The molecular biology of plant acclimation to low temperature

Hughes, Monica A.; Dunn, Michael

doi:10.1093/jxb/47.3.291

Cited by 375 publications

(228 citation statements)

References 93 publications

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“…Although the expression of some cold-inducible genes seems to be specifically regulated by low temperature, in most cases it is also regulated by water stress and ABA (Hughes and Dunn, 1996). This is in agreement with the observation that both drought and exogenous ABA treatments can mimic the effects of low temperatures and increase the freezing tolerance of several temperate species (Cloutier and Siminovitch, 1982;Chen and Gusta, 1983;Mantyla et al, 1995).…”

supporting

confidence: 88%

“…This adaptive process, known as cold acclimation (Levitt, 1980;Sakai and Larcher, 1987), involves a number of biochemical and physiological changes, including increased levels of soluble sugars, proteins, amino acids, and organic acids, as well as the modification of membrane lipid composition (Thomashow, 1994). Many of these changes have been shown to be regulated through changes in gene expression, and a number of genes, the expression of which is induced by low temperatures, have been isolated and characterized from a wide range of both dicot and monocot species (for review, see Thomashow, 1994, andDunn, 1996). The precise role that such changes in gene expression play in de Ia CoruAa, Km.…”

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Two Homologous Low-Temperature-Inducible Genes from Arabidopsis Encode Highly Hydrophobic Proteins

et al. 1997

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We have characterized two related cDNAs (RCIM and RC12B) corresponding to genes from Arabidopsis thaliana, the expression of which is transiently induced by low, nonfreezing temperatures.RClZA and R C D B encode small(54 amino acids), highly hydrophobic proteins that bear two potential transmembrane domains. They show similarity to proteins encoded by genes from barley (Hordeum vulgare L.) and wheatgrass (Lophophyrum elongatum) that are regulated by different stress conditions. Their high leve1 of sequence homology (78%) and their genomic location in a single restriction fragment suggest that both genes originated as a result of a tandem duplication. However, their regulatory sequences have diverged enough to confer on them different expression patterns. Like most of the cold-inducible plant genes characterized, the expression of RC12A and RC/ZB is also promoted by abscisic acid (ABA) and dehydration but is nota general response to stress conditions, since it is not induced by salt stress or by anaerobiosis. Furthermore, low temperatures are able to induce RC12A and RC12B expression in ABA-deficient and -insensitive genetic backgrounds, indicating that both ABA-dependent and 4ndependent pathways regulate the lowtemperature responsiveness of these two genes.

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confidence: 99%

Two Homologous Low-Temperature-Inducible Genes from Arabidopsis Encode Highly Hydrophobic Proteins

et al. 1997

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“…A gene wcs120 and its related, perhaps paralogous genes including Wcor39, Wcs66, Wcor80, Wcor200 and Wcor726 are expressed in response to low temperature (Houde et al, 1992;Guo et al, 1992;Quellet et al, 1993;Chauvin et al, 1994;Danyluk et al, 1996;Hughes and Dunn, 1996). Among them, wcs120 and wcor200 are the most well characterized members respectively encoding glycine-rich neutral proteins of 50 kDa (pI = 7.3) and 200 kDa (pI = 6.5) with characteristics of the group 2 LEA (or D-11) family, although they do not have a conserved polyserine sequence (Houde et al, 1992;Quellet et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

A cold-responsive wheat (Triticum aestivum L.) gene wcor14 identified in a winter-hardy cultivar 'Mironovska 808'.

et al. 2000

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“…The diploid cereal barley (Hordeum vulgare L.) genome contains at least 8 LTP genes distributed on 3 chromosomes (Gausing, 1994;Hughes and Dunn, 1996;Kalla et al, 1994). A LTP gene family (blt4), located on chromosome 3, was isolated because members are upregulated in young winter barley plants by a low positive temperature (6-2°C) which will acclimate the cultivar for frost tolerance (Dunn et al, 1991;Dunn et al, 1994;White et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Homology modelling and molecular dynamics aided analysis of ligand complexes demonstrates functional properties of lipid‐transfer proteins encoded by the barley low‐temperature‐inducible gene family, blt4

Keresztessy

Hughes

1998

The Plant Journal

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SummaryThe homology modelling technique was used to predict the tertiary structures of three members of the lowtemperature-inducible barley vegetative shoot epidermal lipid-transfer protein (LTP) family, BLT4, on the basis of the X-ray crystallographically determined three-dimensional structure of a maize seedling LTP. Differences between the maize LTP and the BLT4 family include amino acid substitutions around the entrance and inside the predicted hydrophobic binding tunnels of these proteins. Because of the deletion of the loop region corresponding to Val60-Gly62 of the maize LTP from all three BLT4 LTPs, their internal hydrophobic tunnels are longer. Molecular dynamics modelling shows that BLT4.9 can accommodate hexadecanoic acid in its binding tunnel in similar conformation to the maize LTP. However, modelled cis,cis-9,12-octadecandienoic acid had a more favourable interaction with the BLT4.9 LTP than with the maize protein. Di-cis,cis-9,12-octadecandienoyl phosphatidylglycerol and di-cis,cis-9,12-octadecandienoyl phosphatidylcholine were modelled in the BLT4.9 structure with the fatty acyl group at position 1 embedded in the binding tunnel and the group at position 2 located on the solvent accessible surface of the protein. The results of the modelling suggest that the phospholipid headgroup can form hydrogen and salt bridges with polar and charged residues outside the binding tunnel and the exposed hydrocarbon chain interacts with hydrophobic amino acids on the surface. These results are consistent with the proposal that BLT4 LTPs have a lipid-transfer function associated with frost acclimation in barley.

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The molecular biology of plant acclimation to low temperature

Cited by 375 publications

References 93 publications

Two Homologous Low-Temperature-Inducible Genes from Arabidopsis Encode Highly Hydrophobic Proteins

Two Homologous Low-Temperature-Inducible Genes from Arabidopsis Encode Highly Hydrophobic Proteins

A cold-responsive wheat (Triticum aestivum L.) gene wcor14 identified in a winter-hardy cultivar 'Mironovska 808'.

Homology modelling and molecular dynamics aided analysis of ligand complexes demonstrates functional properties of lipid‐transfer proteins encoded by the barley low‐temperature‐inducible gene family, blt4

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