The molecular structures and expression patterns of zebrafish troponin I genes

Fu, Chuan-Yang; Tsai, Huai‐Jen

doi:10.1016/j.gep.2009.02.001

Cited by 23 publications

(21 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The direct evolutionary relationship between ssTnI and cTnI genes is supported by the facts that a) cTnI in lower vertebrates, such as zebra fish, aligned with the ssTnI monophyletic group (Fu et al, 2009) (Fig. 1), b) the unique N-terminal extension of mammalian and avian cTnI, which is absent in fast and slow skeletal muscle isoforms of TnI, is an adult heart specific structure added during evolution as it is absent in fish cTnI (Fig.…”

Section: Evolution Of Tni Isoform Genesmentioning

confidence: 95%

“…Interestingly, ssTnI is not expressed in the embryonic hearts of Xenopus and zebra fish, while it is expressed in the somites and skeletal muscles (Warkman and Atkinson, 2002; Fu et al, 2009). In the meantime, the expression of zebra fish cTnI is not restricted to cardiac muscle and is detectable in craniofacial muscles (Fu et al, 2009).…”

Section: Muscle Type-specific Expression and Developmental Regulatmentioning

confidence: 99%

“…In the meantime, the expression of zebra fish cTnI is not restricted to cardiac muscle and is detectable in craniofacial muscles (Fu et al, 2009). These observations indicate the high degree of similarity between lower vertebrate ssTnI and cTnI that lacks the N-terminal extension (Fig.…”

Section: Muscle Type-specific Expression and Developmental Regulatmentioning

confidence: 99%

See 2 more Smart Citations

TNNI1, TNNI2 and TNNI3: Evolution, regulation, and protein structure–function relationships

Sheng

Jin

2016

Gene

100

View full text Add to dashboard Cite

Troponin I (TnI) is the inhibitory subunit of the troponin complex in the sarcomeric thin filament of striated muscle and plays a central role in the calcium regulation of contraction and relaxation. Vertebrate TnI has evolved into three isoforms encoded by three homologous genes: TNNI1 for slow skeletal muscle TnI, TNNI2 for fast skeletal muscle TnI and TNNI3 for cardiac TnI, which are expressed under muscle type-specific and developmental regulations. To summarize the current knowledge on the TnI isoform genes and products, this review focuses on the evolution, gene regulation, posttranslational modifications, and structure-function relationship of TnI isoform proteins. Their physiological and medical significances are also discussed.

show abstract

Section: Evolution Of Tni Isoform Genesmentioning

confidence: 95%

Section: Muscle Type-specific Expression and Developmental Regulatmentioning

confidence: 99%

See 1 more Smart Citation

TNNI1, TNNI2 and TNNI3: Evolution, regulation, and protein structure–function relationships

Sheng

Jin

2016

Gene

100

View full text Add to dashboard Cite

show abstract

“…Moreover, the optical properties of the embryo make it highly amenable to light microscopy. Like in mouse and human, the troponin genes in zebrafish exist in several isoforms, differentially expressed in cardiac, slow-twitch or fast-twitch skeletal muscle (Fu et al, 2009;Hsiao et al, 2003). Additionally, troponin genes have been duplicated in the zebrafish genome relative to mammalian genomes.…”

Section: Introductionmentioning

confidence: 99%

Troponin T is essential for sarcomere assembly in zebrafish skeletal muscle

Ferrante

Kiff

Goulding

et al. 2011

Journal of Cell Science

View full text Add to dashboard Cite

SummaryIn striated muscle, the basic contractile unit is the sarcomere, which comprises myosin-rich thick filaments intercalated with thin filaments made of actin, tropomyosin and troponin. Troponin is required to regulate Ca 2+ -dependent contraction, and mutant forms of troponins are associated with muscle diseases. We have disrupted several genes simultaneously in zebrafish embryos and have followed the progression of muscle degeneration in the absence of troponin. Complete loss of troponin T activity leads to loss of sarcomere structure, in part owing to the destructive nature of deregulated actin-myosin activity. When troponin T and myosin activity are simultaneously disrupted, immature sarcomeres are rescued. However, tropomyosin fails to localise to sarcomeres, and intercalating thin filaments are missing from electron microscopic cross-sections, indicating that loss of troponin T affects thin filament composition. If troponin activity is only partially disrupted, myofibrils are formed but eventually disintegrate owing to deregulated actin-myosin activity. We conclude that the troponin complex has at least two distinct activities: regulation of actin-myosin activity and, independently, a role in the proper assembly of thin filaments. Our results also indicate that sarcomere assembly can occur in the absence of normal thin filaments.

