The miR-145–MMP1 axis is a critical regulator for imiquimod-induced cancer stemness and chemoresistance

Zhu, Shan; Yang, Ning; Niu, Chao; Wang, Wan; Wang, Xue; Bai, Junge; Qiao, Yuan; Deng, Shuanglin; Guan, Yunqian; Chen, Jingtao

doi:10.1016/j.phrs.2022.106196

Cited by 7 publications

(5 citation statements)

References 44 publications

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“…The gene silencing caused by aberrant promoter methylation has been demonstrated in oncogenesis and progression, which showed the potential as a prognostic or therapeutic biomarker. [25][26][27] The present study identified that the promoter methylation levels of CSF3R…”

Section: Discussionmentioning

confidence: 50%

Integrated transcriptome, proteome and single‐cell sequencing uncover the prognostic and immunological features of colony‐stimulating factor 3 receptor in pan‐cancer

Song

Jiang

Tian

et al. 2023

The Journal of Gene Medicine

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BackgroundColony‐stimulating factor 3 receptor (CSF3R) has been demonstrated to be associated with various hematological tumors, especially chronic neutrophilic leukemia; however, the detailed roles of CSF3R in other cancers remain to be explored.MethodsIn the present study, we systematically analyzed the expression profiles of CSF3R in pan‐cancer by comprehensive bioinformatics databases, such as Tumor Immune Estimation Resource, version 2 (TIMER2.0), Gene Expression Profiling Interactive Analysis, version 2 (GEPIA2.0), etc. GEPIA2.0 was also used to analyze the relationship between CSF3R expression and patients' survival prognosis.ResultsWe found that the high expression of CSF3R was associated with a poor prognosis in the brain tumor patients, such as brain lower grade glioma and glioblastoma multiforme. In addition, we further investigated the genetic mutation and DNA methylation level of CSF3R in multiple cancers. Immune infiltration analysis showed that CSF3R expression was positively correlated with a variety of tumor‐infiltrating immune cells in most cancers. Single cell sequencing indicated that CSF3R levels were correlated with several cancer‐associated pathways, such as DNA damage, cell invasion, and stemness.ConclusionsTaken together, the role of CSF3R in multiple cancers might reveal its potential as a novel prognostic biomarker and therapeutic target for cancer patients.

show abstract

Section: Discussionmentioning

confidence: 50%

Integrated transcriptome, proteome and single‐cell sequencing uncover the prognostic and immunological features of colony‐stimulating factor 3 receptor in pan‐cancer

Song

Jiang

Tian

et al. 2023

The Journal of Gene Medicine

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“… 40 , 49 Moreover, LGR6 contributes to CSC properties in ovarian, breast, and cervical cancers. 22 , 23 , 24 In our study, when comparing LGR6 expression between spheroid and adherent components of A427 cells harboring a CTNNB1 exon 3 mutation, LGR6 was predominantly expressed in the component of spheroids, which is a CSC‐related characteristic, 38 accompanied by upregulation of several stem cell‐associated pathways and genes, including MMP1 , 50 CPA4 , 51 and SRPX2 . 52 These findings support the idea that Wnt/β‐catenin pathway activation, mediated by CTNNB1 exon 3 mutations, induces LGR6 overexpression, which in turn confers cancer stemness properties via Wnt pathway activation.…”

Section: Discussionmentioning

confidence: 66%

The oncogenic role of LGR6 overexpression induced by aberrant Wnt/β‐catenin signaling in lung cancer

Sunaga,

Kaira,

Shimizu

et al. 2023

Thoracic Cancer

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BackgroundMolecular abnormalities in the Wnt/β‐catenin pathway confer malignant phenotypes in lung cancer. Previously, we identified the association of leucine‐rich repeat‐containing G protein‐coupled receptor 6 (LGR6) with oncogenic Wnt signaling, and its downregulation upon β‐catenin knockdown in non‐small cell lung cancer (NSCLC) cells carrying CTNNB1 mutations. The aim of this study was to explore the mechanisms underlying this association and the accompanying phenotypes.MethodsLGR6 expression in lung cancer cell lines and surgical specimens was analyzed using quantitative RT‐PCR and immunohistochemistry. Cell growth was assessed using colony formation assay. Additionally, mRNA sequencing was performed to compare the expression profiles of cells subjected to different treatments.ResultsLGR6 was overexpressed in small cell lung cancer (SCLC) and NSCLC cell lines, including the CTNNB1‐mutated NSCLC cell lines HCC15 and A427. In both cell lines, LGR6 knockdown inhibited cell growth. LGR6 expression was upregulated in spheroids compared to adherent cultures of A427 cells, suggesting that LGR6 participates in the acquisition of cancer stem cell properties. Immunohistochemical analysis of lung cancer specimens revealed that the LGR6 protein was predominantly overexpressed in SCLCs, large cell neuroendocrine carcinomas, and lung adenocarcinomas, wherein LGR6 overexpression was associated with vascular invasion, the wild‐type EGFR genotype, and an unfavorable prognosis. Integrated mRNA sequencing analysis of HCC15 and A427 cells with or without LGR6 knockdown revealed LGR6‐related pathways and genes associated with cancer development and stemness properties.ConclusionsOur findings highlight the oncogenic roles of LGR6 overexpression induced by aberrant Wnt/β‐catenin signaling in lung cancer.

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“…The neuronal marker genes NES, MAP2, PTK2B, CHN1, DNM1, NRSN2, FBLN2, and SCAMP1 [ 24 , [28] , [29] , [30] , [31] , [32] , [33] , [34] ] gradually increased with the induction time, while TMEM59L, SLC1A1, DLG4, CDK5, and ENO2 [ [35] , [36] , [37] , [38] , [39] ] peaked in the induction 5h cell cluster but significantly decreased in the induction 8h cell cluster. However, the expression of CCND1, IL1B, MMP1, MMP3, MYO10, and BMP2 [ [40] , [41] , [42] , [43] , [44] , [45] , [46] ], stem cell marker genes, were highest in the ADSC cluster and significantly decreased in their differentiated cell clusters with increasing induction time. Meanwhile, the BP functions related to neuronal differentiation and development, such as neuronal differentiation and projection and apoptosis, dendritic morphogenesis, axonogenesis, guidance, extension, protein transport and damage response and regeneration, synaptic assembly, cell transport and regulation of endocytosis in their differentiated cell clusters were significantly up-regulated with longer induction time.…”

Section: Discussionmentioning

confidence: 99%

Genomic characteristics of adipose-derived stromal cells induced into neurons based on single-cell RNA sequencing

Yuan,

Li,

Liu

et al. 2024

Heliyon

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The miR-145–MMP1 axis is a critical regulator for imiquimod-induced cancer stemness and chemoresistance

Cited by 7 publications

References 44 publications

Integrated transcriptome, proteome and single‐cell sequencing uncover the prognostic and immunological features of colony‐stimulating factor 3 receptor in pan‐cancer

Integrated transcriptome, proteome and single‐cell sequencing uncover the prognostic and immunological features of colony‐stimulating factor 3 receptor in pan‐cancer

The oncogenic role of LGR6 overexpression induced by aberrant Wnt/β‐catenin signaling in lung cancer

Genomic characteristics of adipose-derived stromal cells induced into neurons based on single-cell RNA sequencing

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