Cloning and expression analyses of a cellobiohydrolase gene from <i>Auricularia heimuer</i>

Sun, Jian; Wang, Shixin; Wang, Xutong; Sun, Tingting; Zou, Li

doi:10.1080/13102818.2019.1665477

Cited by 4 publications

(4 citation statements)

References 26 publications

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“…SDS-PAGE analysis of the recombinant Aa-bgl protein revealed bands with a molecular weight of $113.969 kDa between the 100 kDa and 135 kDa markers (Figure 7b), consistent with the theoretical molecular weight of the recombinant fusion protein [26]. Additionally, SDS-PAGE showed that protein expression was highest when induced for 10 h.…”

Section: Sds-page Analysis Of the Aa-bgl Proteinsupporting

confidence: 76%

Molecular cloning and bioinformatics analyses of a GH3 beta-glucosidase from Auricularia heimuer

Sun

et al. 2020

Biotechnology & Biotechnological Equipment

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The present study reports the isolation and analysis of a beta-glucosidase gene (accession number MN816859) from the fungus Auricularia heimuer. The Aa-bgl gene comprises 2583 bp and encodes a putative polypeptide of 860 amino acids. BlastP analysis revealed conserved glycoside hydrolase family 3 domains. Recombinant plasmid pET-32a-bgl was constructed to overexpress the target protein in Escherichia coli BL21, and sodium dodecyl sulphate polyacrylamide gel electrophoresis analysis revealed target bands between 100 kDa and 135 kDa, consistent with the expected molecular weight. Additionally, the transcription levels of Aa-bgl at different developmental stages were investigated, and the results showed that the expression levels increased with the maturation of the fruiting bodies. In mature fruiting bodies, the levels were 6.69-fold higher than in controls (mycelium at 6 days). We therefore speculate that Aa-bgl may be correlated with development in A. heimuer. The findings provide a theoretical basis for studying the function of this beta-glucosidase in future work.

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Section: Sds-page Analysis Of the Aa-bgl Proteinsupporting

confidence: 76%

Molecular cloning and bioinformatics analyses of a GH3 beta-glucosidase from Auricularia heimuer

Sun

et al. 2020

Biotechnology & Biotechnological Equipment

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“…The protein motif was conducted by using the Simple Modular Architecture Research Tool (SMART) ( http://smart.embl-heidelberg.de/ ). The phylogenetic tree was constructed using Clustalx2.1 and MEGA7.0 [ 18 ].…”

Section: Methodsmentioning

confidence: 99%

Cloning and expression analyses of a Pyrabactin Resistance 1 (PYR1) gene from Magnolia sieboldii K. Koch

et al. 2021

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Magnolia sieboldii K. Koch is endemic to China and has high medicinal and ornamental values. However, its seed exhibits morphophysiological dormancy, and the molecular mechanisms of which are not clearly understood. To reveal the regulation mechanism of the ABA signal in seed dormancy, the M. sieboldii ABA receptor Pyrabactin Resistance 1 (PYR1) gene was cloned and analyzed. Analysis of the MsPYR1 sequence analysis showed that the full-length cDNA contained a complete open reading frame of 987 bp and encoded a predicted protein of 204 amino acid residues. The protein had a relative molecular weight of 22.661 kDa and theoretical isoelectric point of 5.01. The transcript levels of MsPYR1 were immediately upregulated at 16 DAI and then decreased at 40 DAI. The highest transcript level of MsPYR1 was found in the dry seeds, indicating that the MsPYR1 gene may play an important role in the regulation of dormancy. The MsPYR1 gene cDNA was successfully expressed in E. coli Rosetta (DE3), and the protein bands were consistent with the prediction. The Anti-MsPYR1antibody could detect the expression of MsPYR1 in M. sieboldii. The results provided a foundation for further study of the function of the MsPYR1 gene.

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“…Similar SbIDI protein sequences from different species were downloaded via the BLAST algorithm at GenBank. Using MEGA 6.0 software for IDI sequence alignment, a phylogenetic tree was constructed using the Neighbor-Joining method, with the repeated sampling frequency set as 1000 replicates to ensure the stability of the branches of the tree [13].…”

Section: Bioinformatics Analysis Of the Sbidi Genementioning

confidence: 99%

Cloning, molecular properties and differential expression analysis of the isopentenyl diphosphate isomerase gene in Sanghuangporus baumii

Liu

Sun

Wang

2020

Biotechnology & Biotechnological Equipment

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Sanghuangporus baumii (Pil at) L.W. Zhou & Y.C. Dai, a popular medicinal fungus, has been used to treat several diseases by its triterpenoids bioactive ingredient for many years. However, the triterpenoids content of S. baumii is low and their biosynthesis mechanisms are not clearly understood. In order to reveal the regulation mechanism of triterpenoids biosynthesis in S. baumii, the cDNA encoding isopentenyl diphosphate isomerase (IDI) involved in triterpenoids biosynthesis was cloned and named as SbIDI (GenBank number MK955885). The open reading frame of the SbIDI gene cDNA comprised 792 bp and encoded a polypeptide of 263 amino acids with a predicted protein molecular weight of 29.80 kDa. The transcript level of the SbIDI gene and triterpenoids content of S. baumii were detected at different developmental stages. The results showed that the transcript level of the SbIDI gene and triterpenoids content had almost the same trend of change, increased first and then decreased dynamically. The highest transcript level of the SbIDI gene occurred earlier than the highest triterpenoids content, indicating that the SbIDI gene may play an important role in triterpenoids biosynthesis in S. baumii. Moreover, the SbIDI gene cDNA was successfully expressed in Escherichia coli BL21 (DE3), and the protein bands were consistent with the prediction.The results will lay a foundation for further study of the function of the SbIDI gene.

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Cloning and expression analyses of a cellobiohydrolase gene from Auricularia heimuer

Cited by 4 publications

References 26 publications

Molecular cloning and bioinformatics analyses of a GH3 beta-glucosidase from Auricularia heimuer

Molecular cloning and bioinformatics analyses of a GH3 beta-glucosidase from Auricularia heimuer

Cloning and expression analyses of a Pyrabactin Resistance 1 (PYR1) gene from Magnolia sieboldii K. Koch

Cloning, molecular properties and differential expression analysis of the isopentenyl diphosphate isomerase gene in Sanghuangporus baumii

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