A WD40 Protein Encoding Gene Fvcpc2 Positively Regulates Mushroom Development and Yield in Flammulina velutipes

Wu, Theodore Y.; Zhang, Zhenying; Hu, Chengcheng; Zhang, Long; Wei, Shenglong; Li, Shaojie

doi:10.3389/fmicb.2020.00498

Cited by 15 publications

(6 citation statements)

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“…Genetic manipulation of several cAMP and connected pathway components in S. commune resulted in altered fruiting characteristics, either repression or complete lack of fruiting body formation ( Yamagishi et al 2002 , 2004 , Knabe et al 2013 , Pelkmans 2016 ). The cAMP pathway has also been linked to morphogenesis in F. velutipes: the deletion of a Gβ-like protein CPC-2 completely impaired fruiting body formation, but the addition of cAMP or its analog 8-Bromo-cAMP (also a PKA-activator) into the medium restored fruiting in Fv.cpc2 knockdown mutants ( Wu et al 2020b ). Ras and G-protein - subunits and their regulators ( e.g.…”

Section: Results: Developmentally Expressed Gene Classes In the ...mentioning

confidence: 99%

Lessons on fruiting body morphogenesis from genomes and transcriptomes of Agaricomycetes

Nagy

Vonk

Künzler

et al. 2023

Studies in Mycology

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Fruiting bodies (sporocarps, sporophores or basidiomata) of mushroom-forming fungi (Agaricomycetes) are among the most complex structures produced by fungi. Unlike vegetative hyphae, fruiting bodies grow determinately and follow a genetically encoded developmental program that orchestrates their growth, tissue differentiation and sexual sporulation. In spite of more than a century of research, our understanding of the molecular details of fruiting body morphogenesis is still limited and a general synthesis on the genetics of this complex process is lacking. In this paper, we aim at a comprehensive identification of conserved genes related to fruiting body morphogenesis and distil novel functional hypotheses for functionally poorly characterised ones. As a result of this analysis, we report 921 conserved developmentally expressed gene families, only a few dozens of which have previously been reported to be involved in fruiting body development. Based on literature data, conserved expression patterns and functional annotations, we provide hypotheses on the potential role of these gene families in fruiting body development, yielding the most complete description of molecular processes in fruiting body morphogenesis to date. We discuss genes related to the initiation of fruiting, differentiation, growth, cell surface and cell wall, defence, transcriptional regulation as well as signal transduction. Based on these data we derive a general model of fruiting body development, which includes an early, proliferative phase that is mostly concerned with laying out the mushroom body plan (via cell division and differentiation), and a second phase of growth via cell expansion as well as meiotic events and sporulation. Altogether, our discussions cover 1 480 genes of Coprinopsis cinerea, and their orthologs in Agaricus bisporus, Cyclocybe aegerita, Armillaria ostoyae, Auriculariopsis ampla, Laccaria bicolor, Lentinula edodes, Lentinus tigrinus, Mycena kentingensis, Phanerochaete chrysosporium, Pleurotus ostreatus, and Schizophyllum commune, providing functional hypotheses for ~10 % of genes in the genomes of these species. Although experimental evidence for the role of these genes will need to be established in the future, our data provide a roadmap for guiding functional analyses of fruiting related genes in the Agaricomycetes. We anticipate that the gene compendium presented here, combined with developments in functional genomics approaches will contribute to uncovering the genetic bases of one of the most spectacular multicellular developmental processes in fungi.

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Section: Results: Developmentally Expressed Gene Classes In the ...mentioning

confidence: 99%

Lessons on fruiting body morphogenesis from genomes and transcriptomes of Agaricomycetes

Nagy

Vonk

Künzler

et al. 2023

Studies in Mycology

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“…The mutation elevated intracellular cAMP level in S. commune (227), which might mechanistically explain the block in fruiting. An interesting G-protein b-subunit-like protein with seven WD40 domains, cpc2 was also reported to regulate intracellular cAMP level in F. velutipes (228). The knockdown of Fv.cpc2 completely impaired fruiting body formation in F. velutipes.…”

