The Molecular Biology of Photodynamic Action: Sensitized Photoautoxidations in Biological Systems ,

Spikes, John D.; Livingston, Robert

doi:10.1016/b978-1-4832-3122-8.50008-1

Cited by 196 publications

(51 citation statements)

References 340 publications

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“…The reciprocal of this constant is much smaller than the binding constant of dye to apo-cytochrome c peroxidase which can be estimated from the Scatchard plot ( 5 pM), but the second of these binding constants refers to the binding of the normal dye molecule, whereas the former presumably refers to the binding of an oxygen-activiting excited state of the dye. Similar kinetics would also be generated by obedience to the Stern-Volmer relation for concentration-dependent quenching of the dye excited state [17]. The observation that heme provides protection against photooxidation provides further evidence that modification involves the heme binding site, although the recent observations of Jori et al [18] suggest that the effect may not be simple steric blocking of the rose bengal site by heme.…”

Section: Discussjonmentioning

confidence: 88%

Interaction of Rose Bengal with Apo‐Hemoproteins

Coulson

Yonetani

1972

European Journal of Biochemistry

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The interaction of “ose bengal with apo‐hemoproteins was found to give rise to a large absorption change near 550 nm. This was used to study the binding of the dye to apo‐cytochrome c peroxidase, apo‐myoglobin, apo‐horseradish peroxidase and apo‐hemoglobin. In all cases there was about one tight and a number of weak binding sites per heme‐binding site, and the binding was impaired in the presence of stoichiometric quantities of heme. Rose bengal was found to be an effective photo‐oxidation sensitizer for apo‐cytochrome c peroxidase. Kinetic evidence was obtained consistent with the possibility that binding of the dye to the protein is an important step in the photo‐oxidation process. Heme was found to protect the enzyme from loss of enzymic activity caused by photo‐oxidation. Photo‐oxidation does not simply destroy the binding of heme to apo‐cytochrome c peroxidase, but produces changes in the spectrum of the bound heme. There is a loss of ability of recombined enzyme to form an enzyme substrate complex in the presence of stoichiometric quantities of hydrogen peroxide, which apparently causes the overall loss of enzymic activity. Photo‐oxidation was accompanied by loss of one tryptophan and two histidine residues per mole of protein, but apparently only one of the latter is essential to the enzymic activity. It was concluded that rose bengal can act as a specific modifying agent for the heme site in apo‐hemo‐proteins, and that this specificity arises from specificity of binding to the heme site. It is suggested that specificity of binding may provide a general basis for the observation of specific destruction of functionally interesting residues in photo‐oxidation.

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Section: Discussjonmentioning

confidence: 88%

Interaction of Rose Bengal with Apo‐Hemoproteins

Coulson

Yonetani

1972

European Journal of Biochemistry

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“…Virtually all of the molecular components of cell membranes are susceptible to sensitized photomodification by singlet oxygen (Foote, 1976;Spikes and Livingston, 1969). Phospholipids, proteins and cholesterol are all readily oxidized targets, while the carbohydrate moieties of the glycoproteins and glycolipids are not significantly affected.…”

Section: Biochemistry Of Singlet Oxygen Modijication Of Membranesmentioning

confidence: 99%

Photomodification of Biological Membranes With Emphasis on Singlet Oxygen Mechanisms

Valenzeno

1987

Photochem & Photobiology

263

120

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Abstract-This review discusses photomodification of biological membranes and model membrane systems. Current concepts of membrane structure are first reviewed briefly. The role of preillumination association of sensitizer with membranes as it relates to photomodification rate is discussed, as well as the role of singlet oxygen in membrane photomodification. Finally the characteristics of singlet oxygen generation in membranes are considered. The evidence clearly indicates that membrane photomodification cannot be understood based only on the properties of sensitizers and singlet oxygen in aqueous solution. Rather the properties of sensitizers in association with membranes are the determinants of membrane photomodification. These properties differ significantly in aqueous solution and in membranes.

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“…31 Von Tappeiner 32 showed that these reactions can result in the destruction of protozoa. Blum 33 and Spikes and Livingston 34 believed that PDT function depended on activated photoreactions in which the oxygen molecule takes part, resulting in photosensitized dye oxidation. There is controversy over the effect of PDT.…”

Section: Effectiveness Of Photodynamic Therapy In Treatment Of Peri-imentioning

confidence: 99%

Evaluation of Effectiveness of Photodynamic Therapy With Low-level Diode Laser in Nonsurgical Treatment of Peri-implantitis

et al. 2017

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Introduction: Side effects related to antibiotic therapy for peri-implantitis are rare in laser therapy (LT); therefore, the aim of this study was to evaluate the effectiveness of LT and photodynamic therapy (PDT) on patients with primary peri-implantitis. Methods: In this randomized clinical trial, 40 implants presenting primary peri-implantitis in 20 patients with a mean age of 52.6 years old were included using the simple sampling technique. Periodontal treatment comprising scaling and root planing (SRP) was accomplished for the whole mouth while mechanical debridement with titanium curettes and air polishing with sodium bicarbonate powder was accomplished around the implants. The implants were randomly divided into two groups and treated with LT (control) and PDT (test). The clinical indices were measured at baseline, 6 weeks and 3 months after treatment. Real-time polymerase chain reaction (PCR) was used for analysis of microbial samples at baseline and 3-month follow-up. Data were analyzed with SPSS 20, using repeated-measures analysis of variance (ANOVA) and Friedman's and MannWhitney tests (α = 0.05). Results: Both groups showed statistically significant improvements in terms of bleeding on probing (P < 0.001), probing pocket depth (PPD) (P = 0.006) and modified plaque index (P < 0.001), with no significant differences between the 2 groups (P > 0.05). The number of Aggregatibacter actinomycetemcomitans (P = 0.022), Tannerella forsythia (P = 0.038) and Porphyromonas gingivalis (P = 0.05) in the test group and Porphyromonas gingivalis (P = 0.015) in the control group significantly decreased. Conclusion:The results suggested that LT and PDT have significant short-term benefits in the treatment of primary peri-implantitis.

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The Molecular Biology of Photodynamic Action: Sensitized Photoautoxidations in Biological Systems ,

Cited by 196 publications

References 340 publications

Interaction of Rose Bengal with Apo‐Hemoproteins

Interaction of Rose Bengal with Apo‐Hemoproteins

Photomodification of Biological Membranes With Emphasis on Singlet Oxygen Mechanisms

Evaluation of Effectiveness of Photodynamic Therapy With Low-level Diode Laser in Nonsurgical Treatment of Peri-implantitis

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