The molecular correlates of organ loss: the case of insect Malpighian tubules

Jing, Xin; White, Thomas A.; Yang, Xiaowei; Douglas, Angela E.

doi:10.1098/rsbl.2015.0154

Cited by 11 publications

(13 citation statements)

References 18 publications

(19 reference statements)

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“…Neuropeptides of the CAPA family have roles in the stimulation of fluid secretion in Malpighian (renal) tubules and, more recently, have been linked to desiccation and cold tolerance in Drosophila . Unlike most insects, aphids lack Malpighian tubules; organs with vital roles in osmoregulation, detoxification and immunity . Due to this secondary loss of Malpighian tubules in the aphids, key osmoregulatory roles have been reassigned to other organs, particularly the aphid gut .…”

Section: Discussionmentioning

confidence: 99%

“…17 Unlike most insects, aphids lack Malpighian tubules; 48 organs with vital roles in osmoregulation, detoxification and immunity. 49,50 Due to this secondary loss of Malpighian tubules in the aphids, key osmoregulatory roles have been reassigned to other organs, particularly the aphid gut. 50 Receptor mapping assays performed in the current study offer support to this, highlighting the presence of CAPA receptors along the aphid gut and implicating the gut as a primary target for CAPA neuropeptide action.…”

Section: Discussionmentioning

confidence: 99%

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Assessment of neuropeptide binding sites and the impact of biostable kinin and CAP2b analogue treatment on aphid (Myzus persicae and Macrosiphum rosae) stress tolerance

Alford

Marley

Dornan

et al. 2019

Pest Management Science

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BACKGROUND Neuropeptides are regulators of critical life processes in insects and, due to their high specificity, represent potential targets in the development of greener insecticidal agents. Fundamental to this drive is understanding neuroendocrine pathways that control key physiological processes in pest insects and the screening of potential analogues. The current study investigated neuropeptide binding sites of kinin and CAPA (CAPA‐1) in the aphids Myzus persicae and Macrosiphum rosae and the effect of biostable analogues on aphid fitness under conditions of desiccation, starvation and thermal (cold) stress. RESULTS M. persicae and M. rosae displayed identical patterns of neuropeptide receptor mapping along the gut, with the gut musculature representing the main target for kinin and CAPA‐1 action. While kinin receptor binding was observed in the brain and VNC of M. persicae , this was not observed in M. rosae . Furthermore, no CAPA‐1 receptor binding was observed in the brain and VNC of either species. CAP2b/PK analogues (with CAPA receptor cross‐activity) were most effective in reducing aphid fitness under conditions of desiccation and starvation stress, particularly analogues 1895 ( 2Abf ‐ Suc ‐FGPRLa) and 2129 ( 2Abf ‐ Suc ‐ A TPRIa), which expedited aphid mortality. All analogues, with the exception of 2139‐Ac, were efficient at reducing aphid survival under cold stress, although were equivalent in the strength of their effect. CONCLUSION In demonstrating the effects of analogues belonging to the CAP2b neuropeptide family and key analogue structures that reduce aphid fitness under stress conditions, this research will feed into the development of second generation analogues and ultimately the development of neuropeptidomimetic‐based insecticidal agents. © 2019 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Assessment of neuropeptide binding sites and the impact of biostable kinin and CAP2b analogue treatment on aphid (Myzus persicae and Macrosiphum rosae) stress tolerance

Alford

Marley

Dornan

et al. 2019

Pest Management Science

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“…Oral secretions and salivary components provide important early defenses and aid in host plant adaptation . In contrast, A. nerii lack Malpighian tubules, and thus, their gut tissues may play important roles in detoxification (Jing et al, 2015). Aphid saliva contains proteins that inactivate plant phytochemicals and prevent plant defenses that deter aphid feeding (Miles, 1999;Will et al, 2007;Elzinga et al, 2014).…”

