The mycobacteria: an introduction to nomenclature and pathogenesis

Rastogi, Nalin; Legrand, Eric; Sola, Christophe

doi:10.20506/rst.20.1.1265

Cited by 162 publications

(107 citation statements)

References 163 publications

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“…In clinical mycobacteriology laboratories, identification of mycobacterial cultures is usually carried out by means of biochemical methods [5]. However, apart from the fact that the methodology is time consuming, it is sometimes difficult to discriminate between closely related taxa on the basis of phenotypic characteristics.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a Commercial Line Probe Assay for Identification of Mycobacterium Species from Liquid and Solid Culture

Mijs

Vreese

Devos

et al. 2002

European Journal of Clinical Microbiology & Infectious Dise

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The performance of a commercial line probe assay (LiPA) (Inno-LiPA Mycobacteria; Innogenetics, Belgium) for the detection and identification of Mycobacterium species from liquid and solid culture was evaluated at five routine clinical laboratories. The LiPA method is based on the reverse hybridization principle, in which the mycobacterial 16S-23S ribosomal RNA (rRNA) spacer region is amplified by polymerase chain reaction (PCR). Amplicons are subsequently hybridized with oligonucleotide probes arranged on a membrane strip and detected by a colorimetric system. The test detects the presence of Mycobacterium species and specifically identifies Mycobacterium tuberculosis complex, Mycobacterium kansasii, Mycobacterium xenopi, Mycobacterium gordonae, Mycobacterium avium complex, Mycobacterium avium, Mycobacterium intracellulare, Mycobacterium scrofulaceum, and Mycobacterium chelonae - Mycobacterium abscessus complex. The results of LiPA were compared with the results obtained using traditional biochemical and molecular tests (DNA probe-based techniques, PCR restriction enzyme analysis of the 65 kDa heat-shock protein gene, and sequencing of the 16S rDNA). A total of 669 isolates, 642 of which were identified as Mycobacterium species and 27 as non- Mycobacterium species, were tested by LiPA. After analysis of 14 initially discordant results and exclusion of one isolate, concordant results were obtained for 636 of 641 Mycobacterium isolates (99.2% accuracy). All Mycobacterium species reacted with the MYC ( Mycobacterium species) probe (100% sensitivity), and all non- Mycobacterium species were identified as such (100% specificity).

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Section: Introductionmentioning

confidence: 99%

Evaluation of a Commercial Line Probe Assay for Identification of Mycobacterium Species from Liquid and Solid Culture

Mijs

Vreese

Devos

et al. 2002

European Journal of Clinical Microbiology & Infectious Dise

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“…Due to its action on the mycobacterial wall, this active substance would be of great interest. Indeed, it has been established that this wall play a fundamental role in the adaptation of mycobacteria to intracellular growth and survival, and in the immunizing modulation and resistance to drugs (Rastogi et al, 2001). Further researches are in progress to improve the use of this active substance in extraction of DNA from different mycobacteria.…”

Section: Extraction Of Mycobacterial Dna By the Staphylococcal Strainmentioning

confidence: 99%

Isolation and identification of a staphylococal strain with an anti-mycobacterial activity and study of it’s mode of action

et al. 2007

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The resistance of mycobacteria to the clinical applied antibiotics poses a serious problem to deal with the infections they cause. So, the search for new antibiotics active against these bacteria becomes urgent. We report here the isolation from a Moroccan biotope of a bacterial strain secreting an active substance of protein nature that inhibits the growth of several mycobacterial species (Mycobacterium smegmatis; M. aurum A+; M. vaccae; M. bovis BCG and M. kansasii). PCR amplification and DNA sequencing of the 16S ribosomal RNA gene allowed the identification of this strain as Staphylococcus haemolyticus. Moreover, the substance produced by this strain was able to lyse the wall of M. smegmatis and to extract its genomic DNA indicating that it acts probably, like others anti-mycobacterial antibiotics, on this envelope. The identification and characterisation of the active substance would open the way for further technological and therapeutic investigations.

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“…Classified in the family Mycobacteriaceae, M leprae is a rod-shaped organism with parallel sides and rounded ends, 1 to 8 m long and 0.3 m in diameter. 92 It is an obligatory intracellular parasite that divides by means of binary fission. Macrophages are the main target of infection, where the bacteria occur in clumps (''globi'') with hundreds of individuals.…”

Section: Leprosymentioning

confidence: 99%

“…It is a gram-positive bacterium that is also strongly acid-fast after staining with carbol-fuchsin. 92 In the footpads of mice, M leprae has a generation time of 11 to 13 days, extremely slow even for slowgrowing mycobacteria (M tuberculosis having a generation time of 20 hours). 92 This slow rate of multiplication is consistent, however, with the chronicity of the disease.…”

Section: Leprosymentioning

confidence: 99%