Continuous and long-term monoxenic culture of the arbuscular mycorrhizal fungus Gigaspora decipiens in root organ culture

Bidondo, Laura Fernández; Pérgola, M.; Silvani, Vanesa; Colombo, Roxana; Bompadre, Josefina; Godeas, Alicia Margarita

doi:10.1016/j.funbio.2012.04.007

Cited by 8 publications

(6 citation statements)

References 25 publications

(25 reference statements)

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“…Although the application of AMF is encouraged to improve the sustainability of organic agriculture, the amount of AMF inoculum required for large-scale application cannot be achieved in a cost-effective manner using current mass-production techniques [ 9 ]. For instance, aeroponic [ 10 ] and root organ culture [ 11 ] methods are limited to the laboratory scale and are costly for AMF mass production.…”

Section: Introductionmentioning

confidence: 99%

Effects of Long-Term Subcultured Arbuscular Mycorrhizal Fungi on Red Pepper Plant Growth and Soil Glomalin Content

Selvakumar

Lee

et al. 2018

Mycobiology

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Arbuscular mycorrhizal fungi (AMF) are well-known for their ability to improve plant growth and help plants withstand abiotic stress conditions. Unlike other fungi and bacteria, AMF cannot be stored, as they are obligate biotrophs. Long-term preservation of AMF spores is challenging and may lead to the loss of viability and efficiency. This study aimed to understand the effect of prolonged subculture of AMF species on the growth and glomalin-related soil protein (GRSP) from red pepper (Capsicum annuum L.). AMF spores were mass-produced using different techniques and subcultured in pots with sorghum sudangrass as the host plant for 3 years. Experimental soil samples were collected from natural grassland. Five different AMF inocula were used in triplicate as treatments. After 70 days of growth, red pepper plants were harvested and plant dry weight, plant nutrient content, mycorrhizal colonization, AMF spore count, and soil glomalin content were determined. AMF-treated plants displayed higher dry weight than controls, with only fruit dry weight being significantly different. Similarly, significant differences in phosphorous and potassium contents of the above-ground plant parts were observed between mycorrhizal and control treatments. In addition, soil GRSP content was significantly higher in plants inoculated with Rhizophagus sp. and Gigaspora margarita. The increased plant growth and GRSP content suggest that AMF can be maintained for 3 years without losing their efficiency if subcultured regularly with different symbiotic host plants.

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Section: Introductionmentioning

confidence: 99%

Effects of Long-Term Subcultured Arbuscular Mycorrhizal Fungi on Red Pepper Plant Growth and Soil Glomalin Content

Selvakumar

Lee

et al. 2018

Mycobiology

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“…Carrot is one of the most suitable and well known model plant species for hairy root production (Bidondo et al, 2012).In this present study, it was observed that inoculation of carrot discs with 48 hours old A. rhizogenes (MTCC-532) culture and incubated in darkness at 27 o C provided a suitable condition for bacterial strains to insert their copies of Ri t-DNA. These findings were pointed out by Mugnier and Mosse, (1987) who observed that transformation efficiency is highly dependent on the bacterial strain used, which is related to the type of vir plasmid, binary plasmid or bacterial chromosomal background as a factor influencing hairy root initiation.…”

Section: Discussionmentioning

confidence: 70%

Establishing monoxenic culture of arbuscular mycorrhizal fungus Glomus intraradices through root organ culture

