The molecular phylogeny and taxonomy of endophytic fungal species from the leaves of Vitex negundo L.

Abstract

Enormous fungal species live within the healthy plant tissues, some of which presumably occur in a symbiotic association with host. Some fungal endophytes are widespread and can be found in many different plant species, whereas others are highly specific to single hosts. In this study, we isolated three endophytic fungi from the medicinal plant Vitex negundo. They were identified based on morphological characteristics such as size, shape, and colour of the spore and it was reinforced by 18s rRNA gene sequence analysis. The phylogenetic tree showed that C. gloeosporioides VN1 and Pestalotiopsis virgatula VN2 were closely relationship between. But they were not closely relationship between the other endophytic fungal species that were obtained from geographically different part of the world. This aspect can be further explored to understand the relationships between plant hosts and their fungal endophyte.

Fungal endophytes of Himalayan Cold Desert Induces Heat tolerance in Rice (Oryza sativa L.)

“…These endophytes were identi ed using ITS region of rDNA as P. funiculosum, C. lacerate and E. endophytica. The ITS region of rDNA sequences is widely used to examine phylogenetic positions or relationship of a species because this region are anked by preserved segments (18S, 5.8S and 28S genes) which provide information about the phylogeny and the taxonomic level, since their evolution is slow and they are highly similar within different taxa (Ramesh et al, 2017).…”

Fungal endophytes of Himalayan Cold Desert Induces Heat tolerance in Rice (Oryza sativa L.)

“…lacerate and E. endophytica. The ITS region of rDNA sequences is widely used to examine phylogenetic positions or relationship of a species because this region are flanked by preserved segments (18S, 5.8S and 28S genes) which provide information about the phylogeny and the taxonomic level, since their evolution is slow and they are highly similar within different taxa (Ramesh et al, 2017).…”

Antimicrobial Activities of Endophytic Fungal Crude Extracts Isolated from Cashew Tree (Anacardium occidentale)

“…PCR amplification of the fungal rDNA internal transcribed spacers (ITS) regions of all the isolates were done using the primer pairs ITS1 and ITS4. PCR amplifications conditions included 1 cycle of 94 °C for 3 min, followed by 35 cycles of 94 °C for 30 sec, 55 °C for 1 min, and 72 °C for 1 min and a final extension cycle of 72 °C for 10 min (Ramesh et al 2017). The PCR products were checked for quality using gel electrophoresis.…”