Sequencing And Phylogenetic Analysis of Microbiota Isolated from Different Types of Street Food Samples by using 16S rRNA Sequencing and Insilico Analysis

Abstract

Street food is the one which is sold by vendors with a stall or maybe shop. Here using five different varieties of street food (Panipuri, Bhel, Momo, Dahibara, Chocolate), using different microbial techniques. Different microbes (Gram +ve and Gram -ve) have been observed, and using medicinal plants like (Neem, Tulsi, Parijat) antimicrobial testing has been done which shows the zone of inhibition and calculated result show the high amount of antimicrobial agent is Nyctanthes arbor-tristis (Parijat) with a zone of inhibition of 15 mm, 26 mm, 22 mm, 27 mm, 13 mm, etc. for 5 different samples respectively. Studies have come across a previously unidentified microbial variety through the use of molecular methods mainly 16S rRNA sequencing. The identification of novel taxa, a shortage of sequences in nucleotide databases, the existence of species with similar and/or identical 16S rRNA sequences, and nomenclature issues brought on by the assignment of multiple genomovars to a single species or complex are all challenges that must be overcome. Sequence data are analyzed using insilico method. To describe the connections between different bacterial strains, phylogenetic trees can be employed. This can detect strains that are very closely linked to one another and show which bacterial strains have been transmitted to whom.