Section: Genetic Bases Of Fruiting Body Developmentmentioning

confidence: 99%

“…The knockdown of Fv.cpc2 completely impaired fruiting body formation in F. velutipes. CPC2 is a widely conserved protein among fungi, and Fv.cpc2 was able to complement the sexual developmental defects caused by cpc2 deletion in N. crassa (228). Its C. neoformans ortholog, Gib2, was reported to function as an alternative G-protein b-subunit (229).…”

Section: Genetic Bases Of Fruiting Body Developmentmentioning

confidence: 99%

Evolutionary Morphogenesis of Sexual Fruiting Bodies in Basidiomycota: Toward a New Evo-Devo Synthesis

Virágh

Merényi

Csernetics

et al. 2022

Microbiol Mol Biol Rev

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“…Genetic manipulation of several cAMP and connected pathway components in S. commune resulted in altered fruiting characteristics, either repression or complete lack of fruiting body formation (Yamagishi et al 2002(Yamagishi et al , 2004Knabe et al 2013;Pelkmans 2016). The cAMP pathway has also been linked to morphogenesis in F. velutipes: the deletion of a Gβ-like protein CPC-2 completely impaired fruiting body formation, but the addition of cAMP or it analog 8-Bromo-cAMP (also a PKA-activator) into the medium restored fruiting in Fv.cpc2 knockdown mutants (Wu et al 2020b). Ras and G-protein subunits and their regulators (e.g.…”

Section: Mapk Tor and Camp Pathwaysmentioning

confidence: 99%

Lessons on fruiting body morphogenesis from genomes and transcriptomes of Agaricomycetes

Nagy

Vonk

Künzler

et al. 2021

Preprint

View full text Add to dashboard Cite

Fruiting bodies of mushroom-forming fungi (Agaricomycetes) are among the most complex structures produced by fungi. Unlike vegetative hyphae, fruiting bodies grow determinately and follow a genetically encoded developmental program that orchestrates tissue differentiation, growth and sexual sporulation. In spite of more than a century of research, our understanding of the molecular details of fruiting body morphogenesis is limited and a general synthesis on the genetics of this complex process is lacking. In this paper, we aim to comprehensively identify conserved genes related to fruiting body morphogenesis and distill novel functional hypotheses for functionally poorly characterized genes. As a result of this analysis, we report 921 conserved developmentally expressed gene families, only a few dozens of which have previously been reported in fruiting body development. Based on literature data, conserved expression patterns and functional annotations, we provide informed hypotheses on the potential role of these gene families in fruiting body development, yielding the most complete description of molecular processes in fruiting body morphogenesis to date. We discuss genes related to the initiation of fruiting, differentiation, growth, cell surface and cell wall, defense, transcriptional regulation as well as signal transduction. Based on these data we derive a general model of fruiting body development, which includes an early, proliferative phase that is mostly concerned with laying out the mushroom body plan (via cell division and differentiation), and a second phase of growth via cell expansion as well as meiotic events and sporulation. Altogether, our discussions cover 1480 genes of Coprinopsis cinerea, and their orthologs in Agaricus bisporus, Cyclocybe aegerita, Armillaria ostoyae, Auriculariopsis ampla, Laccaria bicolor, Lentinula edodes, Lentinus tigrinus, Mycena kentingensis, Phanerochaete chrysosporium, Pleurotus ostreatus, and Schizophyllum commune, providing functional hypotheses for ~10% of genes in the genomes of these species. Although experimental evidence for the role of these genes will need to be established in the future, our data provide a roadmap for guiding functional analyses of fruiting related genes in the Agaricomycetes. We anticipate that the gene compendium presented here, combined with developments in functional genomics approaches will contribute to uncovering the genetic bases of one of the most spectacular multicellular developmental processes in fungi. Key words: functional annotation; comparative genomics; cell wall remodeling; development; fruiting body morphogenesis; mushroom; transcriptome

show abstract

A WD40 Protein Encoding Gene Fvcpc2 Positively Regulates Mushroom Development and Yield in Flammulina velutipes

Cited by 15 publications

References 91 publications

Lessons on fruiting body morphogenesis from genomes and transcriptomes of Agaricomycetes

Lessons on fruiting body morphogenesis from genomes and transcriptomes of Agaricomycetes

Evolutionary Morphogenesis of Sexual Fruiting Bodies in Basidiomycota: Toward a New Evo-Devo Synthesis

Lessons on fruiting body morphogenesis from genomes and transcriptomes of Agaricomycetes

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