“…Among these milkweed herbivores, sequestration of cardenolides is often linked to the gain of target‐site insensitivity in the otherwise highly conserved Na + /K + ‐ATPase enzyme (Dobler et al., ; Zhen, Aardema, Medina, Schumer, & Andolfatto, ); in fact, the defensive benefit from cardenolide sequestration may have been a key evolutionary driver of target‐site insensitivity (Petschenka & Agrawal, ). As a prominent exception among cardenolide‐sequestering herbivores, A. nerii lacks a resistant Na + /K + ‐ATPase (Zhen et al., ), and instead may primarily rely on cardenolide detoxification (Birnbaum & Abbot, ; Birnbaum, Rinker, Gerardo, & Abbot, ). Furthermore, while sequestration of cardenolides in most herbivores involves at least partially active uptake (Dobler et al., ), sequestration by A. nerii appears to be primarily passive (Züst & Agrawal, 2016b).…”