The murine homolog of the HIN 200 family, Ifi 204, is constitutively expressed in myeloid cells and selectively induced in the monocyte/macrophage lineage

Gariglio, Marisa; Andrea, Marco De; Lembo, M; Ravotto, M; Zappador, Claudio; Valente, Guido; Landolfo, S

doi:10.1002/jlb.64.5.608

Cited by 38 publications

(43 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This is in contrast to MNDA, which is found in mature granulocytes and monocytes but not in lymphoid cells or undifferentiated myeloid cell lines (11,12). This expression pattern is similar to that of p204, which is constitutively expressed in myelomonocytic cells of the mouse (19).…”

Section: Structure and Expression Of The Hin-200 Proteinsmentioning

confidence: 75%

Transcription and Growth Regulatory Functions of the HIN-200 Family of Proteins

Johnstone

Trapani

1999

Molecular and Cellular Biology

114

125

View full text Add to dashboard Cite

Section: Structure and Expression Of The Hin-200 Proteinsmentioning

confidence: 75%

Transcription and Growth Regulatory Functions of the HIN-200 Family of Proteins

Johnstone

Trapani

1999

Molecular and Cellular Biology

114

125

View full text Add to dashboard Cite

“…(12,13) This growth arrest is accompanied by cell differentiation, as suggested in vitro by the finding that myoblasts overexpressing p204 mature into myotubes (14) and in vivo by the finding that monocytes over-expressing p204 mature into peritoneal macrophages. (15) Finally, our results have demonstrated that expression of a mutant p204 devoid of both Rb-binding motifs interferes with the mouse cytomegalovirus (MCMV) multiplication cycle. The Ifi204 gene is indeed transcriptionally activated by MCMV infection and is required for its replication.…”

Section: Introduction Imentioning

confidence: 87%

Immunohistochemical Expression Analysis of the Human Interferon-Inducible Gene IFI16, a Member of the HIN200 Family, Not Restricted to Hematopoietic Cells

Gariglio¹,

Azzimonti²,

Pagano³

et al. 2002

Journal of Interferon & Cytokine Research

Self Cite

View full text Add to dashboard Cite

This is the first description of an extensive immunohistochemical analysis of interferon (IFN)-inducible gene IFI16 expression in normal tissues. Immunohistochemical detection of IFI16 in paraffin-embedded tissues is achieved by using a polyclonal antibody raised against its C-terminal fragment that recognizes its three closely migrating isoforms in Western blotting. The results clearly indicate that IFI16 expression is not restricted to the hematopoietic compartment. In normal adult human tissues, it is prominent in stratified squamous epithelia and particularly intense in parabasal cells in the proliferating compartments, but it gradually decreases in the more differentiated suprabasal layers. Understanding of IFI16 expression in vivo is essential for interpretation of the results obtained from in vitro studies and elucidation of its physiologic role. The constitutive expression and wider distribution of IFI16 in normal human tissues, not restricted to the hematopoietic compartment, strongly support the possibility of an important role in cell differentiation that can be further modulated by other stimuli, such as IFN. 815

show abstract

“…Interestingly, the IFN-␥ expression level was increased 8.5-fold at 8 h postchallenge in the lungs of RacL11-challenged mice that had been immunized with KyA (Table 3). Since IFN-␥, IFN-␣, and lipopolysaccharide (LPS) induce IFI204 expression (68), it may be significant that IFN-␥ upregulated IFI204 29.7-fold in the lungs of RacL11-challenged mice that had been immunized with KyA 3 days prior to challenge. IFI16, a human homolog of IFI204, has been shown to interact with STING (stimulator of interferon genes), leading to the phosphorylation and nuclear translocation of IRF3 via the IFI16-STING-TBK signaling axis (69,70).…”

Section: Discussionmentioning

confidence: 99%

Immunization with Attenuated Equine Herpesvirus 1 Strain KyA Induces Innate Immune Responses That Protect Mice from Lethal Challenge

Kim

Shakya

O’Callaghan

2016

J Virol

View full text Add to dashboard Cite

Equine herpesvirus 1 (EHV-1) is a major pathogen affecting equines worldwide. The virus causes respiratory disease, abortion, and, in some cases, neurological disease. EHV-1 strain KyA is attenuated in the mouse and equine, whereas wild-type strain RacL11 induces severe inflammation of the lung, causing infected mice to succumb at 4 to 6 days postinfection. Our previous results showed that KyA Equine herpesvirus 1 (EHV-1), a member of the family Herpesviridae and the subfamily Alphaherpesvirinae, is the causative agent of a number of equine pathological states, including severe disease of the central nervous system, respiratory infections, and abortion storms (1-4). Respiratory transmission of this highly contagious virus has resulted in disastrous outbreaks of disease in domestic horse populations and has had significant economic impact on the equine industry. EHV-1 infection of the horse generates a short-lived humoral response but does not confer long-term protection, since disease often occurs following natural infection (5, 6).A model that closely mimics EHV-1 infection in the natural host has been established with various strains of mice (7). Common features include replication in the respiratory mucosae, the development of pneumonitis, cell-associated viremia, and abortion (7,8). Various EHV-1 glycoproteins, including gB, gC, gD, and gH, were capable of inducing neutralizing antibodies (9-14). EHV-1-specific CD8 ϩ class I major histocompatibility complex (MHC)-restricted cytotoxic T lymphocytes (CTL) were identified in the peripheral blood mononuclear cells of infected horses, reaching maximal levels 2 to 3 weeks postinfection (15,16). Intranasal inoculation of mice with an attenuated EHV-1 strain, KyA, resulted in the production of a primary virus-specific CTL response in the draining mediastinal lymph nodes (17). In this model, EHV-1-specific CTL activity was mediated by class I MHC-restricted CD8 ϩ T cells (17). The nonpathogenic EHV-1 strain Kentucky A (KyA) is attenuated in mice and horses, whereas the wild-type pathogenic strain RacL11 induces severe inflammatory cell infiltration in the lung, such that infected mice succumb at 4 to 6 days postinfection (dpi) (Fig. 1) (18-23). RacL11 was isolated from an aborted fetus (24). KyA has a long history of passage outside the natural host and

show abstract

The murine homolog of the HIN 200 family, Ifi 204, is constitutively expressed in myeloid cells and selectively induced in the monocyte/macrophage lineage

Cited by 38 publications

References 32 publications

Transcription and Growth Regulatory Functions of the HIN-200 Family of Proteins

Transcription and Growth Regulatory Functions of the HIN-200 Family of Proteins

Immunohistochemical Expression Analysis of the Human Interferon-Inducible Gene IFI16, a Member of the HIN200 Family, Not Restricted to Hematopoietic Cells

Immunization with Attenuated Equine Herpesvirus 1 Strain KyA Induces Innate Immune Responses That Protect Mice from Lethal Challenge

Contact Info

Product

Resources

About