The multistructural forms of box C/D ribonucleoprotein particles

Yu, Ge; Zhao, Yu

doi:10.1261/rna.068312.118

Cited by 35 publications

(33 citation statements)

References 73 publications

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“…Biophysical studies showed that SNORD-RNPs exhibit a large structural diversity generated by forming dimers of a monomer SNORD-RNP (Fig. 1), which also suggests diverse functions [42].…”

Section: Transcription Biogenesis and Post-transcriptional Modificatmentioning

confidence: 99%

C/D box snoRNAs in viral infections: RNA viruses use old dogs for new tricks

Stamm

Lodmell

2019

Non-coding RNA Research

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C/D box snoRNAs (SNORDs) are a highly expressed class of non-coding RNAs. Besides their well-established role in rRNA modification, C/D box snoRNAs form protein complexes devoid of fibrillarin and regulate pre-mRNA splicing and polyadenylation of numerous genes. There is an emerging body of evidence for functional interactions between RNA viruses and C/D box snoRNAs. The infectivity of some RNA viruses depends on enzymatically active fibrillarin, and many RNA viral proteins associate with nucleolin or nucleophosmin, suggesting that viruses benefit from their cytosolic accumulation. These interactions are likely reflected by morphological changes in the nucleolus, often leading to relocalization of nucleolar proteins and ncRNAs to the cytosol that are a characteristic feature of viral infections. Knock-down studies have also shown that RNA viruses need specific C/D box snoRNAs for optimal replication, suggesting that RNA viruses benefit from gene expression programs regulated by SNORDs, or that viruses have evolved “new” uses for these humble ncRNAs to advance their prospects during infection.

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“…Biophysical studies showed that SNORD-RNPs exhibit a large structural diversity generated by forming dimers of a monomer SNORD-RNP (Fig. 1), which also suggests diverse functions [42].…”

Section: Transcription Biogenesis and Post-transcriptional Modificatmentioning

confidence: 99%

C/D box snoRNAs in viral infections: RNA viruses use old dogs for new tricks

Stamm

Lodmell

2019

Non-coding RNA Research

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“…snoRNAs are essential small RNA molecules that have been identified to regulate and guide the posttranscriptional chemical modifications of rRNA, namely 2′O-methylation and pseudouridylation [ 20 , 21 , 22 ]. They are found in many organisms including archaea (despite the absence of a nucleolus) and eukaryotes but are notably absent in bacteria.…”

Section: Introductionmentioning

confidence: 99%

The Existence and Localization of Nuclear snoRNAs in Arabidopsis thaliana Revisited

Streit

Shanmugam

Garbelyanski

et al. 2020

Plants

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Ribosome biogenesis is one cell function-defining process. It depends on efficient transcription of rDNAs in the nucleolus as well as on the cytosolic synthesis of ribosomal proteins. For newly transcribed rRNA modification and ribosomal protein assembly, so-called small nucleolar RNAs (snoRNAs) and ribosome biogenesis factors (RBFs) are required. For both, an inventory was established for model systems like yeast and humans. For plants, many assignments are based on predictions. Here, RNA deep sequencing after nuclei enrichment was combined with single molecule species detection by northern blot and in vivo fluorescence in situ hybridization (FISH)-based localization studies. In addition, the occurrence and abundance of selected snoRNAs in different tissues were determined. These approaches confirm the presence of most of the database-deposited snoRNAs in cell cultures, but some of them are localized in the cytosol rather than in the nucleus. Further, for the explored snoRNA examples, differences in their abundance in different tissues were observed, suggesting a tissue-specific function of some snoRNAs. Thus, based on prediction and experimental confirmation, many plant snoRNAs can be proposed, while it cannot be excluded that some of the proposed snoRNAs perform alternative functions than are involved in rRNA modification.

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“…6A). Among the proteins encoded by these genes, NOL-56 is an ortholog of human NOP56, which binds snoRNAs and facilitates box C/D ribonucleoprotein-guided methyltransferase activity [27]. FIB-1 encodes the C. elegans ortholog of human fibrillarin and Saccharomyces cerevisiae Nop1p.…”

Section: Resultsmentioning

confidence: 99%

Co-surveillance of ribosomal RNA by the exosome complex and nucleolar RNAi inC. elegans

Liao

Chen

et al. 2020

Preprint

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Eukaryotic cells express a wide variety of endogenous small regulatory RNAs that function in the nucleus. We previously found that erroneous rRNAs induce the generation of antisense ribosomal siRNAs (risiRNAs) which silence the expression of rRNAs via the nuclear RNAi defective (Nrde) pathway. To further understand the biological roles and mechanisms of this class of small regulatory RNAs, we conducted forward genetic screening to identify factors involved in risiRNA generation in Caenorhabditis elegans. We found that risiRNAs accumulated in the RNA exosome mutants. risiRNAs directed a NRDE-dependent silencing of pre-rRNAs in the nucleolus. In the presence of risiRNA, NRDE-2 accumulated in the nucleolus and colocalized with RNA polymerase I. risiRNA inhibited the transcription elongation of RNA polymerase I by decreasing RNAP I occupancy downstream of the site of RNAi. Meanwhile, exosome mislocalized from the nucleolus to nucleoplasm in suppressor of siRNA (susi) mutants, in which erroneous rRNAs accumulated. These results establish a novel model of rRNA surveillance by combining ribonuclease-mediated RNA degradation with small RNA-directed nucleolar RNAi system.

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The multistructural forms of box C/D ribonucleoprotein particles

Cited by 35 publications

References 73 publications

C/D box snoRNAs in viral infections: RNA viruses use old dogs for new tricks

C/D box snoRNAs in viral infections: RNA viruses use old dogs for new tricks

The Existence and Localization of Nuclear snoRNAs in Arabidopsis thaliana Revisited

Co-surveillance of ribosomal RNA by the exosome complex and nucleolar RNAi inC. elegans

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