“…When adsorbed fibronectin, the stability of the adsorbed protein-material interface increased with the increasing of stiffness of the RSF substrates. Finally, larger areas of cytoskeleton-associated focal adhesions, higher orders of cytoskeletal organization and more elongated cell spreading were all observed for BMSCs cultured on RSF substrates with high stiffness than lower ones, along with enhanced nuclear translocation and activation of YAP/TAZ [ 133 ]. The stiff RSF substrate with higher β -sheet content further enhanced the osteogenesis of BMSCs, evidenced by higher expression of ALP and collagen Ι, and also denser deposition of calcium [ 133 ], as presented in Fig.…”