The <scp>MYB33</scp>, <scp>MYB65</scp>, and <scp>MYB101</scp> transcription factors affect <i>Arabidopsis</i> and potato responses to drought by regulating the <scp>ABA</scp> signaling pathway

Wyrzykowska, Anna; Bielewicz, Dawid; Plewka, Patrycja; Sołtys‐Kalina, Dorota; Wasilewicz‐Flis, Iwona; Marczewski, Waldemar; Jarmołowski, Artur; Szweykowska-Kulińska, Zofia

doi:10.1111/ppl.13775

Cited by 19 publications

(9 citation statements)

References 67 publications

(108 reference statements)

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“…The expression of 25 TFs including ve AP2/ERF, ve NAC, eight RING-type zinc ngers were induced after 18 days of drought treatment (Table S5), suggesting their potential importance for drought tolerance in white spruce. The two MYB sequences identi ed in this study had homologies with putative Arabidopsis thaliana proteins known to enhance protection against oxidative damage or to be involved in growth, phenylpropanoid biosynthesis, and the ABA signaling pathway [127][128][129]. Drought-induced AP2/ERF genes in our study were close homologs to ethylene responsive elements in other species [130].…”

Section: Drought-responsive Genes Coding For Protective Defense and S...mentioning

confidence: 88%

De novo transcriptome assembly and discovery of drought-responsive genes in eastern white spruce (Picea glauca)

Ribeyre,

Depardieu,

Prunier

et al. 2024

Preprint

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Background Forests face an escalating threat from the increasing frequency of extreme drought events driven by climate change. To address this challenge, it is crucial to understand how widely distributed species of economic or ecological importance may respond to drought stress. Here, we used RNA-sequencing to investigate transcriptome responses at increasing levels of water stress in white spruce (Picea glauca (Moench) Voss), distributed across North America. We began by generating a transcriptome assembly emphasizing short-term drought stress at different developmental stages. We also analyzed differential gene expression at four time points over 22 days in a controlled drought stress experiment involving 2-year-old plants and three genetically unrelated clones. Results De novo transcriptome assembly and gene expression analysis revealed a total of 33,287 transcripts (18,934 annotated unique genes), with 4,425 unique drought-responsive genes. Many transcripts that had predicted functions associated with photosynthesis, cell wall organization, and water transport were down-regulated under drought conditions, while transcripts linked to abscisic acid response and defense response were up-regulated. Our study highlights a previously uncharacterized effect of drought stress on lipid metabolism genes in conifers and significant changes in the expression of several transcription factors, suggesting a regulatory response potentially linked to drought response or acclimation. Conclusion Our research represents a fundamental step in unraveling the molecular mechanisms underlying short-term drought responses in white spruce seedlings. In addition, it provides a valuable source of new genetic data that could contribute to genetic selection strategies aimed at enhancing the drought resistance and resilience of white spruce to changing climates.

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Section: Drought-responsive Genes Coding For Protective Defense and S...mentioning

confidence: 88%

De novo transcriptome assembly and discovery of drought-responsive genes in eastern white spruce (Picea glauca)

Ribeyre,

Depardieu,

Prunier

et al. 2024

Preprint

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“…It is worth noting that, despite the negative impact of PeMYB33 on growth and development, it may play an important role in responding to both biotic and abiotic stress. Research shows that drought treatment enhances stomatal closure in OE‐MYB33 plants, thereby increasing their drought tolerance (Wyrzykowska et al., 2022). Furthermore, MYB33 can bind to the LEAFY promoter, promoting its expression, and thus influencing the flowering time (Achard et al., 2004).…”

Section: Discussionmentioning

confidence: 99%

The miR159a‐PeMYB33 module regulates poplar adventitious rooting through the abscisic acid signal pathway