show abstract

“…From these we con-473 structed orthologous groups using two di↵erent ortholog finders, InParanoid 474 and OrthoMCL [26,59], using default parameter settings. Both programs 475 were rated highly in benchmarking studies which analyzed the performance of 476 orthology-prediction methods [60,61]. For the purpose of detecting orthologs 477 of very diverged species, we chose OrthoMCL version 2.0.2 and the associ-478 ated MCL version 11-335, as they were shown to be more robust [60,61] than 479 similar programs.…”

mentioning

confidence: 99%

Concordant developmental expression profiles of orthologues in highly divergent Bilateria

Ferretti

Krämer-Eis

Schiffer

2019

Preprint

View full text Add to dashboard Cite

Bilateria are the predominant clade of animals on earth. Despite having evolved a large variety of body-plans and developmental modes, they are characterized by common morphological traits. However, it is not clear if clade-specific genes can be linked to these traits, distinguishing bilaterians from non-bilaterians, with their less complex body morphology. Comparing proteomes of bilaterian and non-bilaterian species in an elaborate computational pipeline we aimed to find and define a set of of bilaterian-specific genes. Finding no high-confidence set of such genes, we nevertheless detected an evolutionary signal possibly uniting the highly diverse bilaterian taxa. Using a novel multi-species GO-enrichment method, we determined the functional repertoire of genes that are widely conserved among Bilateria. We found that these genes contribute to morphogenesis, neuronal-system and muscle development, processes that have been described as di↵erent between bilaterians and non-bilaterians. Analyzing gene expression profiles in three very distantly related bilaterina species, we find characteristic peaks at comparable stages of development and a delayed onset of expression in embryos. In particular, the expression of the conserved genes appears to peak at the phylotypic stage of di↵erent bilaterian phyla. In summary, our data underpin ⇤ corresponding authors the orthologue conjecture and illustrate how development connects distantly related Bilateria after millions of years of divergence, pointing to processes potentially separating them from non-bilaterians. 93 filtering criteria. 94 2.2. Orthologues conserved across divergent Bilateria 95 In our analysis, we included the highly divergent bilaterian model or-96 ganisms Caenorhabditis elegans, Drosophila melanogaster, and Danio rerio.97 4These have very well curated and annotated genomes and are therefore help-98 ful to find genes which are also functionally conserved since more than 99 500Myrs of independent evolution. To take advantage of this, we decided 100 to use the intersection of sets M and L (termed L 0 = L \ M ) for further 101 analysis. Compared to set L (125 clusters), this intersection lacks 31 clusters 102 missing a C. elegans ortholog and 9 clusters missing an ortholog from D. 103 melanogaster, resulting in a set of 85 orthogroups. Except for A. californica, 104 the least represented species in these clusters is S. purpuratus (genes of this 105 species occur in 63 of 85 clusters). By construction the model organisms (C. 106 elegans, D. melanogaster, D. rerio) are represented in all clusters in L 0 . On 107 average, a (bilaterian) species is represented in 93.1% of the clusters.108 2.3. Clade-specificity declines with data availability 109 A general drawback of our experimental procedure is its reliance on cor-110 rectly identified and annotated genes. Since the majority of the organisms 111 in our study are non-model organisms, they may su↵er from incomplete or 112 erroneous gene annotation. For example, we omitted the bilaterian species 113 A. californic...

show abstract

The molecular structures and expression patterns of zebrafish troponin I genes

Cited by 23 publications

References 39 publications

TNNI1, TNNI2 and TNNI3: Evolution, regulation, and protein structure–function relationships

TNNI1, TNNI2 and TNNI3: Evolution, regulation, and protein structure–function relationships

Troponin T is essential for sarcomere assembly in zebrafish skeletal muscle

Concordant developmental expression profiles of orthologues in highly divergent Bilateria

Contact Info

Product

Resources

About