The mitochondrial DNA content of cumulus granulosa cells is linked to embryo quality

Desquiret-Dumas, Valérie; Clément, Arthur; Seegers, Valérie; Boucret, Lisa; Ferré-L’Hôtellier, Véronique; Bouet, Pierre‐Emmanuel; Descamps, Philippe; Procaccio, Vincent; Reynier, Pascal; May‐Panloup, Pascale

doi:10.1093/humrep/dew341

Cited by 53 publications

(54 citation statements)

References 35 publications

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“…Recent studies have proposed quantifying mtDNA in cumulus, granulosa and trophectoderm cells to predict embryo quality and viability (Desquiret-Dumas et al, 2017;Diez-Juan et al, 2015;Fragouli et al, 2015;Ogino et al, 2016). Boucret et al (2015) concluded that the mtDNA content in cumulus cells is correlated with the amount detected within the oocyte for each cumulus-oocyte complex.…”

Section: Biogenesis Of Mtdna and Copy Numbermentioning

confidence: 99%

The role of mitochondrial activity in female fertility and assisted reproductive technologies: overview and current insights

Cecchino

Seli

Motta

et al. 2018

Reproductive BioMedicine Online

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Mitochondria have been implicated as key factors regulating female reproductive processes. Notable progress has been made in determining the role of mitochondria with respect to oocyte maturation, fertilization and early embryo development. In addition, mitochondrial function and dysfunction has been the subject of various studies in ovarian ageing and metabolic stress models. However, the overall mitochondrial impact on female fertility is yet to be uncovered. The mitochondrial DNA content of granulosa, cumulus and trophectoderm cells is being explored as a biomarker of oocyte quality and embryo viability. As growing evidence suggests that embryo potential could be related to the ability of oocyte mitochondria to generate energy, efforts have been made to investigate the possibility of improving mitochondrial capacity in women with poor outcomes after treatment with assistedreproductive technologies. Thus far, therapeutic attempts have focused mainly on using nutrients to restore mitochondrial function and transferring mitochondria from autologous germline precursor cells. Moreover, new perspectives on optimizing infertility treatments have arisen with modern mitochondrial replacement therapies, which are being applied in women with mitochondrial disease-causing mutations. This review explores aspects of the distinctive contribution of mitochondria to reproductive processes and discusses current and emerging clinical implications.

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Section: Biogenesis Of Mtdna and Copy Numbermentioning

confidence: 99%

The role of mitochondrial activity in female fertility and assisted reproductive technologies: overview and current insights

Cecchino

Seli

Motta

et al. 2018

Reproductive BioMedicine Online

View full text Add to dashboard Cite

show abstract

“…To the authors’ knowledge, this study is the first to report that FF impacts mitochondrial biogenesis in cumulus cells, but molecular events underlying the increase in biogenesis is a subject for further experiments. Nevertheless, studies have shown that the mtDNA copy number in cumulus cells reflects a high yield of good embryos in humans . Thus, a beneficial effect of FF on oocyte developmental competence is associated with an increased mtDNA in cumulus cells.…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, studies have shown that the mtDNA copy number in cumulus cells reflects a high yield of good embryos in humans. 26,27 Thus, a beneficial effect of FF on oocyte developmental competence is associated with an increased mtDNA in cumulus cells.…”

Section: Discussionmentioning

confidence: 99%

Non‐esterified fatty acid‐associated ability of follicular fluid to support porcine oocyte maturation and development

Ogawa

Itami

Ueda

et al. 2018

Reprod Medicine & Biology

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PurposeThe effect of supplementing maturation medium with follicular fluid (FF) was examined according to its non‐esterified fatty acid (NEFA) content or with a fatty acid mixture (FA‐Mix) on the developmental competence of oocytes, as well as the mitochondrial quality and quantity in the oocytes and cumulus cells.MethodPorcine oocytes from a slaughterhouse were used.ResultsThe FF or FA‐Mix in maturation medium increased the lipid content in both the oocytes and the cumulus cells, but the adenosine triphosphate content was differentially affected. The FF supplementation increased the mitochondrial DNA copy number, survival of cumulus cells, and rate of oocyte development to the blastocyst stage, whereas the FA‐Mix supplementation did not show these effects. The expression levels of GPC4,PFKP,PRDX3, and TFAM in the cumulus cells increased after FF supplementation, but the expression of GJA1 decreased, compared with the cells that were cultured without FF.ConclusionAdding FF and FA‐Mix to the maturation medium increased the lipid content in the oocytes and cumulus cells. The effects of FF on the cumulus cells and oocytes were not observed after FA‐Mix supplementation, indicating that the concentration of the NEFAs in the FF are closely associated with an ability to support oocyte maturation and the metabolism of cumulus cells and oocytes.

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“…In healthy embryos, the accumulated mtDNA is divided equally among all cells during embryogenesis . Recent studies have proposed quantification of mtDNA in cumulus, granulosa, and trophectoderm cells as a promising strategy for predicting embryo quality and viability . Since mtDNA content in cumulus cells is correlated with that in oocytes for each cumulus‐oocyte complex, it is suggested that the mitochondrial characteristics of the cells may serve as a marker of the oocyte quality .…”

Section: Factors Influencing Oocyte Qualitymentioning

confidence: 99%

“…[61][62][63][64] Recent studies have proposed quantification of mtDNA in cumulus, granulosa, and trophectoderm cells as a promising strategy for predicting embryo quality and viability. 62,65 Since mtDNA content in cumulus cells is correlated with that in oocytes for each cumulus-oocyte complex, it is suggested that the mitochondrial characteristics of the cells may serve as a marker of the oocyte quality. 40 Furthermore, mutations or deletions in mtDNA have been correlated with organelle dysfunction, low ATP levels, and embryonic developmental arrest.…”

Section: Mitochondriamentioning

confidence: 99%

Updating the markers for oocyte quality evaluation: intracellular temperature as a new index

Hoshino

2018

Reprod Medicine & Biology

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BackgroundThe developmental competence of an embryo is principally dictated by the oocyte. Usually, oocyte selection is based on morphological properties; however, all morphological criteria that are currently used for the grading and screening of oocytes are not able to eliminate the subjectivity. Despite recent studies of the molecular factors related to oocyte quality, it is technically difficult to develop an index based on these factors, and new indices that reflect intracellular conditions are necessary.MethodsMorphological and molecular factors influencing developmental competence were comprehensively reviewed, and intracellular temperature was evaluated as a new marker of oocyte quality.Main findingsThe intracellular temperature of mature oocytes was high in fresh oocytes and decreased with time after polar body release. Under the same conditions, the intracellular temperature and its distribution differed among oocytes, suggesting that temperature represents the state of each oocyte.ConclusionIntracellular temperature is advantageous as an objective and quantitative indicator of oocyte quality. Further studies should evaluate the link between temperature and cellular phenomena to establish its use as an indicator of quality.

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The mitochondrial DNA content of cumulus granulosa cells is linked to embryo quality

Abstract: This work was supported by the University Hospital of Angers, the University of Angers, France, and the French national research centres INSERM and the CNRS. There were no competing interests.

Cited by 53 publications

References 35 publications

The role of mitochondrial activity in female fertility and assisted reproductive technologies: overview and current insights

The role of mitochondrial activity in female fertility and assisted reproductive technologies: overview and current insights

Non‐esterified fatty acid‐associated ability of follicular fluid to support porcine oocyte maturation and development

Updating the markers for oocyte quality evaluation: intracellular temperature as a new index

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