The MKK2a Gene Involved in the MAPK Signaling Cascades Enhances Populus Salt Tolerance

Wang, Jiali; Sun, Zimou; Chen, Caihui; Xu, Meng

doi:10.3390/ijms231710185

Cited by 10 publications

(8 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Prunus mume under exposure to cold, the expression of 4 PmMKK genes ( PmMKK5 , PmMKK6 , PmMKK20 , and PmMKK3 ) is reduced with prolonged treatment ( Wen et al., 2023 ). The expression patterns of MKK genes were determined in different tissues of poplar by Wang et al., and the expression of MKK2a was altered after cultivation under salt tress ( Wang et al., 2022 ). However, limited studies have explored the expression patterns of StMAPKKs under heat stress conditions.…”

Section: Discussionmentioning

confidence: 99%

StMAPKK5 responds to heat stress by regulating potato growth, photosynthesis, and antioxidant defenses

Zhu,

Li,

Zhang

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

BackgroundsAs a conserved signaling pathway, mitogen-activated protein kinase (MAPK) cascade regulates cellular signaling in response to abiotic stress. High temperature may contribute to a significant decrease in economic yield. However, research into the expression patterns of StMAPKK family genes under high temperature is limited and lacks experimental validation regarding their role in supporting potato plant growth.MethodsTo trigger heat stress responses, potato plants were grown at 35°C. qRT-PCR was conducted to analyze the expression pattern of StMAPKK family genes in potato plants. Plant with StMAPKK5 loss-of-function and gain-of-function were developed. Potato growth and morphological features were assessed through measures of plant height, dry weight, and fresh weight. The antioxidant ability of StMAPKK5 was indicated by antioxidant enzyme activity and H2O2 content. Cell membrane integrity and permeability were suggested by relative electrical conductivity (REC), and contents of MDA and proline. Photosynthetic capacity was next determined. Further, mRNA expression of heat stress-responsive genes and antioxidant enzyme genes was examined.ResultsIn reaction to heat stress, the expression profiles of StMAPKK family genes were changed. The StMAPKK5 protein is located to the nucleus, cytoplasm and cytomembrane, playing a role in controlling the height and weight of potato plants under heat stress conditions. StMAPKK5 over-expression promoted photosynthesis and maintained cell membrane integrity, while inhibited transpiration and stomatal conductance under heat stress. Overexpression of StMAPKK5 triggered biochemical defenses in potato plant against heat stress, modulating the levels of H2O2, MDA and proline, as well as the antioxidant activities of CAT, SOD and POD. Overexpression of StMAPKK5 elicited genetic responses in potato plants to heat stress, affecting heat stress-responsive genes and genes encoding antioxidant enzymes.ConclusionStMAPKK5 can improve the resilience of potato plants to heat stress-induced damage, offering a promising approach for engineering potatoes with enhanced adaptability to challenging heat stress conditions.

show abstract

Section: Discussionmentioning

confidence: 99%

StMAPKK5 responds to heat stress by regulating potato growth, photosynthesis, and antioxidant defenses

Zhu,

Li,

Zhang

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…Studies showed that MAPK signaling could occur in response to plant cell differentiation and development, maturation, hormone signal transduction, and immune processes via phosphorylation of multiple transcription factors and other signaling pathway components (Wang et al 2022b ; de Zelicourt et al 2016 ; Danquah et al 2014 ). Meanwhile, MAPK protein kinases also affect intracellular responses and functions under biotic and abiotic stresses (Danquah et al 2014 ; Jagodzik et al 2018 ).…”

Section: Discussionmentioning

confidence: 99%

“…It has been found that plants, in response to extracellular abiotic stress, activate a complex intercellular signaling cascade that regulates physiological and biochemical changes. The Mitogen-activated protein kinase (MAPK) cascades consist of three layers of sequentially phosphorylating and activating protein kinases, including MAPK kinase kinases (MAPKKKs), MAPK kinases (MAPKKs), and MAPKs, which are highly conserved eukaryotic signaling modules acting downstream of the receptors in transducing extracellular stimuli into cellular responses (Wang et al 2022b , 2014 ; Jia et al 2022 ; Liao et al 2021 ). There are 17 MAPKs, 8 MAPKKs, and 75 MAPKKKs in the rice genome, which play crucial roles in the transduction of environmental and developmental signals, and response to various stresses (Wankhede et al 2013 ; Singh et al 2014 ; Hamel et al 2006 ; Jagodzik et al 2018 ).…”

Section: Introductionmentioning

confidence: 99%

OsSTS, a Novel Allele of Mitogen-Activated Protein Kinase Kinase 4 (OsMKK4), Controls Grain Size and Salt Tolerance in Rice

