The mRNA and miRNA profiles of goat bronchial epithelial cells stimulated by <i>Pasteurella multocida</i> strains of serotype A and D

An, Q.; Chen, Si; Zhang, Luyin; Zhang, Zhenxing; Cheng, Yiwen; Wu, Haotian; Liu, Ang; Chen, Zhe; Li, Bin; Chen, Jie; Zheng, Yiying; Man, Churiga; Chen, Qiaoling; Du, Lijun

doi:10.7717/peerj.13047

Cited by 5 publications

(7 citation statements)

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“…For example, mice challenged with P. multocida serotype D HN01 strain, of which mRNA expression profile showed that I l-10 ( p < 0.05) was significantly up-regulated in the challenge group’s mouse spleen, and results also revealed the high pertinence between I l-10 and the IL-1 family, suggesting the possibly antagonistic regulation of spleen inflammation might exist between them [ 35 ], and this finding was the same as the result of this research. Interestingly, compared with our result, a similar discovery was revealed in another study of goat bronchial epithelial cells stimulated by P. multocida serotype A and D [ 36 ], of which the result indicated that in the P. multocida strain serotype A-stimulated group, the significant KEGG pathways related to immunity were the GABAergic synapse and Toll-like receptor signaling pathway. While in the P. multocida strain serotype D-stimulated group, phagosome and B cell receptor signaling pathway were significantly enriched.…”

Section: Discussionsupporting

confidence: 91%

Up-Regulation of Interleukin-10 in Splenic Immune Response Induced by Serotype A Pasteurellamultocida

Cheng

et al. 2022

Genes

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Pasteurella multocida (P. multocida) is an opportunistic pathogen that is common in livestock and poultry and leads to massive economic losses in the animal husbandry sector. In this study, we challenged mice with P. multocida strain HN02 by intraperitoneal injection and collected spleens to measure bacterial loads. We also performed histopathological analysis by hematoxylin and eosin (H&E) staining. Then we used RNA-sequencing (RNA-seq) to detect the mRNA expression levels in the mouse spleen and quantitative real-time PCR (qRT-PCR) to verify the sequencing data. Finally, we examined the effect of HN02 on anti-inflammatory cytokine interleukin-10 (IL-10) protein expression in the spleen through immunohistochemical analysis. The results showed that compared to those in the control group, the mouse spleens in the challenge group had lesions, and the average bacteria loads was (3.07 ± 1.09) × 106 CFU (colony-forming unit)/g. The RNA-seq results determined 3653 differentially expressed genes (DEGs), and the qRT-PCR analysis revealed immune-related genes consistent with the expression trend in the sequencing data. The number and area of IL-10 positive cells substantially increased to resist inflammation in the challenge group. In conclusion, we analyzed the spleens of mice infected with P. multocida from multiple perspectives, and our findings lay a foundation for subsequent studies on the mechanism of pathogen-host interactions.

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Section: Discussionsupporting

confidence: 91%

Up-Regulation of Interleukin-10 in Splenic Immune Response Induced by Serotype A Pasteurellamultocida

Cheng

et al. 2022

Genes

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“…For the RNA−seq experiment, the cells were infected for 6 h with MOI of 100. The reason is that compared with the transcriptome data of the previous P. multocida −infected cells for 4 h with MOI of 100 [ 9 ], it was determined that there were almost no differentially peak-related genes gained from ATAC−seq overlapped with the differentially expressed genes gained from RNA−seq. We speculated that it may take a certain time for gene expression to be transcribed from DNA to RNA.…”

Section: Discussionmentioning

confidence: 99%

“…After the quality detection, the RNA was reverse transcribed and used for cDNA library construction. Sequencing and data analysis were performed as described elsewhere [ 9 , 10 ]. Briefly, the transcriptome data were assembled using StringTie and compared with the reference genome using Hisat.…”

Section: Methodsmentioning

confidence: 99%

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Profiling Chromatin Accessibility Responses in Goat Bronchial Epithelial Cells Infected with Pasteurella multocida

