The mouse embryo culture system: improving the sensitivity for use as a quality control assay for human in vitro fertilization

Fleetham, Judy; Pattinson, H. Anthony; Mortimer, David

doi:10.1016/s0015-0282(16)55638-6

Cited by 32 publications

(17 citation statements)

References 12 publications

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“…The sensitivity of the 1-cell MEA for CH in oil doubled when embryos were cultured individually as opposed to when cultured as a group. The sensitivity of the MEA increases when the zona pellucida is removed [8,24]. However, since zona-free embryos were cultured individually, further studies are needed to determine if the improved sensitivity was due to zona removal or culture density.…”

Section: Discussionmentioning

confidence: 99%

“…Although mouse embryo assays screen for toxins by simulating human embryo culture conditions the lack of standardization leads to variability in results [6]. Culture techniques have been employed to improve the sensitivity of the MEA, such as culturing fresh vs frozen embryos, using 1-cell vs 2-cell embryos or removing the zona pellucida prior to culture [7][8][9]. The 1-cell MEA, when compared to the 2-cell MEA, is ten times more sensitive for the toxin, Cidex, a common surgical sterilizing solution [7,10].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Peroxides in mineral oil used for in vitro fertilization: defining limits of standard quality control assays

Hughes¹,

Morbeck

Hudson³

et al. 2010

J Assist Reprod Genet

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Purpose To determine the relative sensitivities of the 1 and 2-cell mouse embryo assays (MEA) and the human sperm motility assay (HSMA) for peroxides in mineral oil. The effect of peroxide on blastocyst cell number and apoptosis was also studied. Methods One and two-cell MEA and HSMA were performed using mineral oil containing cumene hydroperoxide (CH).Results The 1-cell MEA was twice as sensitive as the 2-cell MEA and 20-times more sensitive than the HSMA for CH in mineral oil. The sensitivity of the 1-cell MEA doubled when embryos were cultured individually versus group culture. CH decreased blastocyst cell number in a dose dependent manner.Conclusions Individually cultured 1-cell embryos had the highest sensitivity for peroxides in mineral oil. Current quality control assays, including group cultured murine embryos and human sperm motility, have limited sensitivity for peroxides in mineral oil and may not detect levels of peroxides that cause sub-lethal cellular damage.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Peroxides in mineral oil used for in vitro fertilization: defining limits of standard quality control assays

Hughes¹,

Morbeck

Hudson³

et al. 2010

J Assist Reprod Genet

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show abstract

“…The stage of embryo also impacts the outcome, with one-cell embryos being more sensitive than two-cell, four-cell or eight-cell embryos [51,53]. Embryos can be made even more sensitive to culture conditions when the zona pellucida is removed [50,54]. The type of media also influences the outcome of the assay [53,55].…”

Section: The Use Of Mouse Embryos In Quality Control Systemsmentioning

confidence: 99%

Virtues and limitations of the preimplantation mouse embryo as a model system

Taft

2008

Theriogenology

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The mouse is the most widely used model of preimplantation embryo development, but is it a good model? Its small size, prolificacy and ease of handling make the mouse a relatively low cost, readily available and attractive alternative when embryos from other species are difficult or expensive to obtain. However, the real power of the mouse as a model lies in mouse genetics. The development of inbred mouse strains facilitated gene discovery as well as our understanding of gene function and regulation while the development of tools to introduce precise genetic modifications uniquely positioned the mouse as a powerful model system for uncovering gene function. However, all models have limitations; the small size of the mouse limits tissue availability and manipulations that can be performed and differences in physiology among species may make it inappropriate to extrapolate from the mouse to other species. Thus, rather than extrapolating directly from the mouse to other species, it may be more useful to use the mouse as a model system for developing and refining hypotheses to be tested directly in species of interest. In this brief review, the value of the preimplantation mouse embryo as a model is considered, both as a model for other species and as a model for the mouse, as understanding the virtues and limitations of the mouse as a model system is essential to its appropriate use.

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“…CE marking is accompanied by full disclosure of the medium's precise composition, an assessment of its quality, post-market surveillance, systems for reporting and examining adverse incidents, and traceability (21). On the other hand, MEA marking is indicative of successful testing for ≥80% of mouse embryo development to blastocyst stage (22,23) and has been widely criticized as it basically only reassures against toxicity issues rather than compatibility with human embryo development (21,(24)(25)(26). However, the actual number of embryos is not reported which may raise an issue regarding the validity of the results reported.…”

Section: Abstract There Are Numerous Reports On Embryo Culture Mediamentioning

confidence: 99%

Considerations Regarding Embryo Culture Conditions: From Media to Epigenetics

Simopoulou

Sfakianoudis²,

Rapani

et al. 2018

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The mouse embryo culture system: improving the sensitivity for use as a quality control assay for human in vitro fertilization

Cited by 32 publications

References 12 publications

Peroxides in mineral oil used for in vitro fertilization: defining limits of standard quality control assays

Peroxides in mineral oil used for in vitro fertilization: defining limits of standard quality control assays

Virtues and limitations of the preimplantation mouse embryo as a model system

Considerations Regarding Embryo Culture Conditions: From Media to Epigenetics

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