Liu,

Shen,

Guo

et al. 2023

Rice

View full text Add to dashboard Cite

Soil salinization is one of the most common abiotic stresses of rice, which seriously affects the normal growth of rice. Breeding salt-tolerant varieties have become one of the important ways to ensure food security and sustainable agricultural development. However, the mechanisms underlying salt tolerance control still need to be clarified. In this study, we identified a mutant, termed salt-tolerant and small grains(sts), with salt tolerance and small grains. Gene cloning and physiological and biochemical experiments reveal that sts is a novel mutant allele of Mitogen-activated protein Kinase Kinase 4 (OsMKK4), which controls the grain size, and has recently been found to be related to salt tolerance in rice. Functional analysis showed that OsSTS is constitutively expressed throughout the tissue, and its proteins are localized to the nucleus, cell membrane, and cytoplasm. It was found that the loss of OsSTS function enhanced the salt tolerance of rice seedlings, and further studies showed that the loss of OsSTS function increased the ROS clearance rate of rice seedlings, independent of ionic toxicity. In order to explore the salt tolerance mechanism of sts, we found that the salt tolerance of sts is also regulated by ABA through high-throughput mRNA sequencing. Salt and ABA treatment showed that ABA might alleviate the inhibitory effect of salt stress on root length in sts. These results revealed new functions of grain size gene OsMKK4, expanded new research ideas related to salt tolerance mechanism and hormone regulation network, and provided a theoretical basis for salt-tolerant rice breeding.

show abstract

“…In poplar, overexpression of PeMPK7 alleviated the phenotypic injury induced by para-hydroxybenzoic acid stress (Zhao et al, 2022). Moreover, overexpression of the PtMKK4 gene significantly enhances the tolerance to drought stress (Wang et al, 2014b) and overexpressing PeMKK2a enhanced salt tolerance in poplar (Wang et al, 2022).…”

Section: Introductionmentioning

confidence: 97%

“…In poplar, overexpression of PeMPK7 alleviated the phenotypic injury induced by para ‐hydroxybenzoic acid stress (Zhao et al, 2022). Moreover, overexpression of the PtMKK4 gene significantly enhances the tolerance to drought stress (Wang et al, 2014b) and overexpressing PeMKK2a enhanced salt tolerance in poplar (Wang et al, 2022). Deregulation of MPK4 in poplar can improve temperature and acclimatization by affecting stomatal movement and leaf development, including increased tolerance to sudden temperature changes or excessive light intensity (Witon et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Comprehensive analysis of MAPK gene family in Populus trichocarpa and physiological characterization of PtMAPK3‐1 in response to MeJA induction

Liang

Lin

et al. 2023

Physiologia Plantarum

View full text Add to dashboard Cite

Mitogen‐activated protein kinases (MAPKs) play important roles in plant growth and development, as well as hormone and stress responses by signaling to eukaryotic cells, through MAPK cascade, the presence of various cues; thereby, regulating various responses. The MAPK cascade consists mainly of three gene families, MAPK, MAPKK, and MAPKKK, which activate downstream signaling pathways through sequential phosphorylation. Although the MAPK cascade gene family has been reported in several species, there is a lack of comprehensive analysis in poplar. We identified 21 MAPK genes, 11 MAPKK genes, and 104 MAPKKK genes in Populus trichocarpa. The phylogenetic classification was supported by conservative motif, gene structure and motif analysis. Whole genome duplication has an important role in the expansion of MAPK cascade genes. Analysis of promoter cis‐elements and expression profiles indicates that MAPK cascade genes have important roles in plant growth and development, abiotic and biotic stresses, and phytohormone response. Expression profiling revealed a significant upregulation of PtMAPK3‐1 expression in response to drought, salt and disease stresses. Poplar transiently overexpressing PtMAPK3‐1 and treated with methyl jasmonic acid (MeJA) had higher catalase and peroxidase levels than non‐overexpressing poplar. This work represents the first complete inventory of the MAPK cascade in P. trichocarpa, which reveals that PtMAPK3‐1 is induced by the MeJA hormone and participates in the MeJA‐induced enhancement of the antioxidant enzyme system.

show abstract

The MKK2a Gene Involved in the MAPK Signaling Cascades Enhances Populus Salt Tolerance

Cited by 10 publications

References 57 publications

StMAPKK5 responds to heat stress by regulating potato growth, photosynthesis, and antioxidant defenses

StMAPKK5 responds to heat stress by regulating potato growth, photosynthesis, and antioxidant defenses

OsSTS, a Novel Allele of Mitogen-Activated Protein Kinase Kinase 4 (OsMKK4), Controls Grain Size and Salt Tolerance in Rice

Comprehensive analysis of MAPK gene family in Populus trichocarpa and physiological characterization of PtMAPK3‐1 in response to MeJA induction

Contact Info

Product

Resources

About