The molecular diversity analysis of Auricularia auricula-judae in China by nuclear ribosomal DNA intergenic spacer

Li, Li; Zhong, Caihong; Bian, Yinbing

doi:10.1016/j.ejbt.2013.12.005

Cited by 14 publications

(10 citation statements)

References 25 publications

(28 reference statements)

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“…Edible mushrooms have been exploited for their nutritional and medicinal properties for centuries, and have been used as a significant therapeutic raw material in folk medicines [1]. Auricularia heimuer [2] is an edible and medicinal mushroom that has been widely cultivated for thousands of years in China [3] and is acknowledged as a healthy food. The fruiting bodies contain a high content of carbohydrates, proteins, minerals, and many other nutrient components [4].…”

Section: Introductionmentioning

confidence: 99%

Cloning and expression analyses of a cellobiohydrolase gene from Auricularia heimuer

Sun

Wang

et al. 2019

Biotechnology & Biotechnological Equipment

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A cellobiohydrolase gene was cloned and analyzed from Auricularia heimuer, and named A-cbh. The cDNA sequence was submitted to GenBank under the accession number MK620906. Sequence analysis showed that the cDNA comprised 1551 bp and encoded a putative polypeptide of 516 amino acids. The molecular weight of this protein was predicted as 54.44 kDa, and the theoretical isoelectric point was 5.24. The recombinant prokaryotic expression vector pET32a-A-cbh was constructed and transformed into Escherichia coli BL21. Sodium dodecyl sulphate polyacrylamide gel electrophoresis indicated that the recombinant protein had molecular size between 66.2 kDa and 94.0 kDa, which was consistent with our expectations because a tag protein of approximately 20 kDa was present in the vector of pET32a. The transcription levels of A-cbh in the mycelium were lower than those in the fruiting bodies. The highest level of A-cbh expression was observed in the primordium, which was 8.65-fold higher than that in the control (mycelia at 6 days). We speculated that A-cbh might be involved in the formation of primordia and the development of fruiting bodies. The results of the present study will form the basis for further research into the function of the cellobiohydrolase in A. heimuer.

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Section: Introductionmentioning

confidence: 99%

Cloning and expression analyses of a cellobiohydrolase gene from Auricularia heimuer

Sun

Wang

et al. 2019

Biotechnology & Biotechnological Equipment

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“…Mushrooms of the genus Auricularia, commonly known as wood ear mushrooms, are edible and medici-Auricularia are currently the fourth most important cultivated mushrooms, after Agaricus spp., Pleurotus spp., and Lentinus edodes. 1,2 China is the largest producer; production in Malaysia is low because of a lack of technology.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Properties and Yield of Wood Ear Mushroom, Auricularia polytricha (Agaricomycetes), Cultivated on Rubberwood Sawdust

Teoh

Ahmad

Johari

et al. 2018

Int J Med Mushrooms

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Mushroom cultivation has become an important component of agriculture, providing food and contributing to the global economy. It uses vertical space and addresses issues of food quality, health improvement, and environmental sustainability. Auricularia mushrooms are popular ingredients in traditional Chinese cuisine. The objective of this study was to determine yield and evaluate radical scavenging capacity of A. polytricha cultivated on rubberwood sawdust on a large scale; we measured total phenolic content; DPPH, hydroxyl, superoxide anion, and peroxyl radical scavenging; and reducing power. Cultivation on rubberwood sawdust produces an average of 4 harvests per bag and a biological efficiency of 80-82%. The antioxidant capacity investigations revealed that the ethyl acetate fraction was the most potent radical scavenger in all assays except that for superoxide anions, whereas the aqueous fraction exhibited mild to moderate antioxidant capacity in scavenging the various radicals. Artificial cultivation of A. polytricha on rubberwood sawdust yields many sporophores with potent antioxidant capacity.

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“…Heterogeneity in the length of the IGS has been reported in fungi [ 10 , 11 ]. In addition, the possibility of using IGS regions in discriminatory analysis among intra-specific individuals of mushroom species such as Auricularia auricula-judae , Laccaria bicolor , and Lentinula edodes has been suggested [ 12 , 13 , 14 ]. Thus, it is thought that IGS region I information is also useful for the characterization of the heterogeneity of monokaryotic strains of A. bisporus .…”

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confidence: 99%

Genetic and Biochemical Characterization of Monokaryotic Progeny Strains of Button Mushroom (Agaricus bisporus)

et al. 2015

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To promote the selection of promising monokaryotic strains of button mushroom (Agaricus bisporus) during breeding, 61 progeny strains derived from basidiospores of two different lines of dikaryotic parental strains, ASI1038 and ASI1346, were analyzed by nucleotide sequencing of the intergenic spacer I (IGS I) region in their rDNA and by extracellular enzyme assays. Nineteen different sizes of IGS I, which ranged from 1,301 to 1,348 bp, were present among twenty ASI1346-derived progeny strains, while 15 different sizes of IGS I, which ranged from 700 to 1,347 bp, were present among twenty ASI1038-derived progeny strains. Phylogenetic analysis of the IGS sequences revealed that different clades were present in both the ASI10388- and ASI1346-derived progeny strains. Plating assays of seven kinds of extracellular enzymes (β-glucosidase, avicelase, CM-cellulase, amylase, pectinase, xylanase, and protease) also revealed apparent variation in the ability to produce extracellular enzymes among the 40 tested progeny strains from both parental A. bisporus strains. Overall, this study demonstrates that characterization of IGS I regions and extracellular enzymes is useful for the assessment of the substrate-degrading ability and heterogenicity of A. bisporus monokaryotic strains.

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The molecular diversity analysis of Auricularia auricula-judae in China by nuclear ribosomal DNA intergenic spacer

Cited by 14 publications

References 25 publications

Cloning and expression analyses of a cellobiohydrolase gene from Auricularia heimuer

Cloning and expression analyses of a cellobiohydrolase gene from Auricularia heimuer

Antioxidant Properties and Yield of Wood Ear Mushroom, Auricularia polytricha (Agaricomycetes), Cultivated on Rubberwood Sawdust

Genetic and Biochemical Characterization of Monokaryotic Progeny Strains of Button Mushroom (Agaricus bisporus)

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