The morphological and functional observation of the gap junction proteins in the oviduct epithelia in young and adult hamsters

Yan, Jun; Akutsu, Hitomi; Satoh, Yóichi

doi:10.2535/ofaj.88.57

Cited by 4 publications

(2 citation statements)

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“…Advanced maternal age is associated with a decline in oocyte quality, reduced fertility, and adverse pregnancy outcomes (Malhi et al, ; Nelson et al, ); the latter two phenotypes may be linked to changes in oviduct function. Indeed, Yan et al () previously reported morphological and functional differences in gap junctions of oviduct epithelia in young and adult hamsters. In the present study, we hypothesized that bovine OECs also exhibit age‐dependent changes, and thus investigated if this system could be used to understand oviduct senescence.…”

Section: Introductionmentioning

confidence: 98%

Age-dependent changes in inflammation and extracellular matrix in bovine oviduct epithelial cells during the post-ovulatory phase

et al. 2016

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The mammalian oviduct is an essential site for sperm storage, the transport of gametes, fertilization, and embryo development-functions that are aided by cytokines secreted from oviduct epithelial cells (OECs). Aging leads to cellular and organ dysfunction, with infertility associated with advanced maternal age. Few studies have investigated age-dependent changes in the oviduct as a possible cause of infertility, so we compared OECs from young (30-50 months) versus aged (more than 120 months) cattle. Next-generation sequencing was first used to identify age-related differences in gene expression. Several proinflammatory-related genes (including IL1B, IL1A, IL17C, IL8, S100A8, S100A9, and TNFA) were activated in OECs from aged (more than 120 months) compare to young (30-50 months) individuals, whereas genes associated with extracellular matrix-related factors (COLs, POSTN, BGN, and LUM) were down-regulation in aged OECs. Indeed, IL1 B and IL8 abundance was higher in aged OECs than in young OECs. Young OECs also tended to proliferate faster, and the revolution frequency of young, ciliated OECs was higher than that of their aged counterparts. In contrast, aged OECs possessed more F-actin, an actin cytoskeleton marker associated with reduced elasticity, and contained high levels of reactive oxygen species, which are mediators of inflammation and senescence. These different functional characteristics of bovine OECs during the post-ovulatory phase support the emerging concept of "inflammaging," that is, age-dependent inflammation. Mol. Reprod. Dev. 83: 815-826, 2016 © 2016 Wiley Periodicals, Inc.

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Section: Introductionmentioning

confidence: 98%

Age-dependent changes in inflammation and extracellular matrix in bovine oviduct epithelial cells during the post-ovulatory phase

et al. 2016

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“…Limited information is available regarding the age-dependent changes influencing the functioning of the oviduct. Yan et al [ 29 ] have reported on the morphological and functional differences in the gap junctions of oviduct epithelia in young and adult hamsters. We have previously shown that the age-dependent changes in bovine OECs are mediated in part by pro-inflammatory signaling and extracellular matrix (ECM)-related components [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of aging on the female reproductive function

Shirasuna

Iwata

2017

Contracept Reprod Med

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Aging is a complex biological process that involves the accrual of bodily changes over a long life span. In humans, advanced maternal age is associated with infertility and adverse pregnancy complications. Cellular and organic senescence is hypothesized to contribute to the age-related decline in reproductive function. Accumulating evidence suggests that immune cells play pivotal roles in physiological reproductive function and pregnancy. The concept of “inflammaging” has recently emerged- an age-dependent, low-grade, chronic, and systemic inflammatory state induced by the senescence-associated secretory phenotype (SASP), which is produced by the innate immune, parenchymal, and nonparenchymal cells within the organs. In the present review, we discuss how cellular senescence and inflammaging accelerate reproductive failure in women by promoting SASP and immune-senescence during the establishment of pregnancy. In addition, we discuss the role of immune cells and their senescence in reproductive function, particularly in the ovaries (the corpus luteum), oviduct, and uterus.

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Transcriptome analysis and identification of age-associated fertility decreased genes in hen uterovaginal junction

Yang

et al. 2021

Poultry Science

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Avian sperm storage tubules ( SSTs ), which are located in the uterovaginal junction ( UVJ ) of the oviduct, are primary sperm storage sites after mating or artificial insemination. The mechanism underlying reduced sperm storage efficiency of SSTs which is highly correlated with decreased fertility rates in aged laying breeders remains largely unclear. Here, comparative transcriptomic analysis between the aged and young White Leghorn hens (120 vs. 30 wk) was applied to identify gene expression changes of UVJs containing SSTs. Bioinformatics analysis revealed 567 upregulated and 1998 downregulated differentially expressed genes. Gene ontology analysis was highly enriched in terms of immune system, cell adhesion, and cytoskeleton proteins. Kyoto Encyclopedia of Genes and Genomes analysis revealed 5 significant ( P < 0.05) pathways including inositol phosphate and glycerophospholipid metabolism. β-Galactosidase staining of chicken UVJ sections suggested increased cell senescence via aging. Oil Red O staining and immunohistochemistry detection of ADFP both confirmed distribution of lipid droplets in SST cells with increased intensity in aged breeders. The lipid synthesis and metabolism-related genes represented by TFAP2 and PLD1 were differentially expressed in aged laying breeders. The upregulation of IL15 and downregulation of a large number of immune-related genes in aged breeders indicate altered immune homeostasis in UVJs and SSTs. The increased accumulation of lipids, and altered immunity homeostasis, combined with other factors ( TJP1 , MYL9 , AFDN , and RPL13 , etc.) are potentially dominant effectors to decrease the sperm storage efficiency and egg fertility in aged laying breeders.

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The morphological and functional observation of the gap junction proteins in the oviduct epithelia in young and adult hamsters

Cited by 4 publications

References 34 publications

Age-dependent changes in inflammation and extracellular matrix in bovine oviduct epithelial cells during the post-ovulatory phase

Age-dependent changes in inflammation and extracellular matrix in bovine oviduct epithelial cells during the post-ovulatory phase

Effect of aging on the female reproductive function

Transcriptome analysis and identification of age-associated fertility decreased genes in hen uterovaginal junction

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