The Multixenobiotic resistance system as a possible protective response triggered by microplastic ingestion in Mediterranean mussels (Mytilus galloprovincialis): Larvae and adult stages

Franzellitti, Silvia; Capolupo, Marco; Wathsala, Rajapaksha Haddokara Gedara Rasika; Valbonesi, Paola; Fabbri, Elena

doi:10.1016/j.cbpc.2019.02.005

Cited by 25 publications

(13 citation statements)

References 59 publications

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“…One of the most concerning risks of the MP and NP uptake is related to their potential to interfere with innate immune responses, altering the organisms’ defense mechanisms toward microbiological stressors [ 55 , 56 , 57 , 58 ]. In bivalves, the hemolymph is the major biological compartment involved in the immune response and recent evidence indicates clear concentration-dependent trends of migration and accumulation of PS particles in this tissue, even after short-term (4-day) exposures [ 17 ]. Within the hemolymph, the first line of defense toward host interactions is represented by the extracellular and intracellular degradation mechanisms governed by the hemocytes, including the lysozyme extracellular release and the phagocytosis of non-self material [ 59 , 60 ].…”

Section: Discussionmentioning

confidence: 99%

“…The main documented alterations include gut occlusion and reduced feeding stimulus (associated with reduced growth/fertility) in turtles and seabirds [ 9 , 10 ], metabolic and reproductive disorders in fish [ 11 , 12 ], and behavioral/nutritional alterations in invertebrates, like arthropods, annelids, and mollusks [ 13 , 14 , 15 ]. Besides, recent studies on bivalves report the alterations of cellular, biochemical and ontogenetic pathways, accenting the risks posed by MP on the processes governing the physiological adaptation to external stressors and population recruitment patterns [ 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

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A Comparative Assessment of the Chronic Effects of Micro- and Nano-Plastics on the Physiology of the Mediterranean Mussel Mytilus galloprovincialis

2021

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The ocean contamination caused by micro- and nano-sized plastics is a matter of increasing concern regarding their potential effects on marine organisms. This study compared the effects of a 21-day exposure to 1.5, 15, and 150 ng/L of polystyrene microplastics (PS-MP, 3-µm) and nanoplastics (PS-NP, 50-nm) on a suite of biomarkers measured in the Mediterranean mussel Mytilus galloprovincialis. Endpoints encompassed immunological/lysosomal responses, oxidative stress/detoxification parameters, and neurotoxicological markers. Compared to PS-MP, PS-NP induced higher effects on lysosomal parameters of general stress. Exposures to both particle sizes increased lipid peroxidation and catalase activity in gills; PS-NP elicited greater effects on the phase-II metabolism enzyme glutathione S-transferase and on lysozyme activity, while only PS-MP inhibited the hemocyte phagocytosis, suggesting a major role of PS particle size in modulating immunological/detoxification pathways. A decreased acetylcholinesterase activity was induced by PS-NP, indicating their potential to impair neurological functions in mussels. Biomarker data integration in the Mussel Expert System identified an overall greater health status alteration in mussels exposed to PS-NP compared to PS-MP. This study shows that increasing concentrations of nanoplastics may induce higher effects than microplastics on the mussel’s lysosomal, metabolic, and neurological functions, eventually resulting in a greater impact on their overall fitness.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Comparative Assessment of the Chronic Effects of Micro- and Nano-Plastics on the Physiology of the Mediterranean Mussel Mytilus galloprovincialis

2021

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“…According to Nobili et al the ABCC4 (MRP4) expression is highly associated with clinical sequels in patients with neuroblastoma [8]. Recently, studies have reported the upregulation of ABC transporters genes by pollutants, highlighting how important these proteins are in building resistance toward chemotherapeutics [33][34][35]. Thus, the current study suggests that BDE-209 could play an important role in cancer disease.…”

Section: Discussionmentioning

confidence: 56%

BDE-209 and TCDD Modulate the Expression and Activity of ATP-Binding Cassette (ABC) Transporters in Murine Melanoma Cells (B16-F1)

Marchi¹,

Moggio²,

Neto³

et al. 2021

IJCST

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Persistent organic pollutants (POPs) may alter tumor cells phenotype, possibly increasing malignancy, but there is a lack of studies investigating the mechanisms by which POPs may affect tumor cells. The ATP-Binding Cassette (ABC) transporter proteins are a widely studied component of drug resistance and tumor progression. We hypothesized that the levels of BDE-209 and TCDD detected in human serum can modulate the gene expression or activity of ATP-binding cassette (ABC) transporters in murine melanoma (B16-F1) cells. In this study, we observed an upregulation of the ABCB1 and ABCC4 (24 h) genes followed by an increased protein activity after BDE-209 15 day-exposure. We also observed that cells exposed to TCDD showed an upregulation of ABCB5, ABCC1 and ABCC4 genes (24 h) and change of protein activity after 15 days of exposure. These findings suggest that BDE-209 and TCDD can regulate the phenotype of B16-F1 cells by interfering with the expression and activity of ATP-binding cassette (ABC) transporters. This investigation revealed that environmental pollutants might intervene and modify cells’ resistance to chemotherapy and cancer prognosis.

