The Mink Circovirus Capsid Subunit Expressed by Recombinant Baculovirus Protects Minks against Refractory Diarrhea in Field

Wang, Lidong; Zhang, Yanyan; Chen, Teng; Mi, Ling; Sun, Xiaohong; Zhou, Xintao; Miao, Faming; Zhang, Shoufeng; Liu, Ye; Hu, Rongliang

doi:10.3390/v13040606

Cited by 3 publications

(6 citation statements)

References 23 publications

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“…The results showed that the sensitivity of qPCR was higher than that of conventional PCR to detect MiCV. Moreover, the minimum detection limit of the qPCR method established in this study was lower than that of the previously established conventional PCR method and qPCR method ( Wang et al, 2021 ). The values of the intra-assay CV ranged from 0.46 to 0.96%, and the values of the inter-assay CV ranged from 1.37 to 1.98%, which indicated that the qPCR method is highly reproducible.…”

Section: Discussionmentioning

confidence: 54%

“…Some farmers used homemade inactivated vaccines (tissue supernatants of diseased mink inactivated using formalin) to prevent the disease and reduce morbidity; however, after a period of application, some minks developed symptoms of Aleutian disease. There are serious safety concerns with this type of vaccine; therefore, it has been discontinued ( Wang et al, 2021 ). In view of this situation, the effective way to prevent the disease is to detect and eliminate the positive animals gradually in China.…”

Section: Discussionmentioning

confidence: 99%

“…MiCV infective rates in minks are high in some fur-bearing animal farms in China ( Cui et al, 2018 ; Wang et al, 2021 ). In this study, we also detected a high positive detection rate of MiCV in minks, with positive detection rates exceeding 55% in Heilongjiang Province, Jilin Province, and Shandong Province.…”

Section: Discussionmentioning

confidence: 99%

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Application of quantitative real-time PCR to detect Mink Circovirus in minks, foxes and raccoon dogs in northern China

Liu,

Sheng,

Zhou

et al. 2023

Front. Microbiol.

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Mink circovirus disease caused by Mink Circovirus (MiCV) is a serious infectious disease of mink that has become prevalent in recent years in China, severely affecting the reproductive performance of mink and causing significant economic losses to farms. To date, there have been few studies on MiCV, its pathogenic mechanism is not clear, and there is no effective vaccine or drug to prevent and control the disease. Therefore, it is necessary to establish a rapid and reliable molecular diagnostic method, which would aid future studies of this novel virus. In our study, we developed a sensitive and specific TaqMan-based quantitative real-time PCR assay targeting the MiCV Cap gene. The assay showed no cross-reaction with other tested animal viruses. The assay is highly sensitive, with a detection limit of as low as 10 plasmid DNA copies and 2.38 × 10−2 pg of viral DNA. The intra and inter--assay coefficients of variation were both low. The positive detection rate of MiCV in clinical samples from minks, foxes, and raccoon dogs were 58.8% (133/226), 50.7% (72/142), and 42.2% (54/128), respectively, giving a total positive detection rate of 52.2% (259/496). Higher contamination levels were observed in samples from the environment in direct or indirect contact with animals, with a total positive detection rate of 75.1% (220/293). These epidemiological results showed that minks, foxes, and raccoon dogs had high infection rates of MiCV. This was also the first study to detect MiCV on the ground and equipment of fur-bearing animal farms. Our assay is highly sensitive and specific for the diagnosis and quantification of MiCV, and should provide a reliable real-time tool for epidemiological and pathogenetic study of MiCV infection.

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Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 99%

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Application of quantitative real-time PCR to detect Mink Circovirus in minks, foxes and raccoon dogs in northern China

Liu,

Sheng,

Zhou

et al. 2023

Front. Microbiol.

