The molecular characterisation of the polysaccharide gum from Acacia senegal

Osman, Mohamed E.; Menzies, Alan R.; Williams, Peter A.; Phillips, Glyn O.; Baldwin, Timothy C.

doi:10.1016/0008-6215(93)84042-5

Cited by 109 publications

(79 citation statements)

References 26 publications

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“…112, 1996 from the polypeptide backbone of PM-AGP2 (Fincher et al, 1983;Qi et al, 1991). The extent to which this difference causes dissimilarities in the shapes of these molecules might be investigated by biophysical methods such as transmission electron microscopy (Qi et al, 1991;Baldwin et al, 1993) or by Fourier transform IR polarized microspectrometry (Baldwin et al, 1993).…”

Section: After Incubation Of Plasma Membrane Vesicles In 1%mentioning

confidence: 99%

“…Bacic et al, 1987;Komalavilas et al, 1991) and AGPs copiously exuded as tears from trees, such as gum arabic from Acacia senegal (Akiyama et al, 1984;Qi et al, 1991;Osman et al, 1993). These AGPs have molecular masses of 60 to 300 kD and consist of 90 to 95% carbohydrate.…”

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confidence: 99%

“…suspension-cultured cells (Bacic et al, 1987;Gleeson et al, 1989;Komalavilas et al, 1991;Chen et al, 1994), in stylar secretions (Gleeson and Clark, 1979;Gell et al, 1986;Du et al, 1994;Cheung et al, 1995), and in plant gums and mucilages (Akiyama et al, 1984;Osman et al, 1993).…”

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confidence: 99%

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Heterogeneity of Arabinogalactan-Proteins on the Plasma Membrane of Rose Cells

Serpe

Nothnagel

1996

Plant Physiology

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Arabinogalactan-proteins (AGPs) have been purified from the plasma membrane of suspension-cultured Paul's Scarlet rose (Rosa sp.) cells. The two most abundant and homogeneous plasma membrane ACP fractions were named plasma membrane ACPl (PM-ACP1) and plasma membrane AGP2 (PM-ACP2) and had apparent molecular masses of 140 and 21 7 kD, respectively. Both PM-ACP1 and PM-ACP2 had p-(l-3)-, p-(1,6)-, and p-(1,3,6)-galactopyranosyl residues, predominantly terminal a-arabinofuranosyl residues, and (1,4)-and terminal glucuronopyranosyl residues. The protein moieties of PM-ACP1 and PM-ACP2 were both rich in hydroxyprolhe, alanine, and serine, but differed in the abundance of hydroxyproline, which was 1.6 times higher in PM-ACP2 than in PM-ACP1. Another difference was the overall protein content, which was 3.7% (w/w) in PM-ACP1 and 15% in PM-ACP2. As judged by their behavior on reverse-phase chromatography, PMACPl and PM-ACP2 were not more hydrophobic than ACPs from the cell wall or culture medium. In contrast, a minor plasma membrane ACP fraction eluted later on reverse-phase chromatography and was more negatively charged at pH 5 than either PM-ACP1 or PM-ACP2. The more negatively charged fraction contained molecules with a glycosyl composition characteristic of ACPs and included at least two different macromolecules. The results of this investigation indicate that Rosa plasma membrane contains at least four distinct ACPs or ACP-like molecules. These molecules differed from each other in size, charge, hydrophobicity, amino-acyl composition, and/or protein content.AGPs are a class of proteoglycans that are rich in galactosyl and arabinosyl residues Fincher et al., 1983; Bacic et al., 1988), and they appear to be present in all plant cells (Jermyn and Yeow, 1975;

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Section: After Incubation Of Plasma Membrane Vesicles In 1%mentioning

confidence: 99%

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confidence: 99%

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Heterogeneity of Arabinogalactan-Proteins on the Plasma Membrane of Rose Cells

Serpe

Nothnagel

1996

Plant Physiology

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“…The side chains are composed of two to five 1→3-linked β-D-galactopyranosyl units, joined to the main chain by 1→6-linkages. Both the main and the side chains contain units of α-L-arabinofuranosyl, α-L-rhamnopyranosyl, β-D glucuronopyranosyl, and 4-O-methyl-β-D-glucuronopyranosyl units, the latter two mostly as end-units [81][82][83]. It is suggested that the high-molecular-weight fraction of the gum consists of large carbohydrate blocks with a molecular mass of approximately 2.5 · 10 5 Da attached individually to a polypeptide chain.…”

Section: Tree Gums -An Overviewmentioning

confidence: 99%

Green Synthesis: Nanoparticles and Nanofibres Based on Tree Gums for Environmental Applications

Padil

Černík

2016

Ecological Chemistry and Engineering S

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Abstract:The recent advances and potential applications of nanoparticles and nanofibres for energy, water, food, biotechnology, the environment, and medicine have immensely conversed. The present review describes a 'green' method for the synthesis and stabilization of nanoparticles and 'green electrospinning' both using tree gums (arabic, tragacanth, karaya and kondagogu). Furthermore, this review focuses on the impending applications of both gum stabilized nanoparticles and functionalized membranes in remediation of toxic metals, radioactive effluents, and the adsorptive removal of nanoparticulates from aqueous environments as well as from industrial effluents. Besides, the antibacterial properties of gum derivatives, gum stabilized nanoparticles, and functionalized electrospun nanofibrous membranes will also be highlighted. The functionalities of nanofibrous membranes that can be enhanced by various plasma treatments (oxygen and methane, respectively) will also be emphasized.

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“…Considerable progress has been made in recent years in gaining a more definitive description of the structure of the gum through characterization of fractions isolated by various separation procedures, including gel permeation chromatography (GPC) (Vandevelde and Fenyo 1985, Randall et al 1988, Qi et al 1991, Idris et al 1998, hydrophobic interaction chromatography (HIC), (Randall et al 1989, Osman et al 1993 and ion exchange chromatography (Osman et al 1995). This paper describes further work we have undertaken with the use of HIC to fractionate two gum samples obtained from different regions in Sudan.…”

Section: Introductionmentioning

confidence: 97%