The miR159‐MYB33‐ABI5 module regulates seed germination in <i>Arabidopsis</i>

Jiang, Youqi; Wu, Xi; Shi, Min; Yu, Jing; Guo, Changkui

doi:10.1111/ppl.13659

Cited by 18 publications

(13 citation statements)

References 55 publications

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“…The upstream region of MIR159 was analyzed, revealing the presence of ABRE response elements. Previous studies have shown that the application of exogenous ABA can induce the expression of miR159 and inhibit the expression of MYB33 , reducing the sensitivity of plants to ABA, which plays a vital regulatory role in the transformation process of seed germination and breaking dormancy (Achard et al., 2004; Guo et al., 2017; Jiang et al., 2022; Reyes & Chua, 2007). Overexpression of MYB33 and MYB101 has been observed to result in hypersensitivity to ABA in Arabidopsis and tomato (Hou et al., 2020).…”

Section: Discussionmentioning

confidence: 99%

The miR159a‐PeMYB33 module regulates poplar adventitious rooting through the abscisic acid signal pathway

Zhao,

Lei,

et al. 2024

The Plant Journal

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SUMMARYAs sessile organisms, plants experience variable environments and encounter diverse stresses during their growth and development. Adventitious rooting, orchestrated by multiple coordinated signaling pathways, represents an adaptive strategy evolved by plants to adapt to cope with changing environmental conditions. This study uncovered the role of the miR159a‐PeMYB33 module in the formation of adventitious roots (ARs) synergistically with abscisic acid (ABA) signaling in poplar. Overexpression of miR159a increased the number of ARs and plant height while reducing sensitivity to ABA in transgenic plants. In contrast, inhibition of miR159a (using Short Tandem Target Mimic) or overexpression of PeMYB33 decreased the number of ARs in transgenic plants. Additionally, miR159a targets and cleaves transcripts of PeMYB33 using degradome analysis, which was further confirmed by a transient expression experiment of poplar protoplast. We show the miR159a‐PeMYB33 module controls ARs development in poplar through ABA signaling. In particular, we demonstrated that miR159a promotes the expression of genes in the ABA signaling pathway. The findings from this study shed light on the intricate regulatory mechanisms governing the development of ARs in poplar plants. The miR159a‐PeMYB33 module, in conjunction with ABA signaling, plays a crucial role in modulating AR formation and subsequent plant growth.

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Section: Discussionmentioning

confidence: 99%

The miR159a‐PeMYB33 module regulates poplar adventitious rooting through the abscisic acid signal pathway

Zhao,

Lei,

et al. 2024

The Plant Journal

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“…ABI5 acts as a central transcription factor and marker that negatively regulates drought stress in Arabidopsis and rice (Zou et al, 2008; Collin et al, 2020; Ju et al, 2020; Li et al, 2021; Wang et al, 2021; Jiang et al, 2022). In Brachypodium distachyon , HD1‐ OE plants showed improved tolerance to drought stress by reducing the expression of ABI5 (Song et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Overexpression of TaNCED1 from wheat ( Triticum aestivum L.) resulted in higher drought tolerance in tobacco ( Nicotiana tabacum L.) (Zhang et al, 2014). ABA‐INSENSITIVE 5 ( ABI5 ) is a central ABA‐responsive gene that negatively regulates drought stress in plants (Zou et al, 2008; Collin et al, 2020; Ju et al, 2020; Li et al, 2021; Wang et al, 2021; Jiang et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

OsWR2 recruits HDA704 to regulate the deacetylation of H4K8ac in the promoter of OsABI5 in response to drought stress

Guo

Tan

et al. 2023

JIPB

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Drought stress is a major environmental factor that limits the growth, development, and yield of rice (Oryza sativa L.). Histone deacetylases (HDACs) are involved in the regulation of drought stress responses. HDA704 is an RPD3/HDA1 class HDAC that mediates the deacetylation of H4K8 (lysine 8 of histone H4) for drought tolerance in rice. In this study, we show that plants overexpressing HDA704 (HDA704-OE) are resistant to drought stress and sensitive to abscisic acid (ABA), whereas HDA704 knockout mutant (hda704) plants displayed decreased drought tolerance and ABA sensitivity. Transcriptome analysis revealed that HDA704 regulates the expression of ABA-related genes in response to drought stress. Moreover, HDA704 was recruited by a drought-resistant transcription factor, WAX SYNTHESIS REGULATORY 2 (OsWR2), and co-regulated the expression of the ABA biosynthesis genes NINE-CIS-EPOXYCAROTENOID DIOX-YGENASE 3 (NCED3), NCED4, and NCED5 under drought stress. HDA704 also repressed the expression of ABA-INSENSITIVE 5 (OsABI5) and DWARF AND SMALL SEED 1 (OsDSS1) by regulating H4K8ac levels in the promoter regions in response to polyethylene glycol 6000 treatment. In agreement, the loss of OsABI5 function increased resistance to dehydration stress in rice. Our results demonstrate that HDA704 is a positive regulator of the drought stress response and offers avenues for improving drought resistance in rice.

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“…The role of MYB 33/101 TFs in ABA signaling has already been proven for seed germination in the case of Arabidopsis (Reyes & Chua, 2007 ). Recently in Arabidopsis , the role of mir159‐MYB33‐ABI5 in seed germination and drought response has been described (Guo et al, 2021 ; Jiang et al, 2022 ). However, the role of these TFs and MYB65 in the drought response has never been tested.…”

Section: Discussionmentioning

confidence: 99%

The MYB33, MYB65, and MYB101 transcription factors affect Arabidopsis and potato responses to drought by regulating the ABA signaling pathway

Wyrzykowska

Bielewicz

Plewka

et al. 2022

Physiologia Plantarum

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Drought is one of the main climate threats limiting crop production. Potato is one of the four most important food crop species worldwide and is sensitive to water shortage. The CBP80 gene was shown to affect Arabidopsis and potato responses to drought by regulating the level of microRNA159 and, consequently, the levels of the MYB33 and MYB101 transcription factors (TFs). Here, we show that three MYB TFs, MYB33, MYB65, and MYB101, are involved in plant responses to water shortage.Their downregulation in Arabidopsis causes stomatal hyposensitivity to abscisic acid (ABA), leading to reduced tolerance to drought. Transgenic Arabidopsis and potato plants overexpressing these genes, with a mutated recognition site in miR159, show hypersensitivity to ABA and relatively high tolerance to drought conditions. Thus, the MYB33, MYB65, and MYB101 genes may be potential targets for innovative breeding to obtain crops with relatively high tolerance to drought.

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The miR159‐MYB33‐ABI5 module regulates seed germination in Arabidopsis

Cited by 18 publications

References 55 publications

The miR159a‐PeMYB33 module regulates poplar adventitious rooting through the abscisic acid signal pathway

The miR159a‐PeMYB33 module regulates poplar adventitious rooting through the abscisic acid signal pathway

OsWR2 recruits HDA704 to regulate the deacetylation of H4K8ac in the promoter of OsABI5 in response to drought stress

The MYB33, MYB65, and MYB101 transcription factors affect Arabidopsis and potato responses to drought by regulating the ABA signaling pathway

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