Chen

Zhang

et al. 2023

IJMS

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Pasteurella multocida can cause goat hemorrhagic sepsis and endemic pneumonia. Respiratory epithelial cells are the first line of defense in the lungs during P. multocida infection. These cells act as a mechanical barrier and activate immune response to protect against invading pathogenic microorganisms. Upon infection, P. multocida adheres to the cells and causes changes in cell morphology and transcriptome. ATAC-seq was conducted to determine the changes in the chromatin open region of P. multocida-infected goat bronchial epithelial cells based on transcriptional regulation. A total of 13,079 and 28,722 peaks were identified in the control (CK) and treatment (T) groups (P. multocida infection group), respectively. The peaks significantly increased after P. multocida infection. The specific peaks for the CK and T groups were annotated to 545 and 6632 genes, respectively. KEGG pathway enrichment analysis revealed that the specific peak-related genes in the T group were enriched in immune reaction-related pathways, such as Fc gamma R-mediated phagocytosis, MAPK signaling pathway, bacterial invasion of epithelial cells, endocytosis, and autophagy pathways. Other cellular component pathways were also enriched, including the regulation of actin cytoskeleton, adherent junction, tight junction, and focal adhesion. The differential peaks between the two groups were subsequently analyzed. Compared to those in the CK group, 863 and 11 peaks were upregulated and downregulated, respectively, after the P. multocida infection. Fifty-six known transcription factor motifs were revealed in upregulated peaks in the P. multocida-infected group. By integrating ATAC-seq and RNA-seq, some candidate genes (SETBP1, RASGEF1B, CREB5, IRF5, TNF, CD70) that might be involved in the goat bronchial epithelial cell immune reaction to P. multocida infection were identified. Overall, P. multocida infection changed the structure of the cell and caused chromatin open regions to be upregulated. In addition, P. multocida infection actively mobilized the host immune response with the inflammatory phenotype. The findings provide valuable information for understanding the regulatory mechanisms of P. multocida-infected goat bronchial epithelial cells.

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“…The P. multocida strain of serotype D is usually associated with HS and pneumonia [ 7 ]. Our previous study demonstrated that P. multocida strains of serotypes A (Pm HN02) and D (Pm HN01) led to different cellular morphologies in goat bronchial epithelial cells [ 8 ]. Whether the host immune responses induced by these two strains are different has not been reported.…”

Section: Introductionmentioning

confidence: 99%

Activation of MyD88-Dependent TLR Signaling Modulates Immune Response of the Mouse Heart during Pasteurella multocida Infection

Jiang

et al. 2023

Microorganisms

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Pasteurella multocida (P. multocida) is an important zoonotic pathogen. In addition to lung lesions, necropsies have revealed macroscopic lesions in the heart in clinical cases. However, most previous studies focused on lung lesions while ignoring heart lesions. Therefore, to investigate the immune response of the P. multocida-infected heart, two murine infection models were established by using P. multocida serotype A (Pm HN02) and D (Pm HN01) strains. Histopathological examination revealed heterogeneous inflammatory responses, including immune cell infiltration in the epicardial and myocardial areas of the heart. Transcriptome sequencing was performed on infected cardiac tissues. To explore the traits of immune responses, we performed the functional enrichment analysis of differentially expressed genes, gene set enrichment analysis and gene set variation analysis. The results showed that the innate immune pathways were significantly regulated in both groups, including the NOD-like receptor signaling pathway, the complement and coagulation cascade and cytokine–cytokine receptor interaction. The Toll-like receptor signaling pathway was only significantly activated in the Pm HN02 group. For the Pm HN02 group, immunohistochemistry analysis further verified the significant upregulation of the hub component MyD88 at the protein level. In conclusion, this study reveals critical pathways for host heart recognition and defense against P. multocida serotypes A and D. Moreover, MyD88 was upregulated by P. multocida serotype A in the heart, providing a theoretical basis for future prevention, diagnosis and treatment research.

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The mRNA and miRNA profiles of goat bronchial epithelial cells stimulated by Pasteurella multocida strains of serotype A and D

Cited by 5 publications

References 50 publications

Up-Regulation of Interleukin-10 in Splenic Immune Response Induced by Serotype A Pasteurellamultocida

Up-Regulation of Interleukin-10 in Splenic Immune Response Induced by Serotype A Pasteurellamultocida

Profiling Chromatin Accessibility Responses in Goat Bronchial Epithelial Cells Infected with Pasteurella multocida

Activation of MyD88-Dependent TLR Signaling Modulates Immune Response of the Mouse Heart during Pasteurella multocida Infection

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