Srinivasan

Kumar

Kumutha

et al. 2014

JANS

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Arbuscular mycorrhizal fungi are soil fungi distributed worldwide, forming symbiosis with most of the vascular plants for their growth and survival, which is used for sustainable agriculture and ecosystem management. This study investigated the establishment of monoxenic cultures of Glomus intraradices in association with transformed carrot hairy root. The G.intraradices spores were isolated from sugarcane rhizosphere by wet sieving and decanting technique and propagated in open pot culture. Transformation in to carrot hairy root was done using Agrobacterium rhizogenes. Surface sterilization of G.intraradices spores co-cultured with transformed carrot hairy root in Modified Strulla and Romand (MSR) medium was found the host root growth as well as for germination AM spores. After three months of incubation in dark condition, significant production of extensive hyphal growth on MSR medium and an average of 8500-9000 spores per petri dish was observed. The in vitro inoculum exhibited higher potential of root colonization due to numerous intraradices mycelium with extensive spore load. The produced monoxenic inoculum can be used in place of traditional system where it has a advantage of producing contaminant free propagulas. Thus the monoxenic culture system, a powerful tool, of AM sporulation, can be used for the mass production of monoxenic inoculum of AM fungi besides studying its biology.

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“…The results of the two different plants confirms the difference between the several potential microbes tested in this process (Figure 2). Plants absorb phosphorus (P) and nitrogen (N) from the soil through root transporters, but the bioavailable forms of P and N are limited in rhizospheres [41]. Bacillus sp.…”

Section: Discussionmentioning

confidence: 99%

The Efficacy of Functional Composts Manufactured Using Spent Coffee Ground, Rice Bran, Biochar, and Functional Microorganisms

2021

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Spent coffee grounds (SCGs), one of the world’s most discarded wastes, may be an excellent resource as an organic fertilizer because of its richness in nutrients. The objective of this study was to develop a quality functional compost using SCGs, rice bran, biochar, SCG extract, and functional microbes (plant growth promoting and plant pathogen-suppression bacteria), and then to test their functional efficacy for a potential commercial application. Essentially, two types of representative composts (Tr_1 and Tr_5 on the laboratory and pilot scale, respectively) were developed and passed all the official commercial quality standards. For pilot-scale composting, populations of Halotalea_uc, Corynebacterium nuruki, and Lactobacillus acidipiscis increased by augmentation of the composting microbes (MA-1) and the functional microbes (Bacillus cereus SB-3, Bacillus toyonensis SB-4, and Streptomyces sasae St-3). The higher total flavonoid content (11% increase compared to control) of pepper leaves in PT-1 and the higher TEAC in PT-1 (36.2%) and PT-2 (32.5%) proved the efficacy of the functional composts bioaugmented with the functional microbes. The seedling growth of radish seeds treated with Streptomyces sasae St-3 as a biocontrol agent significantly increased despite the presence of the pathogen Fusarium oxysporum f. sp. lactucae. The total phenol content and TEAC in pepper plant leaves were significantly higher in Tr_5 than in the control (Tr_4), whereas there were no differences in Tr_4 and Tr_5 infested with the fungal pathogens, indicating that SB-3, SB-4, and St-3 cultures amended within the compost (Tr_5) may facilitate the production of the antioxidants in the absence of the pathogens. However, a significant reduction in the antioxidants (total phenolic content and TEAC) was observed in the pepper plants whose roots were infected with the pathogens, indicating that the pathogens could neutralize functionalities of the functional microbes. It was concluded that the enhancement of functional microbes in the compost would aid in the biological control of pathogens in the soil environment. Further functional compost studies are necessary in terms of mechanisms of plant growth-promotion, mechanisms of pathogen suppression by the actinobacterial biocontrol agents, and interactions between the two mechanisms, as well as quality enhancement of the composts.

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Continuous and long-term monoxenic culture of the arbuscular mycorrhizal fungus Gigaspora decipiens in root organ culture

Cited by 8 publications

References 25 publications

Effects of Long-Term Subcultured Arbuscular Mycorrhizal Fungi on Red Pepper Plant Growth and Soil Glomalin Content

Effects of Long-Term Subcultured Arbuscular Mycorrhizal Fungi on Red Pepper Plant Growth and Soil Glomalin Content

Establishing monoxenic culture of arbuscular mycorrhizal fungus Glomus intraradices through root organ culture

The Efficacy of Functional Composts Manufactured Using Spent Coffee Ground, Rice Bran, Biochar, and Functional Microorganisms

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