The Multiple Roles of Hypothetical Gene BPSS1356 in Burkholderia pseudomallei

Yam, Hokchai; Rahim, Ainihayati Abdul; Mohamad, Suriani; Mahadi, Nor Muhammad; Manaf, Uyub Abdul; Shu‐Chien, Alexander Chong; Najimudin, Nazalan

doi:10.1371/journal.pone.0099218

Cited by 3 publications

(1 citation statement)

References 71 publications

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“…Members of the σ 70 family are responsible for the expression of all essential genes, while members of the σ 54 family are mostly involved in nitrogen metabolism-associated genes. B. pseudomallei contains several σ factors, including RpoC ( σ C) [ 57 ], RpoN ( σ N) [ 58 ], RpoE ( σ E) [ 30 ], and RpoS ( σ S) [ 34 ]. RpoE and RpoS are members of the σ 70 family which play an important role in response to extracellular stress [ 55 ].…”

Section: Molecular Mechanisms For B Pseudomallei mentioning

confidence: 99%

Burkholderia pseudomalleiAdaptation for Survival in Stressful Conditions

Duangurai

Indrawattana

Pumirat

2018

BioMed Research International

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Burkholderia pseudomallei is a Gram-negative bacterium that causes melioidosis, which can be fatal in humans. Melioidosis is prevalent in the tropical regions of Southeast Asia and Northern Australia. Ecological data have shown that this bacterium can survive as a free-living organism in environmental niches, such as soil and water, as well as a parasite living in host organisms, such as ameba, plants, fungi, and animals. This review provides an overview of the survival and adaptation of B. pseudomallei to stressful conditions induced by hostile environmental factors, such as salinity, oxidation, and iron levels. The adaptation of B. pseudomallei in host cells is also reviewed. The adaptive survival mechanisms of this pathogen mainly involve modulation of gene and protein expression, which could cause alterations in the bacteria's cell membrane, metabolism, and virulence. Understanding the adaptations of this organism to environmental factors provides important insights into the survival and pathogenesis of B. pseudomallei, which may lead to the development of novel strategies for the control, prevention, and treatment of melioidosis in the future.

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Section: Molecular Mechanisms For B Pseudomallei mentioning

confidence: 99%

Burkholderia pseudomalleiAdaptation for Survival in Stressful Conditions

Duangurai

Indrawattana

Pumirat

2018

BioMed Research International

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Disruption of the Burkholderia pseudomallei two-component signal transduction system BbeR-BbeS leads to increased extracellular DNA secretion and altered biofilm formation

Alwis

Treerat

Gong

et al. 2020

Veterinary Microbiology

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Genome-scale analysis of the genes that contribute to Burkholderia pseudomallei biofilm formation identifies a crucial exopolysaccharide biosynthesis gene cluster

et al. 2017

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Burkholderia pseudomallei, the causative agent of melioidosis, is an important public health threat due to limited therapeutic options for treatment. Efforts to improve therapeutics for B. pseudomallei infections are dependent on the need to understand the role of B. pseudomallei biofilm formation and its contribution to antibiotic tolerance and persistence as these are bacterial traits that prevent effective therapy. In order to reveal the genes that regulate and/or contribute to B. pseudomallei 1026b biofilm formation, we screened a sequence defined two-allele transposon library and identified 118 transposon insertion mutants that were deficient in biofilm formation. These mutants include transposon insertions in genes predicted to encode flagella, fimbriae, transcriptional regulators, polysaccharides, and hypothetical proteins. Polysaccharides are key constituents of biofilms and B. pseudomallei has the capacity to produce a diversity of polysaccharides, thus there is a critical need to link these biosynthetic genes with the polysaccharides they produce to better understand their biological role during infection. An allelic exchange deletion mutant of the entire B. pseudomallei biofilm-associated exopolysaccharide biosynthetic cluster was decreased in biofilm formation and produced a smooth colony morphology suggestive of the loss of exopolysaccharide production. Conversely, deletion of the previously defined capsule I polysaccharide biosynthesis gene cluster increased biofilm formation. Bioinformatics analyses combined with immunoblot analysis and glycosyl composition studies of the partially purified exopolysaccharide indicate that the biofilm-associated exopolysaccharide is neither cepacian nor the previously described acidic exopolysaccharide. The biofilm-associated exopolysaccharide described here is also specific to the B. pseudomallei complex of bacteria. Since this novel exopolysaccharide biosynthesis cluster is retained in B. mallei, it is predicted to have a role in colonization and infection of the host. These findings will facilitate further advances in understanding the pathogenesis of B. pseudomallei and improve diagnostics and therapeutic treatment strategies.

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The Multiple Roles of Hypothetical Gene BPSS1356 in Burkholderia pseudomallei

Cited by 3 publications

References 71 publications

Burkholderia pseudomalleiAdaptation for Survival in Stressful Conditions

Burkholderia pseudomalleiAdaptation for Survival in Stressful Conditions

Disruption of the Burkholderia pseudomallei two-component signal transduction system BbeR-BbeS leads to increased extracellular DNA secretion and altered biofilm formation

Genome-scale analysis of the genes that contribute to Burkholderia pseudomallei biofilm formation identifies a crucial exopolysaccharide biosynthesis gene cluster

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