A cold-responsive wheat (Triticum aestivum L.) gene wcor14 identified in a winter-hardy cultivar 'Mironovska 808'.

Tsvetanov, Sergei; Ohno, Ryoko; Tsuda, Kazuki; Takumi, Shigeo; Mori, Naoki; Atanassov, A.; Nakamura, Chiharu

doi:10.1266/ggs.75.49

Cited by 48 publications

(42 citation statements)

References 30 publications

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“…Using this eDNA library and the barley probes, we isolated several new Cor members · of wheat including Wcor14, Wcor14-wdhn13, Wrab19 and Wrab17, and WltJO (72). Wcor14 encodes a 14 kDa acidic (pi=4.71) and hydrophobic protein which is specifically induced by low temperature (73). Based on the study of structure and mode of expression, we concluded that W cor 14 is a_}yhea~ orthologue of the barley Cor14b.…”

Section: Cold Responsive Gene/protein Families and Cold/freezing mentioning

confidence: 91%

Gold Responsive Gene/Protein Families and Cold/Freezing Tolerance in Cereals

Tsvetanov

Atanassov²,

Nakamura

2000

Biotechnology & Biotechnological Equipment

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Section: Cold Responsive Gene/protein Families and Cold/freezing mentioning

confidence: 91%

Gold Responsive Gene/Protein Families and Cold/Freezing Tolerance in Cereals

Tsvetanov

Atanassov²,

Nakamura

2000

Biotechnology & Biotechnological Equipment

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“…A cDNA library was constructed from cold-acclimated seedlings of the winter-hardy common wheat cultivar M808 (Tsvetanov et al, 2000). This library was screened by plaque hybridization using the rice AOX1a cDNA (Ito et al 1997) as a probe to obtain wheat homologues.…”

Section: Methodsmentioning

confidence: 99%

Characterization of two non-homoeologous nuclear genes encoding mitochondrial alternative oxidase in common wheat.

et al. 2002

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Mitochondrial alternative oxidase (AOX) is the terminal oxidase responsible for cyanide-insensitive and salicylhydroxamic acid-sensitive respiration. We have isolated two non-homoeologous genes ( Waox1a and Waox1c ) encoding AOX proteins from common wheat ( Triticum aestivum L.). These two genes were orthologous to rice AOX1a and AOX1c , and their exon/intron structure was conserved, as it is in most other plant AOX genes. Southern blot analysis indicated that both Waox1a and Waox1c were located in at least three homoeologous loci and that additional AOX genes with lower homology were present in the genome of common wheat. The Waox1a and Waox1c loci were respectively assigned to the homoeologous group 2 and 6 chromosomes. The steady-state level of Waox1a and Waox1c transcripts increased under cold stress, while only that of Waox1a was increased by cyanide treatment.

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“…The Wcs19 transcript level is positively correlated with the relative reduction state of photosystem II in wheat (Gray et al, 1997). The WCS19 protein shows a high level of homology with another wheat member WCOR15 (Takumi et al, 2003) but a limited homology with WCOR14, an ortholog of barley BCOR14b (Tsvetanov et al, 2000). BCOR14b and WCOR14 and their nonhomologous WCS19 and WCOR15 are all encoded by the Cor/Lea genes assigned to the group 2 chromosomes of barley and common wheat (Takumi et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Improvement of freezing tolerance in tobacco plants expressing a cold-responsive and chloroplast-targeting protein WCOR15 of wheat

Shimamura

Ohno

Nakamura

et al. 2006

Journal of Plant Physiology

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Cold acclimation, an adaptive process for developing freezing tolerance in over-wintering plants, is associated with increased expression levels of a series of cold-responsive (Cor)/late embryogenesis abundant (Lea) genes. To investigate the function of Wcor15, a member of the wheat Cor/Lea gene family, for improvement of freezing tolerance, two types of transgenic tobacco lines expressing Wcor15-containing chimeric genes were produced and characterized. Immunoblot and gene expression analyses of a transgenic tobacco line expressing the Wcor15-GFP fusion gene under control of the CaMV35S promoter showed transport and abundant accumulation of the WCOR15 protein in the stromal compartment of the chloroplasts. The 5' upstream region of Wcor15 induced expression of the GFP reporter gene under low-temperature conditions in the transgenic tobacco. Both transgenic lines expressing the Wcor15-GFP fusion gene showed a similar and significantly improved level of freezing tolerance compared with the wild-type tobacco plants. Our results demonstrate that the induced expression of the wheat Wcor15 gene positively contributes to the development of freezing tolerance in the heterologous tobacco plants.

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A cold-responsive wheat (Triticum aestivum L.) gene wcor14 identified in a winter-hardy cultivar 'Mironovska 808'.

Cited by 48 publications

References 30 publications

Gold Responsive Gene/Protein Families and Cold/Freezing Tolerance in Cereals

Gold Responsive Gene/Protein Families and Cold/Freezing Tolerance in Cereals

Characterization of two non-homoeologous nuclear genes encoding mitochondrial alternative oxidase in common wheat.

Improvement of freezing tolerance in tobacco plants expressing a cold-responsive and chloroplast-targeting protein WCOR15 of wheat

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