Disruption of the Burkholderia pseudomallei two-component signal transduction system BbeR-BbeS leads to increased extracellular DNA secretion and altered biofilm formation

Alwis, Priyangi A; Treerat, Puthayalai; Gong, Lan; Lucas, Deanna Deveson; Allwood, Elizabeth M.; Prescott, Mark; Devenish, Rodney J.; Adler, Ben; Boyce, John D.

doi:10.1016/j.vetmic.2020.108603

Cited by 4 publications

(8 citation statements)

References 33 publications

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“…Much like Salmonella [68], Burkholderia is capable of producing either a surface adherent biofilm or a pellicle (Fig 1a-c). This phenomenon was also demonstrated in a previous Burkholderiastudy which showed both pellicle and adherent biofilm [23]. The conditions and reasons for the change from surface adherent to pellicle biofilm remains unknown in Burkholderia .…”

Section: Discussionsupporting

confidence: 67%

“…Biofilm is involved in the antibiotic resistance of bacterial disease, relapse, and persistent cells [65,66]. eDNA exportation has been observed in both Grampositive and Gram-negative bacteria and has now been associated with biofilm facilitation and composition [23,67].…”

Section: Discussionmentioning

confidence: 99%

“…In Salmonella , curli is responsible for the switch [68] whereas, in C. crescentus, biofilm is initially formed as a monolayer of cells that subsequently develop into a three-dimensional structure and as the biofilm matures, it becomes more cohesive and less adherent [69]. Previous studies have confirmed that some pellicles of some organisms are composed of eDNA with close cell contact [23], sucrose, and proteins [70].…”

Section: Discussionmentioning

confidence: 99%

“…To survive under stressed environments like the human host, B. pseudomallei modifies the expression of virulence genes like the Bsa type III secretion system [19], metabolic and motility genes [20], and biofilm genes [21]. A variety of genes have been implicated in B. pseudomallei biofilm processes like adhesion [22] and eDNA secretion which facilitate biofilm formation [23,24]. However, the complete pathways for genes involved in the formation of biofilms and/or the secretion of molecules are not clearly understood.…”

Section: Introductionmentioning

confidence: 99%

“…Numerous studies have shown that eDNA is released from viable cells and required for biofilm formation and structure inBurkholderia . [23,25,26]. Burkholderia species also produce and export numerous outer membrane polysaccharides and proteins [27][28][29].…”

Section: Introductionmentioning

confidence: 99%

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Production and molecular composition of Burkholderia pseudomallei and Burkholderia thailandensis biofilms

Okaro

Mou

DeShazer

2021

Preprint

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Biofilm communities cause many infectious diseases. Biofilms are diverse microbial species found either attached to a surface or aggregated into an extracellular matrix. Bacteria form biofilms as a default mode of growth or as a response mechanism to environmental conditions like stress. As such, biofilm strains are increasingly virulent causing a wide variety of chronic persistent diseases, are typically antibiotic-resistant and known to improve host mortality rate. Most biofilms contain polysaccharides, proteins, extracellular DNA (eDNA), RNA, and water. Determining and quantifying the major components of a biofilm may indicate an appropriate treatment for biofilm eradication. Burkholderia pseudomallei is a Gram-negative, motile bacillus typically found in surface water and/or soil in endemic regions. It is the etiologic agent of melioidosis and is capable of forming both surface adherent and air-liquid interface biofilms (pellicle) in broth cultures. This study evaluates the components of established biofilms using B. pseudomallei and Burkholderia thailandensis, a closely related nonpathogenic species. Using assays, fluorescent dyes and microscopy, we quantified the major components of biofilms produced by five genetically related B. pseudomallei strains and compared them to B. thailandensis E264. Our data show that biofilm produced by the B. pseudomallei 1026b derivatives and B. thailandensis E264 significantly differ. The molecular composition of the surface adherent biofilm is similar to the molecular composition of the air-liquid pellicle. Finally, the eDNA quantity biofilm produced by JW270 which bears a CPS I deletion, is significantly increased in comparison to 1026 and Bp82 biofilm.

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Section: Discussionsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Production and molecular composition of Burkholderia pseudomallei and Burkholderia thailandensis biofilms

Okaro

Mou

DeShazer

2021

Preprint

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Enhancement of Livestock Wastewater Treatment by a Novel Wooden-modified Biocarrier

Li,

Feng,

et al. 2024

Environmental Pollution

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Analysis of the role of the QseBC two-component sensory system in epinephrine-induced motility and intracellular replication of Burkholderia pseudomallei

et al. 2023

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Burkholderia pseudomallei is a facultative intracellular bacterial pathogen that causes melioidosis, a severe invasive disease of humans. We previously reported that the stress-related catecholamine hormone epinephrine enhances motility of B. pseudomallei, transcription of flagellar genes and the production of flagellin. It has been reported that the QseBC two-component sensory system regulates motility and virulence-associated genes in other Gram-negative bacteria in response to stress-related catecholamines, albeit disparities between studies exist. We constructed and whole-genome sequenced a mutant of B. pseudomallei with a deletion spanning the predicted qseBC homologues (bpsl0806 and bpsl0807). The ΔqseBC mutant exhibited significantly reduced swimming and swarming motility and reduced transcription of fliC. It also exhibited a defect in biofilm formation and net intracellular survival in J774A.1 murine macrophage-like cells. While epinephrine enhanced bacterial motility and fliC transcription, no further reduction in these phenotypes was observed with the ΔqseBC mutant in the presence of epinephrine. Plasmid-mediated expression of qseBC suppressed bacterial growth, complicating attempts to trans-complement mutant phenotypes. Our data support a role for QseBC in motility, biofilm formation and net intracellular survival of B. pseudomallei, but indicate that it is not essential for epinephrine-induced motility per se.

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Disruption of the Burkholderia pseudomallei two-component signal transduction system BbeR-BbeS leads to increased extracellular DNA secretion and altered biofilm formation

Cited by 4 publications

References 33 publications

Production and molecular composition of Burkholderia pseudomallei and Burkholderia thailandensis biofilms

Production and molecular composition of Burkholderia pseudomallei and Burkholderia thailandensis biofilms

Enhancement of Livestock Wastewater Treatment by a Novel Wooden-modified Biocarrier

Analysis of the role of the QseBC two-component sensory system in epinephrine-induced motility and intracellular replication of Burkholderia pseudomallei

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