Section: Aphids and Milkweedsvalues Of The Systemmentioning

confidence: 99%

“…Monarchs have evolved amino acid substitutions in their Na + /K + ATPases at the binding site of cardenolides which provide protection from toxicity (Dobler et al, 2012). A recent study comparing gene expression between pea aphid [Acyrthosiphon pisum (Harris)] gut tissues and D. melanogaster Malpighian tubules found that over 50% of the genes expressed in D. melanogaster Malpighian tubules had expressed homologs in the aphid guts, especially those involved in metabolic processes (Jing et al, 2015). (4) The insect gut as a barrier.…”

Section: Aphids and Milkweedsvalues Of The Systemmentioning

confidence: 99%

Insect adaptations toward plant toxins in milkweed–herbivores systems – a review

Birnbaum

Abbot

2018

Entomologia Exp Applicata

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Studies of plant defenses and insect herbivores have been important in the development of our understanding of coevolution and specialization. Milkweed-herbivore systems have been a model for studying plant secondary chemistry defense evolution, insect adaptations to that chemistry, and coevolution between toxic plants and their herbivores for over a century. Yet, we are only beginning to unravel the multitude of adaptations required for insect specialization on milkweed plants. We review the empirical evidence for specialist insect adaptations toward milkweed toxins, coevolution between insects and milkweed plants, and canonical paradigms for sequestration and highlight the areas for further research. By comparing research performed with diverse milkweed insects, we discuss the need to comprehensively study adaptations and specialization in divergent insect taxa.

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“… *RNAseq transcriptome data sets of Acyrthosiphon pisum whole body and guts (NCBI Short Read Archive: accession no. srp053295; Jing et al , () were also used).…”

Section: Introductionmentioning

confidence: 99%

Evolutionary conservation of candidate osmoregulation genes in plant phloem sap‐feeding insects

Jing

White

Luan

et al. 2016

Insect Molecular Biology

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The high osmotic pressure generated by sugars in plant phloem sap is reduced in phloem-feeding aphids by sugar transformations and facilitated water flux in the gut. The genes mediating these osmoregulatory functions have been identified and validated empirically in the pea aphid Acyrthosiphon pisum: sucrase 1 (SUC1), a sucrase in glycoside hydrolase family 13 (GH13), and aquaporin 1 (AQP1), a member of the Drosophila integral protein (DRIP) family of aquaporins. Here, we describe molecular analysis of GH13 and AQP genes in phloem-feeding representatives of the four phloem-feeding groups: aphids (Myzus persicae), coccids (Planococcus citri), psyllids (Diaphorina citri, Bactericera cockerelli) and whiteflies (Bemisia tabaci MEAM1 and MED). A single candidate GH13-SUC gene and DRIP-AQP gene were identified in the genome/transcriptome of most insects tested by the criteria of sequence motif and gene expression in the gut. Exceptionally, the psyllid Ba. cockerelli transcriptome included a gutexpressed Pyrocoelia rufa integral protein (PRIP)-AQP, but has no DRIP-AQP transcripts, suggesting that PRIP-AQP is recruited for osmoregulatory function in this insect. This study indicates that phylogenetically related SUC and AQP genes may generally mediate osmoregulatory functions in these diverse phloem-feeding insects, and provides candidate genes for empirical validation and development as targets for osmotic disruption of pest species.

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The molecular correlates of organ loss: the case of insect Malpighian tubules

Cited by 11 publications

References 18 publications

Assessment of neuropeptide binding sites and the impact of biostable kinin and CAP2b analogue treatment on aphid (Myzus persicae and Macrosiphum rosae) stress tolerance

Assessment of neuropeptide binding sites and the impact of biostable kinin and CAP2b analogue treatment on aphid (Myzus persicae and Macrosiphum rosae) stress tolerance

Insect adaptations toward plant toxins in milkweed–herbivores systems – a review

Evolutionary conservation of candidate osmoregulation genes in plant phloem sap‐feeding insects

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