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“…Endpoints related to oxidative stress were the second most common endpoint, with 14 studies, 8 of which showing impacts on lipid peroxidation, formation of reactive oxygen species and total oxyradical scavenging capacity (Avio et al 2015;Brandts et al 2018b;Gonçalves et al 2019;González-Fernández et al 2018;Guilhermino et al 2018;Magni et al 2018;Oliveira et al 2018;Paul-Pont et al 2016;Revel et al 2019;Ribeiro et al 2017;Santana et al 2018;Song et al 2019;Sussarellu et al 2016;von Moos et al 2012). In combination with oxidative stress, alteration in enzymatic activity was also one of the main endpoints determined in molluscs (reported in 12 studies), with 10 studies showing alterations to antioxidant enzymes (Avio et al 2015;Brandts et al 2018b;Franzellitti et al 2019;Gonçalves et al 2019;Guilhermino et al 2018;Magni et al 2018;Oliveira et al 2018;Paul-Pont et al 2016;Pittura et al 2018;Revel et al 2019;Ribeiro et al 2017;Song et al 2019). Alterations in gene expression were also a common endpoint in most of the reviewed studies (12 studies), with 10 reporting up-and downregulation of genes involved in different metabolic pathways as detoxification, immunity, apoptosis, energy reserves, etc.…”

Section: Molluscamentioning

confidence: 99%

“…Alterations in gene expression were also a common endpoint in most of the reviewed studies (12 studies), with 10 reporting up-and downregulation of genes involved in different metabolic pathways as detoxification, immunity, apoptosis, energy reserves, etc. (Avio et al 2015;Balbi et al 2017;Brandts et al 2018a;Capolupo et al 2018;Gallardo-Escárate 2017, 2018;Franzellitti et al 2019;Paul-Pont et al 2016;Pittura et al 2018;Revel et al 2019;Rochman et al 2017;Sussarellu et al 2016). Histopathological alterations were also included in some of these studies to understand the effects of particle ingestion in different organs (total nine studies), with five studies reporting alterations in the gills and digestive glands of exposed organisms (Bråte et al 2018;Gardon et al 2018;Gonçalves et al 2019;Guilhermino et al 2018;Paul-Pont et al 2016;Revel et al 2019;Rochman et al 2017;Song et al 2019;von Moos et al 2012).…”

Section: Molluscamentioning

confidence: 99%

Ecotoxicological Impacts of Micro- and Nanoplastics in Terrestrial and Aquatic Environments

Gomes

Bour

Coutris

et al. 2021

Microplastic in the Environment: Pattern and Process

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Plastic pollution is a widespread environmental problem that is currently one of the most discussed issues by scientists, policymakers and society at large. The potential ecotoxicological effects of plastic particles in a wide range of organisms have been investigated in a growing number of exposure studies over the past years. Nonetheless, many questions still remain regarding the overall effects of microplastics and nanoplastics on organisms from different ecosystem compartments, as well as the underlying mechanisms behind the observed toxicity. This chapter provides a comprehensive literature review on the ecotoxicological impacts of microplastics and nanoplastics in terrestrial and aquatic organisms in the context of particle characteristics, interactive toxicological effects, taxonomic gradients and with a focus on synergies with associated chemicals. Overall, a total of 220 references were reviewed for their fulfilment of specific quality criteria (e.g. experimental design, particle characteristics, ecotoxicological endpoints and findings), after which 175 were included in our assessment. The analysis of the reviewed studies revealed that organisms’ responses were overall influenced by the physicochemical heterogeneity of the plastic particles used, for which distinct differences were attributed to polymer type, size, morphology and surface alterations. On the other hand, little attention has been paid to the role of additive chemicals in the overall toxicity. There is still little consistency regarding the biological impacts posed by plastic particles, with observed ecotoxicological effects being highly dependent on the environmental compartment assessed and specific morphological, physiological and behavioural traits of the species used. Nonetheless, evidence exists of impacts across successive levels of biological organization, covering effects from the subcellular level up to the ecosystem level. This review presents the important research gaps concerning the ecotoxicological impacts of plastic particles in different taxonomical groups, as well as recommendations on future research priorities needed to better understand the ecological risks of plastic particles in terrestrial and aquatic environments.

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The Multixenobiotic resistance system as a possible protective response triggered by microplastic ingestion in Mediterranean mussels (Mytilus galloprovincialis): Larvae and adult stages

Cited by 25 publications

References 59 publications

A Comparative Assessment of the Chronic Effects of Micro- and Nano-Plastics on the Physiology of the Mediterranean Mussel Mytilus galloprovincialis

A Comparative Assessment of the Chronic Effects of Micro- and Nano-Plastics on the Physiology of the Mediterranean Mussel Mytilus galloprovincialis

BDE-209 and TCDD Modulate the Expression and Activity of ATP-Binding Cassette (ABC) Transporters in Murine Melanoma Cells (B16-F1)

Ecotoxicological Impacts of Micro- and Nanoplastics in Terrestrial and Aquatic Environments

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