The murine sublingual and submandibular mucins their isolation and characterization

Roukema, P.A.; Oderkerk, C.H.; Salkinoja‐Salonen, Mirja

doi:10.1016/0304-4165(76)90051-9

Cited by 53 publications

(17 citation statements)

References 22 publications

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“…The major mucins of adult sublingual glands of rats and mice have been isolated and partially characterized. Based on size, amino acid and carbohydrate composition, and antigenicity, investigators have concluded that the sublingual gland mucin is very different from the mucin found in the seromucous acinar cells of the adult rodent submandibular gland (Moschera and Pigman, 1975;Roukema et al, 1976;Denny and Denny, 1984;Tabak et al, 1985;Culp et al, 1991;Man et al, 1995). Recent comparison of cDNA sequences of mucin transcripts from mouse submandibular and sublingual glands revealed very little homology between the two and confirmed their separate genetic origins (Denny and Denny, unpublished).…”

Section: (2) Perinatal and Adult Protein Phenotypesmentioning

confidence: 99%

Salivary Glands: A Paradigm for Diversity of Gland Development

Denny

Ball

Redman

1997

Critical Reviews in Oral Biology & Medicine

177

171

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The major salivary glands of mammals are represented by three pairs of organs that cooperate functionally to produce saliva for the oral cavity. While each type of gland produces a signature secretion that complements the secretions from the other glands, there is also redundancy as evidenced by secretion of functionally similar and, in some cases, identical products in the three glands. This, along with their common late initiation of development, in fetal terms, their similarities in developmental pattern, and their proximate sites of origin, suggests that a common regulatory cascade may have been shared until shortly before the onset of overt gland development. Furthermore, occasional ectopic differentiation of individual mature secretory cells in the wrong" gland suggests that control mechanisms responsible for the distinctive cellular composition of each gland also share many common steps, with only minor differences providing the impetus for diversification. To begin to address this area, we examine here the origins of the salivary glands by reviewing the expression patterns of several genes with known morphogenetic potential that may be involved based on developmental timing and location. The possibility that factors leading to determination of the sites of mammalian salivary gland development might be homologous to the regulatory cascade leading to salivarv gland formation in Drosophila is also evaluated. In a subsequent section, cellular phenotypes of neonatal and adult glands are compared and evaluated for insights into the mechanisms and lineages leading to cellular diversification. Finally, the phenomena of proliferation, repair, and regeneration in adult salivary glands are reviewed, with emphasis on the extent to which the cellular diversity is reversible and which cell type other than stem cells has the ability to redifferentiate into other cell types.

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Section: (2) Perinatal and Adult Protein Phenotypesmentioning

confidence: 99%

Salivary Glands: A Paradigm for Diversity of Gland Development

Denny

Ball

Redman

1997

Critical Reviews in Oral Biology & Medicine

177

171

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“…We have isolated salivary mucins from human mucous extraparotid saliva and whole saliva by an ultracentrifugation procedure described by Roukema et al [1976]. In this paper we report the influ ence of human salivary mucins on the de mineralization of human dental enamel by citric acid.…”

mentioning

confidence: 98%

“…About 16 mg dry weight of HWSM (range 12-28 mg) could be isolated from 100 ml CHWS using the ultracentrifuga tion method described by Roukema et al [1976Roukema et al [ , 1982. This is in the same order as reported by Fox et al [1985], who found the average amount of HWSM to be 20 m g /100 ml (range 3-51), suggesting that HWSM comprises the major part of the salivary mucins.…”

Section: Human Whole Saliva As a Source O F Mucinsmentioning

confidence: 99%

Role of Mucins from Human Whole Saliva in the Protection of Tooth Enamel against Demineralization in vitro