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“…Some authors have reported [ 16 ] that in the case of influenza vaccines, employing BEVS instead of the classic embryonated egg platform, the time needed to produce 425 million vials of the vaccine would be 45 days instead of the average six months. FluBlok® [ 17 ] (Protein Sciences Corporation) is a quadrivalent influenza vaccine produced in BEVS by the expression of the hemagglutinin (HA) proteins of four different strains of influenza A and B viruses and is currently FDA-licensed for individuals 18 years old and older.…”

Section: Baculovirus-derived Immunogens For Vaccine Developmentmentioning

confidence: 99%

The Magic Staff: A Comprehensive Overview of Baculovirus-Based Technologies Applied to Human and Animal Health

Pidre

Arrías

Morales

et al. 2022

Viruses

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Baculoviruses are enveloped, insect-specific viruses with large double-stranded DNA genomes. Among all the baculovirus species, Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is the most studied. Due to its characteristics regarding biosafety, narrow host range and the availability of different platforms for modifying its genome, AcMNPV has become a powerful biotechnological tool. In this review, we will address the most widespread technological applications of baculoviruses. We will begin by summarizing their natural cycle both in larvae and in cell culture and how it can be exploited. Secondly, we will explore the different baculovirus-based protein expression systems (BEVS) and their multiple applications in the pharmaceutical and biotechnological industry. We will focus particularly on the production of vaccines, many of which are either currently commercialized or in advanced stages of development (e.g., Novavax, COVID-19 vaccine). In addition, recombinant baculoviruses can be used as efficient gene transduction and protein expression vectors in vertebrate cells (e.g., BacMam). Finally, we will extensively describe various gene therapy strategies based on baculoviruses applied to the treatment of different diseases. The main objective of this work is to provide an extensive up-to-date summary of the different biotechnological applications of baculoviruses, emphasizing the genetic modification strategies used in each field.

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“…The subunit vaccinations have several advantages, including good specificity, reduced side effects, and greater safety. In order to maximize the effectiveness of most subunit vaccinations, immunopotentiators must be added, such as influenza vaccines ( Khalaj-Hedayati et al, 2020 ), COVID-19 vaccines ( Mekonnen et al, 2022 ), and mink virus vaccines ( Wang et al, 2021 ), etc.…”

Section: Introductionmentioning

confidence: 99%

A novel bacterium-like particles platform displaying antigens by new anchoring proteins induces efficacious immune responses

Niu,

Gao,

Ren

et al. 2024

Front. Microbiol.

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Bacterium-like particles (BLP) are the peptidoglycan skeleton particles of lactic acid bacteria, which have high safety, mucosal delivery efficiency, and adjuvant effect. It has been widely used in recent years in the development of vaccines. Existing anchoring proteins for BLP surfaces are few in number, so screening and characterization of new anchoring proteins are necessary. In this research, we created the OACD (C-terminal domain of Escherichia coli outer membrane protein A) to serve as an anchoring protein on the surface of BLP produced by the immunomodulatory bacteria Levilactobacillus brevis 23017. We used red fluorescent protein (RFP) to demonstrate the novel surface display system’s effectiveness, stability, and ability to be adapted to a wide range of lactic acid bacteria. Furthermore, this study employed this surface display method to develop a novel vaccine (called COB17) by using the multi-epitope antigen of Clostridium perfringens as the model antigen. The vaccine can induce more than 50% protection rate against C. perfringens type A challenge in mice immunized with a single dose and has been tested through three routes. The vaccine yields protection rates of 75% for subcutaneous, 50% for intranasal, and 75% for oral immunization. Additionally, it elicits a strong mucosal immune response, markedly increasing levels of specific IgG, high-affinity IgG, specific IgA, and SIgA antibodies. Additionally, we used protein anchors (PA) and OACD simultaneous to show several antigens on the BLP surface. The discovery of novel BLP anchoring proteins may expand the possibilities for creating mucosal immunity subunit vaccines. Additionally, it may work in concert with PA to provide concepts for the creation of multivalent or multiple vaccines that may be used in clinical practice to treat complex illnesses.

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The Mink Circovirus Capsid Subunit Expressed by Recombinant Baculovirus Protects Minks against Refractory Diarrhea in Field

Cited by 3 publications

References 23 publications

Application of quantitative real-time PCR to detect Mink Circovirus in minks, foxes and raccoon dogs in northern China

Application of quantitative real-time PCR to detect Mink Circovirus in minks, foxes and raccoon dogs in northern China

The Magic Staff: A Comprehensive Overview of Baculovirus-Based Technologies Applied to Human and Animal Health

A novel bacterium-like particles platform displaying antigens by new anchoring proteins induces efficacious immune responses

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