The molecular study of microbial and functional diversity of resistant microbes in heavy metal contaminated soil

Njoku, Kelechi L.; Asunmo, Maryam O.; Ude, Emea O.; Adesuyi, Adeola Alex; Oyelami, Ayodeji O.

doi:10.1016/j.eti.2020.100606

Cited by 24 publications

(18 citation statements)

References 25 publications

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“…Even though sparingly investigated, fungal communities are consistently found to be more resilient than bacterial ones in HMs contaminated substrates [ 37 , 38 , 85 ]. Metal-resistant fungi, autochthonous to contaminated soils, show remarkable degrees of resistance towards HMs.…”

Section: Discussionmentioning

confidence: 99%

Heavy Metal-Resistant Filamentous Fungi as Potential Mercury Bioremediators

Văcar

Covaci

Chakraborty

et al. 2021

JoF

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Filamentous fungi native to heavy metals (HMs) contaminated sites have great potential for bioremediation, yet are still often underexploited. This research aimed to assess the HMs resistance and Hg remediation capacity of fungi isolated from the rhizosphere of plants resident on highly Hg-contaminated substrate. Analysis of Hg, Pb, Cu, Zn, and Cd concentrations by X-ray spectrometry generated the ecological risk of the rhizosphere soil. A total of 32 HM-resistant fungal isolates were molecularly identified. Their resistance spectrum for the investigated elements was characterized by tolerance indices (TIs) and minimum inhibitory concentrations (MICs). Clustering analysis of TIs was coupled with isolates’ phylogeny to evaluate HMs resistance patterns. The bioremediation potential of five isolates’ live biomasses, in 100 mg/L Hg2+ aqueous solution over 48 h at 120 r/min, was quantified by atomic absorption spectrometry. New species or genera that were previously unrelated to Hg-contaminated substrates were identified. Ascomycota representatives were common, diverse, and exhibited varied HMs resistance spectra, especially towards the elements with ecological risk, in contrast to Mucoromycota-recovered isolates. HMs resistance patterns were similar within phylogenetically related clades, although isolate specific resistance occurred. Cladosporium sp., Didymella glomerata, Fusarium oxysporum, Phoma costaricensis, and Sarocladium kiliense isolates displayed very high MIC (mg/L) for Hg (140–200), in addition to Pb (1568), Cu (381), Zn (2092–2353), or Cd (337). The Hg biosorption capacity of these highly Hg-resistant species ranged from 33.8 to 54.9 mg/g dry weight, with a removal capacity from 47% to 97%. Thus, the fungi identified herein showed great potential as bioremediators for highly Hg-contaminated aqueous substrates.

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Section: Discussionmentioning

confidence: 99%

Heavy Metal-Resistant Filamentous Fungi as Potential Mercury Bioremediators

Văcar

Covaci

Chakraborty

et al. 2021

JoF

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“…The works of Smarda et al [31] and Njoku et al [18] reported that GC-rich genes facilitate the response to environmental stress. In addition, it can also facilitate complex gene regulation.…”

Section: Discussionmentioning

confidence: 99%

“…Polymerase chain reaction (PCR) conditions were set on an initial denaturation temperature of 94 ˚C for 5 m, followed by 35 cycles of 30 s denaturation at 94 C, 30 s annealing of primer at 55 ˚C, 1½ minutes extension at 72 °C and a nal extension for 7 m at 72 °C [18].…”

Section: Molecular Characterization Of Isolates 261 Colony Dna Extrmentioning

confidence: 99%

Physiological and molecular characterization of active fungi in pesticides contaminated soils for degradation of glyphosate

Njoku¹,

Eludini²,

Adesuyi³

et al. 2020

Preprint

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Understanding the physiological and molecular characteristics of naturally occurring fungi in glyphosate pesticide-contaminated environment is crucial to managing its contamination. The study was aimed at isolating and characterizing soil fungi for their physiological roles towards glyphosate degradation. Pure cultures of fungi were isolated from soil contaminated with glyphosate at farms in Lagos, Nigeria. The cultures were grown on minimal salt agar media amended with glyphosate. The best isolates exhibiting good tolerance to the glyphosate were characterized using molecular techniques. The BLAST search indicated that the fungi belong to four Aspergillus species (Aspergillus flavus strain JN-YG-3-5, Aspergillus niger strain APBSDSF96, Aspergillus fumigatus strain FJAT-31052 and Aspergillus flavus strain APBSWTPF130, Trichoderma gamsii and Penicillium simplicissimum. The biodegradation study of the glyphosate by the selected fungi species showed the presence of Aminomethylphosphonic Acid (AMPA) except for Aspergillus fumigatus strain FJAT-31052. Annotation analysis of the partial gene sequence showed that the strains possess protein coding gene clusters for glyphosate utilization and other physiological activities. The GhostKOALA output confirmed that CYP2W1 gene (Cytochrome P450, fungi type) was present in Aspergillus fumigatus strain FJAT-31052 which was absent in the genome of other fungi. The physiological and molecular characteristics of Aspergillus fumigatus strain FJAT-31052 clearly show that this fungus is a useful organism for managing contamination by glyphosate pesticide.

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“…Aspergillus avus JN-YG-3-5 can be a particularly important tools for use in biotechnology because it yielded high pure DNA quantity and has a GC content similar to well-known GC in soil for active physiological functions. The works of Smarda et al [31] and Njoku et al [18] reported that GC-rich genes facilitate the response to environmental stress. In addition, it can also facilitate complex gene regulation.…”

Section: Discussionmentioning

confidence: 99%

Physiological and molecular characterization of active fungi in pesticides contaminated soils for degradation of glyphosate

Njoku

Eludini

Adesuyi

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Understanding the physiological and molecular characteristics of naturally occurring fungi in glyphosate pesticide contaminated environment is crucial to managing its contamination. The study was aimed at isolating and characterizing soil fungi for their physiological roles towards glyphosate degradation. Pure cultures of fungi were isolated from soil contaminated with glyphosate at farms in Lagos, Nigeria. The cultures were grown on minimal salt agar media amended with glyphosate. The best isolates exhibiting good tolerance to the glyphosate were characterized using molecular techniques. The BLAST search indicated that the fungi belong to four Aspergillus species (Aspergillus flavus strain JN-YG-3-5, Aspergillus niger strain APBSDSF96, Aspergillus fumigatus strain FJAT-31052 and Aspergillus flavus strain APBSWTPF130, Trichoderma gamsii and Penicillium simplicissimum. The biodegradation study of the glyphosate by the selected fungi species showed the presence of Aminomethylphosphonic Acid (AMPA) except for Aspergillus fumigatus strain FJAT-31052. Annotation analysis of the partial gene sequence shows that the strains possess protein coding gene clusters for glyphosate utilization and other physiological activities. The GhostKOALA output confirmed that CYP2W1 gene (Cytochrome P450, fungi type) was present in Aspergillus fumigatus strain FJAT-31052 which was absent in genome of other fungi. The physiological and molecular characteristics of Aspergillus fumigatus strain FJAT-31052 clearly show that this isolate is a useful organism for managing contamination by glyphosate pesticide.

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The molecular study of microbial and functional diversity of resistant microbes in heavy metal contaminated soil

Cited by 24 publications

References 25 publications

Heavy Metal-Resistant Filamentous Fungi as Potential Mercury Bioremediators

Heavy Metal-Resistant Filamentous Fungi as Potential Mercury Bioremediators

Physiological and molecular characterization of active fungi in pesticides contaminated soils for degradation of glyphosate

Physiological and molecular characterization of active fungi in pesticides contaminated soils for degradation of glyphosate

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