1982
DOI: 10.1073/pnas.79.16.4962
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Unconstrained lateral diffusion of concanavalin A receptors on bulbous lymphocytes.

Abstract: The lateral diffusion coefficient, D, of concanavalin A receptors and receptor complexes on the surface of lymphocytes and RDM4 Iymphomas is enhanced by several orders of magnitude to D > 5 x IO-9cm2/sec by induction of swelling of the cells to bulbous form. Treatments with concanavalin A or 7-nitrobenz-2-oxa-1,3-diazole-phallacidin induce blebs and the bulbous form. The resulting separation ofthe plasma membrane from most of the F-actin cytoskeleton is accompanied by release of constraints on lateral diffusio… Show more

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Cited by 71 publications
(42 citation statements)
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“…Compared to N-4-nitrobenzo-2-oxa-1,3-diazole phosphatidylethanolamine, it diffuses at a rate near the predicted theoretical limit for a freely diffusing membrane protein (32)(33)(34) . Similar diffusion coefficients have been measured for rhodopsin in amphibian rod outer segment disks (35,36), and for plasma membrane proteins in membranes in which an underlying cytoskeleton may be modified or is separated from the membrane (37)(38)(39)(40)(41)(42) . It is not known whether a membrane-associated cytoskeleton is present in the posterior tail of guinea pig sperm .…”
Section: Rapid Communicationssupporting
confidence: 74%
“…Compared to N-4-nitrobenzo-2-oxa-1,3-diazole phosphatidylethanolamine, it diffuses at a rate near the predicted theoretical limit for a freely diffusing membrane protein (32)(33)(34) . Similar diffusion coefficients have been measured for rhodopsin in amphibian rod outer segment disks (35,36), and for plasma membrane proteins in membranes in which an underlying cytoskeleton may be modified or is separated from the membrane (37)(38)(39)(40)(41)(42) . It is not known whether a membrane-associated cytoskeleton is present in the posterior tail of guinea pig sperm .…”
Section: Rapid Communicationssupporting
confidence: 74%
“…This finding should not be taken as proof that plasma membrane compartmentalization and interaction between plasma membrane components do not occur, but only that topography needs to be rejected as a plausible explanation before claiming that the diffusion is anomalous. There are several examples where cell surface smoothness has been linked to higher diffusion rates [8,11,56], supporting that convoluted and folded membranes cause an underestimation of diffusion rates when interpreted in 2D rather than 3D. Cell topography calls for a fundamental re-evaluation of the large body of work on SPTs as well as on fluorescence correlation spectroscopy (FCS).…”
Section: Avoiding Pitfalls In Data Interpretationmentioning
confidence: 99%
“…However, these numbers are of the same order of magnitude as the excess plasma membrane. For instance lymphocytes have a highly folded membrane and can be induced to double their diameter suggesting that their plasma membranes accommodate at least four times more membrane than the cell shape suggests [8]. Since diffusion coefficients are generally measured by the mean square deviation, the effect of a twofold underestimation of membrane area per pixel will result in a fourfold underestimation of the diffusion coefficient [9].…”
Section: Introductionmentioning
confidence: 99%
“…These GPMVs show simple, low-curvature geometries of giant unilamellar vesicles and appear free of cytoskeletal constraints. By using fluorophores providing recently characterized membrane fluid phase partitioning behavior (T.B., G. Hunt, E. R. Farkas, W.W.W., and G. W. Feigenson, unpublished data) for lipid phase characterization here, we find that these GPMVs can segregate into multi-micrometer-scale coexisting fluid phases, thereby enabling extension of early cell blebbing studies in our laboratories in the 1980s (15,(18)(19)(20). These results are noted and used throughout this paper.…”
mentioning
confidence: 92%