The Morphology of Vesicular Stomatitis Virus (Indiana C) Derived from Chick Embryos or Cultures of BHK21/13 Cells

Bradish, C. J.; Kirkham, Jennifer

doi:10.1099/00221287-44-3-359

Cited by 18 publications

(9 citation statements)

References 10 publications

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“…The sedimentation coefficient obtained with the wildtype T particle is in agreement with the original estimate (330S) reported by Bradish, Brooksby, and Dillon (2) for the T particle of this strain. The physical properties of the T particles of the wild type were not affected by the temperature of multiplication (31, 37, or 39 C) and are similar to those reported in the literature (2,3,5,6,(8)(9)(10)(11)). Furthermore, identical results were obtained with three other preparations of wildtype virus derived from single-plaque isolates on different occasions.…”

Section: Physical Properties Of Vsv T Particles and Theirsupporting

confidence: 85%

“…The observation that the nucleic acid of defective particles is smaller than the virion nucleic acid would be consistent with this interpretation. Although many animal viruses produce defective particles, the T (or truncated) particles of vesicular stomatitis virus (VSV) have been extensively studied with respect to their biological and physical properties (2,3,5,6,(8)(9)(10)(11).…”

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confidence: 99%

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Defective Particles in BHK Cells Infected with Temperature-Sensitive Mutants of Vesicular Stomatitis Virus

Reichmann

Pringle

Follett

1971

J Virol

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Defective particles were the major product after undiluted passage of certain temperature-sensitive ( ts ) mutants of the Indiana C strain of vesicular stomatitis virus in BHK-21 cells at the permissive temperature (31 C). Essentially homogeneous preparations of defective particles were obtained with the wild-type and individual ts mutants. The defective particles associated with some of the ts mutants, however, were morphologically and physically distinguishable from wild type and from each other. All varieties of defective particle interfered with the multiplication of mutant and wild-type virus at the permissive temperature at early times of infection but failed to complement virions of different complementation groups at the restrictive temperature (39 C) at any time during infection.

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Section: Physical Properties Of Vsv T Particles and Theirsupporting

confidence: 85%

mentioning

confidence: 99%

Defective Particles in BHK Cells Infected with Temperature-Sensitive Mutants of Vesicular Stomatitis Virus

Reichmann

Pringle

Follett

1971

J Virol

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“…Assuming that 1 ID.,, corresponds to 10 physical particles (a rather conservative estimate), roughly 10,000 virus particles were pro duced per cell. This is probably as good as can be expected from a fully adapted virus-cell system and compares favorably with published data [2,3].…”

Section: Discussionsupporting

confidence: 77%

Growth of Vesicular Stomatitis Virus in Human Myeloblasts

U¹

1970

Pathobiology

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A strain of Vesicular Stomatitis virus, serotype Indiana (VSV), was compared after a single in vitro passage and after 16 in vitro passages in human white blood cells from patients suffering mostly from acute myeloid leukemia. The in vitro growth characteristics of these two virus lines in white blood cells (predominantly myeloblasts) from a patient with acute myeloid leukemia were measured, starting with low multiplicities of infection. No differences were seen in the following parameters: Length of latency phase, growth rate, final titer, thermal inactivation rate, plaque size, titer difference in chick embryo vs. human diploid cells, and extent of neutralization by a standard rabbit antiserum. Infectivity titrations in chick embryo cells gave consistently about ten times higher values than the same titrations done in human diploid cells, strain Wi38. More than 1,000 infectious units of VSV (as measured in chick embryo cells) were produced per myeloblast within 44 h. It was concluded that a single passage was sufficient to fully adapt VSV to human myeloblasts.

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“…VSV is a widely studied virus that has a well-defined structure. The virus causes periodic outbreaks of disease in cattle, horses, and swine in Central and North America. − Its main structural features have been established by electron microscopy. ,− The infectious VS virion is bullet-shaped and consists of an internal helical structure (RNA and nucleocapsid protein) surrounded by a protein matrix and a phospholipid bilayer studded with fine projections (VSV-G protein). , The virion is approximately 173 nm (range 125−205 nm) in length and 75 nm (range 45−85 nm) in diameter. , A schematic illustration of the infectious VS virion is shown in Figure . Electrokinetic studies have determined that VSV has an isoelectric range from pH 4.7 to pH 7.0 …”

Section: Introductionmentioning

confidence: 99%

Orientational Behavior of Thermotropic Liquid Crystals on Surfaces Presenting Electrostatically Bound Vesicular Stomatitis Virus

et al. 2004

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We report the orientational behavior of nematic phases of 4-cyano-4'-pentylbiphenyl (5CB) on cationic, anionic, and nonionic surfaces before and after contact of these surfaces with solutions containing the negatively charged vesicular stomatitis virus (VSV). The surfaces were prepared on evaporated films of gold by either adsorption of poly-L-lysine (cationic) or formation of self-assembled monolayers (SAMs) from HS(CH2)2SO3- (anionic) or HS(CH2)11(OCH2CH2)4OH (nonionic). Prior to treatment with virus, we measured the initial orientation of 5CB (delta epsilon = epsilon(parallel) - epsilon(perpendicular) > 0) to be parallel to the cationic surfaces (planar anchoring) but perpendicular (homeotropic) after equilibration for 5 days. A similar transition from planar to homeotropic orientation of 5CB was observed on the anionic surfaces. Only planar orientations of 5CB were observed on the nonionic surfaces. Because N-(4-methoxybenzylidene)-4-butylaniline (MBBA, delta epsilon = epsilon(parallel) - epsilon(perpendicular) < 0) exhibited planar alignment on all surfaces, the time-dependent alignment of 5CB on the ionic surfaces is consistent with a dipolar coupling between the 5CB and electrical double layers formed at the ionic interfaces. Treatment ofpoly-L-lysine-coated gold films (cationic) with purified solutions of VSV containing 10(8)-10(10) plaque-forming units per milliliter (pfu/mL) led to the homeotropic alignment of 5CB immediately after contact of 5CB with the surface. In contrast, treatment of anionic surfaces and nonionic surfaces with solutions of VSV containing approximately 10(10) pfu/mL did not cause immediate homeotropic alignment of 5CB. These results and others suggest that homeotropic alignment of 5CB on cationic surfaces treated with VSV of titer > or = 10(8) pfu/mL reflects the presence of virus electrostatically bound to these surfaces.

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The Morphology of Vesicular Stomatitis Virus (Indiana C) Derived from Chick Embryos or Cultures of BHK21/13 Cells

Cited by 18 publications

References 10 publications

Defective Particles in BHK Cells Infected with Temperature-Sensitive Mutants of Vesicular Stomatitis Virus

Defective Particles in BHK Cells Infected with Temperature-Sensitive Mutants of Vesicular Stomatitis Virus

Growth of Vesicular Stomatitis Virus in Human Myeloblasts

Orientational Behavior of Thermotropic Liquid Crystals on Surfaces Presenting Electrostatically Bound Vesicular Stomatitis Virus

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