The modifier effects of chymotrypsin and trypsin enzymes on fluorescence lifetime distribution of “N-(1-pyrenyl)maleimide–bovine serum albumin” complex

Özyiğit, İbrahim Ethem; Karakuş, Emine; Pekcan, Önder

doi:10.1016/j.saa.2015.10.008

Cited by 3 publications

(1 citation statement)

References 23 publications

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“…NPM is a fluorescent reagent that is frequently utilized in the detection of derivatives of compounds containing thioamides [13]. The curve of NPM fluorescence intensity over time is presented in Figure 1.…”

Section: Reduction Of Disulfide Bonds In Defolded Lysmentioning

confidence: 99%

Mechanism of Reduced Glutathione Induced Lysozyme Defolding and Molecular Self-Assembly

Guo

Hou

Liang

et al. 2023

Foods

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The distinctive assembly behaviors of lysozyme (Lys) feature prominently in food, materials, biomedicine, and other fields and have intrigued many scholars. Although our previous work suggested that reduced glutathione (GSH) could induce lysozyme to form interfacial films at the air/water interface, the underlying mechanism is still obscure. In the present study, the effects of GSH on the disulfide bond and protein conformation of lysozyme were investigated by fluorescence spectroscopy, circular dichroism spectroscopy, and infrared spectroscopy. The findings demonstrated that GSH was able to break the disulfide bond in lysozyme molecules through the sulfhydryl/disulfide bond exchange reaction, thereby unraveling the lysozyme. The β-sheet structure of lysozyme expanded significantly, while the contents of α-helix and β-turn decreased. Furthermore, the interfacial tension and morphology analysis supported that the unfolded lysozyme tended to arrange macroscopic interfacial films at the air/water interface. It was found that pH and GSH concentrations had an impact on the aforementioned processes, with higher pH or GSH levels having a positive effect. This paper on the exploration of the mechanism of GSH-induced lysozyme interface assembly and the development of lysozyme-based green coatings has better instructive significance.

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Section: Reduction Of Disulfide Bonds In Defolded Lysmentioning

confidence: 99%