The Molecular Technology Revolution and Bacterial Identification: Unexpected Consequences for Clinical Microbiologists

Janda, J. Michael

doi:10.1016/j.clinmicnews.2023.03.001

Cited by 2 publications

(1 citation statement)

References 35 publications

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“…Molecular techniques have enabled researchers to uncover previously unrecognized microbial diversity. These techniques have facilitated the identification and characterization of bacterial species that were difficult to study using traditional culture methods [5].…”

Section: Introductionmentioning

confidence: 99%

Sequencing And Phylogenetic Analysis of Microbiota Isolated from Different Types of Street Food Samples by using 16S rRNA Sequencing and Insilico Analysis

et al. 2023

IJFMR

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Street food is the one which is sold by vendors with a stall or maybe shop. Here using five different varieties of street food (Panipuri, Bhel, Momo, Dahibara, Chocolate), using different microbial techniques. Different microbes (Gram +ve and Gram -ve) have been observed, and using medicinal plants like (Neem, Tulsi, Parijat) antimicrobial testing has been done which shows the zone of inhibition and calculated result show the high amount of antimicrobial agent is Nyctanthes arbor-tristis (Parijat) with a zone of inhibition of 15 mm, 26 mm, 22 mm, 27 mm, 13 mm, etc. for 5 different samples respectively. Studies have come across a previously unidentified microbial variety through the use of molecular methods mainly 16S rRNA sequencing. The identification of novel taxa, a shortage of sequences in nucleotide databases, the existence of species with similar and/or identical 16S rRNA sequences, and nomenclature issues brought on by the assignment of multiple genomovars to a single species or complex are all challenges that must be overcome. Sequence data are analyzed using insilico method. To describe the connections between different bacterial strains, phylogenetic trees can be employed. This can detect strains that are very closely linked to one another and show which bacterial strains have been transmitted to whom.

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Section: Introductionmentioning

confidence: 99%

Sequencing And Phylogenetic Analysis of Microbiota Isolated from Different Types of Street Food Samples by using 16S rRNA Sequencing and Insilico Analysis

et al. 2023

IJFMR

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From Species to Genes: A New Diagnostic Paradigm

Fahy,

O’Connor,

Sleator

et al. 2024

Antibiotics

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Molecular diagnostics has the potential to revolutionise the field of clinical microbiology. Microbial identification and nomenclature have, for too long, been restricted to phenotypic characterisation. However, this species-level view fails to wholly account for genetic heterogeneity, a result of lateral gene transfer, mediated primarily by mobile genetic elements. This genetic promiscuity has helped to drive virulence development, stress adaptation, and antimicrobial resistance in several important bacterial pathogens, complicating their detection and frustrating our ability to control them. We argue that, as clinical microbiologists at the front line, we must embrace the molecular technologies that allow us to focus specifically on the genetic elements that cause disease rather than the bacterial species that express them. This review focuses on the evolution of microbial taxonomy since the introduction of molecular sequencing, the role of mobile genetic elements in antimicrobial resistance, the current and emerging assays in clinical laboratories, and the comparison of phenotypic versus genotypic analyses. In essence, it is time now to refocus from species to genes as part of a new diagnostic paradigm.

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The Molecular Technology Revolution and Bacterial Identification: Unexpected Consequences for Clinical Microbiologists

Cited by 2 publications

References 35 publications

Sequencing And Phylogenetic Analysis of Microbiota Isolated from Different Types of Street Food Samples by using 16S rRNA Sequencing and Insilico Analysis

Sequencing And Phylogenetic Analysis of Microbiota Isolated from Different Types of Street Food Samples by using 16S rRNA Sequencing and Insilico Analysis

From Species to Genes: A New Diagnostic Paradigm

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