Amerongen

Oderkerk

Driessen³

1987

Caries Res

Self Cite

121

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The influence of salivary pellicles on the demineralization of human dental enamel by 1% citric acid was studied. The pellicles were formed on embedded human tooth surfaces incubated in vitro with various solutions for periods of up to 6 days. Pellicles induced by unstimulated whole saliva gave an approximately 45% and those from parotid saliva a 25% inhibition of demineralization. On the other hand, a pellicle formed from mixed submandibular and sublingual (SM-SL) saliva in 30 min gave a retardation in lesion formation of at least 40% and after a 60-min exposure to SM-SL saliva, the lesion formation was completely prevented. In contrast, when the mucins were removed from SM-SL saliva by ultracentrifugation, protection against demineralization by the pellicle formed on exposure to the supernatant was only 30%. The effect of pellicles obtained by isolated salivary mucins was also studied. Human whole salivary mucins (HWSM) were isolated from both human SM-SL saliva and whole saliva of two individuals with blood group A. The mucins contained 18% protein, 72% carbohydrate, 1.4% sulfate and 0.14% phosphate. The major components of the protein moiety were threonine (14.7%), valine (12.6%) and serine (10.8%). The molar ratio of the sugar residues was: galactose: N-acetylglucosamine: N-acetylgalactose: fucose: N-acetylneuraminic acid = 6:4.5:4:7:1. A pellicle formed by a 3-day exposure to salivary mucins in vitro gave 100% protection of the tooth surface against demineralization by citric acid. These data suggest that the mucins in human saliva contribute to a large extent to its protective effect against acidic attacks on the tooth surface.

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“…1974;Hand, 1979;Lima and Haddad, 1981]. In particular, N-acetyl-mannosamine is slowly incorporated by the glycoproteins of the sublin gual gland, despite their high content of sialic acid, into which the mannosamine is converted [sialic acid makes up 30% of the saccharide fraction, which contains 66% of the glycoprotein; Moschera and Pigman, 1975;Roukema et al, 1976]. This is said not to be due to a slow cellular uptake [Nieuw Amerongen et al, 1981]; the possibility of a slow conversion to sialic acid seems more likely and is favored by Nieuw Amerongen et al [1981], however, very little is known about the activities of the enzymes catalyzing these reactions.…”

Section: Discussionmentioning

confidence: 99%

“…The results of the present report reveal more specific details about the timetable of the intracellular route that the newly synthesized secretory glycoproteins take through the mucous acinar cells of the major sublingual gland. The analysis of the labeling patterns 0.5, 1 and 2 h after the injection of leucine shows that in the mucous acinar cells the bulk of the protein backbone of the secretory products [90% glycoproteins ;Vreugdenhil and Roukema, 1975;Moschera and Pigman, 1975;Roukema et al, 1976;Nieuw Amerongen et al, 1981] has reached the Golgi apparatus within 2 h, just like many other exo crine cells [see Warshawsky et al, 1963;Caro and Palade, 1964;Jamieson and Palade, 1967a, b;Tiber, 1971;Bogart, 1977], The increased number of silver grains over the apical cell regions at 4 h after the injec tion indicates that it takes at least 4 h for a labeled protein to reach the secretion granules. This means a longer time than in any other exocrine cell investigated until now.…”

Section: Discussionmentioning

confidence: 99%

Radioautographic Study of Glycoprotein Synthesis and Migration in the Major Sublingual Gland of the Mongolian Gerbil (<i>Meriones unguiculatus</i>)

Klenk¹,

Korfsmeier²

1985

Cells Tissues Organs

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Intracellular glycoprotein synthesis and migration in the major sublingual gland of the Mongolian gerbil were studied with light-microscopic radioautography, using L-4, 5-[¾]-leucine, Z)-l-[³H]-glucose and N-[³H]-acetyl-D-mannosamine as precursors. The time elapsed from the beginning of the synthesis of the secretion products to their release was longer in the mucous acinar cells than in any other exocrine cell investigated until now. A slow addition of the terminal sugars (especially sialic acid) to the glycoprotein molecule and a prolonged storage of the glycoproteins in the apical granules were assumed to be the reasons for this delay in schedule. The secretion in the serous demilune cells was much faster than in the mucous acinar cells. The lack of uptake of labelled mannosamine showed that the demilunes are likely to produce substances different from those of the mucous acinar cells, presumably a protein-rich material with a high turnover.

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The murine sublingual and submandibular mucins their isolation and characterization

Cited by 53 publications

References 22 publications

Salivary Glands: A Paradigm for Diversity of Gland Development

Salivary Glands: A Paradigm for Diversity of Gland Development

Role of Mucins from Human Whole Saliva in the Protection of Tooth Enamel against Demineralization in vitro

Radioautographic Study of Glycoprotein Synthesis and Migration in the Major Sublingual Gland of the Mongolian Gerbil (<i>Meriones unguiculatus</i>)

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