Zhao,

Lei,

et al. 2024

The Plant Journal

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SUMMARYAs sessile organisms, plants experience variable environments and encounter diverse stresses during their growth and development. Adventitious rooting, orchestrated by multiple coordinated signaling pathways, represents an adaptive strategy evolved by plants to adapt to cope with changing environmental conditions. This study uncovered the role of the miR159a‐PeMYB33 module in the formation of adventitious roots (ARs) synergistically with abscisic acid (ABA) signaling in poplar. Overexpression of miR159a increased the number of ARs and plant height while reducing sensitivity to ABA in transgenic plants. In contrast, inhibition of miR159a (using Short Tandem Target Mimic) or overexpression of PeMYB33 decreased the number of ARs in transgenic plants. Additionally, miR159a targets and cleaves transcripts of PeMYB33 using degradome analysis, which was further confirmed by a transient expression experiment of poplar protoplast. We show the miR159a‐PeMYB33 module controls ARs development in poplar through ABA signaling. In particular, we demonstrated that miR159a promotes the expression of genes in the ABA signaling pathway. The findings from this study shed light on the intricate regulatory mechanisms governing the development of ARs in poplar plants. The miR159a‐PeMYB33 module, in conjunction with ABA signaling, plays a crucial role in modulating AR formation and subsequent plant growth.

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“…In our study, hypo-DMR-associated upregulated DEGs that might be regulated by TFs were identified, including MYB101 ( AT2G32460 ) and ATGH3.12 ( AT5G13320 ). Previous research indicates that MYB101 functions as a constructive regulatory element in ABA-stimulated growth cessation, and an ineffective MYB101 mutant reduces the sensitivity of plants to ABA [ 43 , 44 ]. It has been observed that ABA has the potential to induce senescence in leaves through a pathway that is independent of ethylene.…”

Section: Discussionmentioning

confidence: 99%

The Change in Whole-Genome Methylation and Transcriptome Profile under Autophagy Defect and Nitrogen Starvation

Shi,

Yu,

Cheng

et al. 2023

IJMS

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Through whole-genome bisulfite sequencing and RNA-seq, we determined the potential impact of autophagy in regulating DNA methylation in Arabidopsis, providing a solid foundation for further understanding the molecular mechanism of autophagy and how plants cope with nitrogen deficiency. A total of 335 notable differentially expressed genes (DEGs) were discovered in wild-type Arabidopsis (Col-0-N) and an autophagic mutant cultivated under nitrogen starvation (atg5-1-N). Among these, 142 DEGs were associated with hypomethylated regions (hypo-DMRs) and were upregulated. This suggests a correlation between DNA demethylation and the ability of Arabidopsis to cope with nitrogen deficiency. Examination of the hypo-DMR-linked upregulated DEGs indicated that the expression of MYB101, an ABA pathway regulator, may be regulated by DNA demethylation and the recruitment of transcription factors (TFs; ERF57, ERF105, ERF48, and ERF111), which may contribute to the growth arrest induced by abscisic acid (ABA). Additionally, we found that DNA methylation might impact the biosynthesis of salicylic acid (SA). The promoter region of ATGH3.12 (PBS3), a key enzyme in SA synthesis, was hypomethylated, combined with overexpression of PBS3 and its potential TF AT3G46070, suggesting that autophagy defects may lead to SA-activated senescence, depending on DNA demethylation. These findings suggest that DNA hypomethylation may impact the mechanism by which Arabidopsis autophagy mutants (atg5-1) respond to nitrogen deficiency, specifically in relation to ABA and SA regulation. Our evaluation of hormone levels verified that these two hormones are significantly enriched under nitrogen deficiency in atg5-1-N compared to Col-0-N.

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The MYB33, MYB65, and MYB101 transcription factors affect Arabidopsis and potato responses to drought by regulating the ABA signaling pathway

Cited by 19 publications

References 67 publications

De novo transcriptome assembly and discovery of drought-responsive genes in eastern white spruce (Picea glauca)

De novo transcriptome assembly and discovery of drought-responsive genes in eastern white spruce (Picea glauca)

The miR159a‐PeMYB33 module regulates poplar adventitious rooting through the abscisic acid signal pathway

The Change in Whole-Genome Methylation and Transcriptome Profile under Autophagy Defect and Nitrogen Starvation

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