A Jacalin-Related Lectin Regulated the Formation of Aerial Mycelium and Fruiting Body in Flammulina velutipes

Abstract

Flammulina velutipes, one of the most popular mushroom species in the world, has been recognized as a useful model system to study the biochemical and physiological aspects of the formation and elongation of fruit body. However, few reports have been published on the regulation of fruiting body formation in F. velutipes at the molecular level. In this study, a jacalin-related lectin gene from F. velutipes was characterized. The phylogenetic tree revealed that Fv-JRL1 clustered with other basidiomycete jacalin-like lectins. Moreover, the transcriptional pattern of the Fv-JRL1 gene in different developmental stages of F. velutipes implied that Fv-JRL1 could be important for formation of fruit body. Additionally, RNA interference (RNAi) and overexpression analyses provided powerful evidence that the lectin gene Fv-JRL1 from F. velutipes plays important roles in fruiting body formation.

A WD40 Protein Encoding Gene Fvcpc2 Positively Regulates Mushroom Development and Yield in Flammulina velutipes

“…The significant levels of Fvcpc2 RNAi#43 compared to wild type were calculated by t-test and marked as ** (0.001 < p < 0.01) or *** (p < 0.001). p v−JRL1 = 0.0025, n = 3; p gene9094 = 5.1650E-05, n = 3; p gene10415 = 7.2365E-05, n = 3; p gene10856 = 5.4826E-08, n = 3; p Hyd−1 = 0.0003, n = 3; p Hyd−3 = 0.0001, n = 3; p Hyd−4 = 1.7168E-05, n = 3; p Hyd−6 = 0.0002, n = 3; p FVFD30 = 1.1865E-05, n = 3; p FVFD16 = 0.0009, n = 3; p gene543 = 3.8621E-06, n = 3. basidioma development in F. velutipes (Lu et al, 2016). Our analysis showed that 4 of 9 lectin genes had difference in transcriptional levels between wild type and Fvcpc2 RNAi#43 .…”

“…In F. velutipes, the lectin encoding gene Fv-JRL1 and its three homolog encoding genes were dramatically upregulated at the primordium formation stage (Liu et al, 2015). F. velutipes Fv-JRL1 positively regulates hyphal growth and fruiting body development (Lu et al, 2016). Thus, enhanced lectin production might be required for normal mushroom formation.…”

“…Due to the available genetic techniques (Kim et al, 2010;Okamoto et al, 2010;Hatoh et al, 2013;Shi et al, 2016), the world-widely cultivated Winter Mushroom (F. velutipes, which was recently renamed as Flammulina filiformis; Wang et al, 2018) has been used as a model to study mushroom development (Park et al, 2014;Lu et al, 2016). In this study, we intended to test whether CPC-2 orthologs are functionally conserved for fruiting body development in the two phyla Ascomycota and Basidiomycota.…”

A Single Transcription Factor (PDD1) Determines Development and Yield of Winter Mushroom ( Flammulina velutipes )

“…The level of expression of jacalin-related lectin-encoding gene Fv-JRL1 was reduced in response to the pdd1 knockdown. According to the results of a previous study (39), Fv-JRL1 positively regulates basidioma development; its transcriptional level was dramatically increased in the primordial stage relative to its expression in the vegetative mycelia, and Fv-JRL1 knockdown severely impaired basidioma development. We found that two genes, gene 10415 and gene 10856, encoding Fv-JRL1 homologs, were also downregulated in the pdd1 knockdown (Data Set S1).…”

“…In F. velutipes, jacalin-related lectin-encoding gene Fv-JRL1 and the two corresponding homolog-encoding genes were upregulated dramatically at the primordium formation stage. Fv-JRL1 promotes hyphal growth and basidioma development (39). Lectin-encoding genes have also been previously shown to be associated with basidioma development in several other fungi (55).…”