The multigene family of fungal laccases and their expression in the white rot basidiomycete Flammulina velutipes

Wang, Wei; Liu, Fang; Jiang, Yuji; Wu, Guangmei; Guo, Lixian; Chen, Renliang; Chen, Bingzhi; Lu, Yuanping; Dai, Yu-Cheng; Xie, Baogui

doi:10.1016/j.gene.2015.03.020

Cited by 59 publications

(67 citation statements)

References 53 publications

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“…New HD and PR loci of F. velutipes were identified using the draft genomes of two mating-compatible strains; F. velutipes strain L11 and F. velutipes strain W23 (Liu et al 2015; Wang et al 2015b; Zeng et al 2015). The draft genome of strain L11 consisted of 1858 scaffolds covering 34.75 Mb and 11,526 predicted genes.…”

Section: Resultsmentioning

confidence: 99%

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Advances in Understanding Mating Type Gene Organization in the Mushroom-Forming FungusFlammulina velutipes

Wang

Lian

et al. 2016

G3 Genes|Genomes|Genetics

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The initiation of sexual development in the important edible and medicinal mushroom Flammulina velutipes is controlled by special genes at two different, independent, mating type (MAT) loci: HD and PR. We expanded our understanding of the F. velutipes mating type system by analyzing the MAT loci from a series of strains. The HD locus of F. velutipes houses homeodomain genes (Hd genes) on two separated locations: sublocus HD-a and HD-b. The HD-b subloci contained strain-specific Hd1/Hd2 gene pairs, and crosses between strains with different HD-b subloci indicated a role in mating. The function of the HD-a sublocus remained undecided. Many, but not all strains contained the same conserved Hd2 gene at the HD-a sublocus. The HD locus usually segregated as a whole, though we did detect one new HD locus with a HD-a sublocus from one parental strain, and a HD-b sublocus from the other. The PR locus of F. velutipes contained pheromone receptor (STE3) and pheromone precursor (Pp) genes at two locations, sublocus PR-a and PR-b. PR-a and PR-b both contained sets of strain-specific STE3 and Pp genes, indicating a role in mating. PR-a and PR-b cosegregated in our experiments. However, the identification of additional strains with identical PR-a, yet different PR-b subloci, demonstrated that PR subloci can recombine within the PR locus. In conclusion, at least three of the four MAT subloci seem to participate in mating, and new HD and PR loci can be generated through intralocus recombination in F. velutipes.

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Section: Resultsmentioning

confidence: 99%

“…Kong of the Rural Development Administration (RDA) in Korea. Genome sequences of F. velutipes strain L11 and strain W23 were obtained from our lab (Zeng et al 2015; Liu et al 2015; Wang et al 2015b). Genomic DNA was extracted from mycelium using a CTAB method (Stajich et al 2010).…”

Section: Methodsmentioning

confidence: 99%

Advances in Understanding Mating Type Gene Organization in the Mushroom-Forming FungusFlammulina velutipes

Wang

Lian

et al. 2016

G3 Genes|Genomes|Genetics

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“…APHZ00000000; BioProject: 191864). For analysis of gene structure, all of the transcriptome reads at each developmental stage [39,41] were mapped to the full-length Fv-JRL1 gene, as well as 2 kbp each of upstream and downstream sequences, using Zoom lite with a maximum of 40 mismatched base pairs [39,42]. In addition, comparison of the sequences of Fv-JRL1 in W23 and L11 (GenBank Accession No.…”

Section: Methodsmentioning

confidence: 99%

“…F. velutipes strain H1123 was cultured on composted substrate prepared as described by Wang et al [41]. Collection of mycelia and samples at different developmental stages was performed according to the method of Wang et al [41], after which all samples were immediately frozen in liquid nitrogen.…”

Section: Methodsmentioning

confidence: 99%

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A Jacalin-Related Lectin Regulated the Formation of Aerial Mycelium and Fruiting Body in Flammulina velutipes

Chen

Long

et al. 2016

IJMS

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Flammulina velutipes, one of the most popular mushroom species in the world, has been recognized as a useful model system to study the biochemical and physiological aspects of the formation and elongation of fruit body. However, few reports have been published on the regulation of fruiting body formation in F. velutipes at the molecular level. In this study, a jacalin-related lectin gene from F. velutipes was characterized. The phylogenetic tree revealed that Fv-JRL1 clustered with other basidiomycete jacalin-like lectins. Moreover, the transcriptional pattern of the Fv-JRL1 gene in different developmental stages of F. velutipes implied that Fv-JRL1 could be important for formation of fruit body. Additionally, RNA interference (RNAi) and overexpression analyses provided powerful evidence that the lectin gene Fv-JRL1 from F. velutipes plays important roles in fruiting body formation.

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Screening candidate genes related to volatile synthesis in shiitake mushrooms and construction of regulatory networks to effectively improve mushroom aroma

Chen

Wang

et al. 2021

J Sci Food Agric

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BACKGROUND: Metabolite formation is a physiological stress response during the growth and development of shiitake mushrooms (Lentinula edodes). The characteristic flavor metabolites are important quality components in shiitake mushrooms. To investigate the formation mechanisms of characteristic flavor metabolites, transcriptome analyses were performed on shiitake mushrooms harvested at different growth stages. RESULTS: In total, 30 genes related to the synthesis of characteristic volatiles of mushrooms were identified via screening. Through KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis of the selected genes and correlation analyses of gene expressions, the main volatile synthesis pathways were determined as histidine metabolism, glutathione metabolism and biosynthesis of unsaturated fatty acids. Gene cluster and correlation analyses were performed to clarify the combined effects of different genes in the enzymatic reactions. Further, a correlation network of candidate genes was built based on the gene expression levels. CONCLUSION:The activities of flavor synthases and the content of characteristic flavor metabolites were analyzed; the enzyme activity changes and metabolic product distribution sites were clarified. A synthesis and regulation network was constructed for the candidate genes and characteristic volatiles, and information was obtained for 16 hub genes. Moreover, it was essential to identify and characterize the key genes and synthases involved in the synthesis of the characteristic volatiles of mushrooms. This information provides us with a better understanding of the biosynthesis and regulation of the volatiles, which will lay the foundation for improving the quality of shiitake mushrooms.

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The multigene family of fungal laccases and their expression in the white rot basidiomycete Flammulina velutipes

Cited by 59 publications

References 53 publications

Advances in Understanding Mating Type Gene Organization in the Mushroom-Forming FungusFlammulina velutipes

Advances in Understanding Mating Type Gene Organization in the Mushroom-Forming FungusFlammulina velutipes

A Jacalin-Related Lectin Regulated the Formation of Aerial Mycelium and Fruiting Body in Flammulina velutipes

Screening candidate genes related to volatile synthesis in shiitake mushrooms and construction of regulatory networks to effectively improve mushroom aroma

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