and the protein localization of Kidins220/ARMS. We found its expression was dynamically regulated during development. Kidins220/ARMS mRNA was expressed from neurula to larval stage in different embryonic regions including the nervous system, eye, branchial arches, heart and somites. Similar to the transcript, the protein was present in multiple embryonic domains including the central nervous system, cranial nerves, motor nerves, intersomitic junctions, retinal ganglion cells, lens, otic vesicle, heart and branchial arches. In particular, in some regions such as the retina and somites, the protein displayed a differential localization pattern in stage 42 embryos when compared to the earlier examined stages. Taken together our results suggest that this multidomain protein is involved in distinct spatio-temporal differentiative events.

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The myocardin-related transcription factor, MASTR, cooperates with MyoD to activate skeletal muscle gene expression

Abstract: Myf5 ͉ serum response factor ͉ Xenopus

Cited by 23 publications

References 38 publications

Induction and modulation of smooth muscle differentiation in Xenopus embryonic cells

Induction and modulation of smooth muscle differentiation in Xenopus embryonic cells

Myocardin-related transcription factors are required for skeletal muscle development

Kidins220/ARMS is dynamically expressed during Xenopus laevis development

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