The Monoxenic Culture of Arbuscular Mycorrhizal Fungi as a Tool for Systematics and Biodiversity

Development of an autotrophic culture system for the in vitro mycorrhization of potato plantlets

“…Therefore, the strict one-to-one organism interaction could be disturbed. In the bipartite culture system on polyurethane foam [14] the development of the extraradical mycelium as well as its micro-morphology, i.e., the architecture of the colony [19] is difficult to investigate non-destructively.…”

Continuous and long-term monoxenic culture of the arbuscular mycorrhizal fungus Gigaspora decipiens in root organ culture

“…However, these fungi have proven to be more difficult than other AM species with small-diameter and thin-walled spores to sub-culture and propagate, for long periods of time under monoxenic conditions (Dalpe et al 2005). This could be related to the different life strategies of each AM fungal species.…”

A simple method to obtain endophytic microorganisms from field-collected roots

“…No regrowth of AMF from roots of T. repens and S. halepense was observed. This finding shows that roots of different plant species with the same origin not always generate a similar number of fungal isolations and that not all root-inhabiting AMF are easily established in monoxenic cultures (Dalpe et al, 2005). Since a high number of root segments with endophytic bacteria were found using hypochlorite as the disinfectant solution, we consider that this method is a powerful means for the isolation of endophytic bacteria.…”

“…1F and G) were growing together after the isolation; therefore, this system could allow us to study the interactive relationships between both endophytic microorganisms, where species interaction can be isolated and controlled. For example, adding antibiotics, as it was mentioned above, in the root disinfection process and the Gel-Gro medium increased the number of root fragments with AMF hyphae regrowth and other fungi, most likely because of the antagonistic interactions between bacteria and fungi (Krechel et al, 2002;Cao et al, 2005;Dalpe et al, 2005).…”