The motility of Entamoeba histolytica: finding ways to understand intestinal amoebiasis

Aguilar‐Rojas, Arturo; Olivo‐Marin, Jean‐Christophe; Guillén, Nancy

doi:10.1016/j.mib.2016.07.016

Cited by 29 publications

(27 citation statements)

References 42 publications

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“…ECM components have been used to coat surfaces where trophozoites are grown, for example collagen and FN (Ramírez-Tapia et al, 2015; Talamás-Lara et al, 2015), and these interaction trophozoite-substrate leads to a response as the differential expression of proteins, protease secretion (Piña-Vázquez et al, 2012), and cytoskeletal rearrangements in which actin polymerization is actively involved (Sengupta et al, 2009; Javier-Reyna et al, 2012; Starke et al, 2014; Aguilar-Rojas et al, 2016). EMC components (such as FN) have been used to generate micropatterns in order to stimulate eukaryotic cells to migrate, produce abundant and different membrane protrusions (Alamdari et al, 2013; Starke et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…During intestinal infection, trophozoites disrupt, and degrade intestinal mucosa barrier, and can also trogocyte mucosal epithelial cells and promote cell death by multiple cytotoxic mechanisms (Ralston et al, 2014; Begum et al, 2015). In the course of invasion, trophozoites interact with extracellular matrix components (ECM) such as fibronectin (FN), laminin and collagen (Solaymani-Mohammadi and Petri, 2008), which in vitro induce actin cytoskeleton remodeling that is involved in adhesion, migration (Sengupta et al, 2009; Javier-Reyna et al, 2012) and motility (Aguilar-Rojas et al, 2016). …”

Section: Introductionmentioning

confidence: 99%

“…Migration, associated with changes in trophozoite morphology, is stimulated by different factors such as the need for nutrients or the chemoattractant environment, which induces the characteristic amoeboid movement of trophozoites (Aguilar-Rojas et al, 2016). In two-dimensional cell culture surfaces, the migration, and motility of trophozoite usually show a fast generation of blebs and pseudopodia protrusions at the leading edge (Maugis et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…In two-dimensional cell culture surfaces, the migration, and motility of trophozoite usually show a fast generation of blebs and pseudopodia protrusions at the leading edge (Maugis et al, 2010). Occasionally retracting uropod at the rear end and short or large filopodia are produced by trophozoites in normal culture (González-Robles and Martínez-Palomo, 1983; Marquay Markiewicz et al, 2011), therefore these structures are generally not mentioned in the description of the mobility of the parasite (Aguilar-Rojas et al, 2016). However, filopodia of 1-6 micrometers extending between the trophozoites and MDKC or Caco-2 cell monolayers were reported (Li et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

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Influence of Micropatterned Grill Lines on Entamoeba histolytica Trophozoites Morphology and Migration

Sierra-López

Baylón-Pacheco

Espíritu-Gordillo

et al. 2018

Front. Cell. Infect. Microbiol.

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Entamoeba histolytica, the causal agent of human amoebiasis, has two morphologically different phases: a resistant cyst and a trophozoite responsible for the invasion of the host tissues such as the colonic mucosa and the intestinal epithelium. During in vitro migration, trophozoites usually produce protuberances such as pseudopods and rarely filopodia, structures that have been observed in the interaction of trophozoites with human colonic epithelial tissue. To study the different membrane projections produced by the trophozoites, including pseudopods, filopodia, uropods, blebs, and others, we designed an induction system using erythrocyte extract or fibronectin (FN) in micropatterned grill lines (each micro-line containing multiple micro-portions of FN or erythrocyte extract) on which the trophozoites were placed in culture for migration assays. Using light, confocal, and scanning electron microscopy, we established that E. histolytica trophozoites frequently produce short and long filopodia, large retractile uropods in the rear, pseudopods, blebs, and others structures, also showing continuous migration periods. The present study provides a simple migration method to induce trophozoites to generate abundant membrane protrusion structures that are rarely obtained in normal or induced cultures, such as long filopodia; this method will allow a–better understanding of the interactions of trophozoites with FN and cell debris. E. histolytica trophozoites motility plays an important role in invasive amoebiasis. It has been proposed that both physical forces and chemical signals are involved in the trophozoite motility and migration. However, the in vivo molecules that drive the chemotactic migration remain to be determined. We propose the present assay to study host molecules that guide chemotactic behavior because the method is highly reproducible, and a live image of cell movement and migration can be quantified.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Influence of Micropatterned Grill Lines on Entamoeba histolytica Trophozoites Morphology and Migration

Sierra-López

Baylón-Pacheco

Espíritu-Gordillo

et al. 2018

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

show abstract

“…The infection caused by E. histolytica is the third major parasitic disease responsible for approximately 10 million deaths per year [4,5]. According to a recent research report, each year 50 million new cases of invasive amoebiasis occur globally [3,6].…”

Section: Introductionmentioning

confidence: 99%