1975
DOI: 10.1002/eji.1830051211
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The modulation of microprojections on the lymphocyte membrane and the redistribution of membrane‐bound ligands, a correlation

Abstract: We gratefully thank Pro6 H. lsliker for providing rabbit Fc and pep III, Dr. M. Klein for fragments of human IgC. A . Collk f o r 02microglobulin. The helpful technical assistance o f M. Cristofari and the stimulating discussions with Dr. F.M. Kourilsky were appreciated. Abbreviations: BSA: Bovine serum albumin Con A: Concanavalin A Fab: Monovalent fragments (from papain cleaved Ig) FITC: Fluoresceine isothiocyanate-conjugated IFM: Immunofluoresccnce microscopy S-: sheep-) PHA: Phytohemagglytinin RTO: Room tem… Show more

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Cited by 46 publications
(13 citation statements)
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“…One would naively expect the presence of microvilli to have at least one of two effects: (i) it might take longer for the probe to redistribute completely on a villous cell than on a smooth cell, resulting in a smaller apparent diffusion coefficient because microvilli effectively increase the total membrane area illuminated by the laser beam; (ii) recovery might be less than 100% if the lipid probe did not diffuse back up into the microvilli during the time scale of the experiment. We estimate, from published scanning electron micrographs of lymphocytes, that as much as half of the membrane surface area can be accounted for by microvilli, in agreement with other published estimates (29). The lack of a substantial effect as our cells flattened out and lost microvilli could be explained in several ways: a faster diffusion rate in the microvilli; a compensating increase in membrane viscosity as the adherent cells flatten out; or a simple failure in our intuitive expectation of the effect of microvilli on diffusion.…”
Section: Discussionsupporting
confidence: 89%
“…One would naively expect the presence of microvilli to have at least one of two effects: (i) it might take longer for the probe to redistribute completely on a villous cell than on a smooth cell, resulting in a smaller apparent diffusion coefficient because microvilli effectively increase the total membrane area illuminated by the laser beam; (ii) recovery might be less than 100% if the lipid probe did not diffuse back up into the microvilli during the time scale of the experiment. We estimate, from published scanning electron micrographs of lymphocytes, that as much as half of the membrane surface area can be accounted for by microvilli, in agreement with other published estimates (29). The lack of a substantial effect as our cells flattened out and lost microvilli could be explained in several ways: a faster diffusion rate in the microvilli; a compensating increase in membrane viscosity as the adherent cells flatten out; or a simple failure in our intuitive expectation of the effect of microvilli on diffusion.…”
Section: Discussionsupporting
confidence: 89%
“…It is well known that the lymphocyte surface is covered with numerous microvilli (41,42 (37,38). This is expected, in view of the complexity of cell surface (40).…”
Section: Methodsmentioning
confidence: 90%
“…Hammer and Apte (1992), for example, developed a model to describe a range of leukocyte-endothelial cell adhesive events such as rolling, tumbling, transient adhesion, and firm attachment, using dimensional parameters including the length and spring constants of microvilli. The values for microvillous length (Bongrand and Bell, 1984) and the number of microvilli per cell (Knutton et al, 1975;Loor and Hagg, 1975), however, were based on determinations from other cell types, and the average length of PMN microvilli was assumed to be 500 nm. This is almost twice the measured value of 290 nm determined here for PMN microvilli.…”
Section: Discussionmentioning
confidence: 99%