The minimizing of fluorescence background in Raman optical activity and Raman spectra of human blood plasma

Tatarkovič, Michal; Synytsya, Alla; Šťovíčková, Lucie; Bunganič, Bohuš; Miškovičová, Michaela; Petruželka, Luboš; Setnička, Vladimı́r

doi:10.1007/s00216-014-8358-7

Cited by 30 publications

(32 citation statements)

References 32 publications

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“…Protocols for the minimization of the fluorescence background, involving the combination of photobleaching and the use of additives as quenchers, are available in literature. [22] In the present case, only minor fluorescence was expected to arise form contaminants of the commercial sample and hence, with Mb being intrinsically fluorescent, the laser treatment (~3-hr photobleaching using 500-600-mW laser power) was the only procedure adopted for fluorescence quenching prior data recording. rRaman spectra of the sample were solvent corrected by subtraction of water and subsequently baseline corrected according to the Eilers-Boelens procedure.…”

Section: Resonance Raman/roamentioning

confidence: 86%

“…Aiming at reducing the undesired fluorescence background causing the saturation of the charge‐coupled device detector, each sample was left in the laser beam for at least 2 hr 30 min, before data recording was initiated. Protocols for the minimization of the fluorescence background, involving the combination of photobleaching and the use of additives as quenchers, are available in literature . In the present case, only minor fluorescence was expected to arise form contaminants of the commercial sample and hence, with Mb being intrinsically fluorescent, the laser treatment (~3‐hr photobleaching using 500–600‐mW laser power) was the only procedure adopted for fluorescence quenching prior data recording.…”

Section: Methodsmentioning

confidence: 98%

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Resonance Raman optical activity of the imidazole–Myoglobin complex: Titrating enhancement

Sgammato

Herrebout

Johannessen

2019

J Raman Spectroscopy

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Resonance Raman optical activity (rROA) is a very powerful chiroptical technique for the investigation of bioactive protein cofactors in native conformation, due to its sensitivity towards any tiny conformational changes occurring at the chromophore. Twelve years ago, the rROA spectrum of myoglobin was published for the first time, revealing the strong potential of the technique for the selective study of the haem moiety. In this contribution, the use of rROA has been extended to the ligand binding properties of myoglobin via a combined approach of resonance Raman/ROA and ultraviolet‐visible absorption/electronic circular dichroism. The treatment of myoglobin with molar excess of imidazole in aqueous solution has led to the formation of a brilliant red pigment, known as imidazolylmet‐myoglobin, and revealed an interesting evolution of the visible Q bands, with the appearance of Qα at 534 nm. The use of the laser excitation of 532 nm, in resonance with the S0–S1 electronic transition (Q bands) of the sample, induced the selective Raman and ROA enhancement of certain haem vibrational modes, in a concentration‐dependent manner. Here, we highlight the sensitivity of rROA spectroscopy towards conformational changes of the haem chromophore upon interaction with the exogenous molecule, allowing us to distinguish between the bound and unligated form of the globin, a fundamental step towards the full understanding of the haem‐ligand binding process.

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Section: Resonance Raman/roamentioning

confidence: 86%

Section: Methodsmentioning

confidence: 98%

Resonance Raman optical activity of the imidazole–Myoglobin complex: Titrating enhancement

Sgammato

Herrebout

Johannessen

2019

J Raman Spectroscopy

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“…There is no obvious visible distortion in the optimized curve either. The prominent Raman peaks of SAM at about 677, 729, 1041, 1324, 1405, 1510, and 2920 cm −1 were assigned to ν (CS), ν s (CN), ω (CH2), ν (CC), ν as (CN), and ν (CH 3 ), respectively [22, 23]. The radius of RCF, as a key parameter, was analyzed to achieve the optimal results.…”

Section: Resultsmentioning

confidence: 99%

Rapid and Quantitative Determination of S-Adenosyl-L-Methionine in the Fermentation Process by Surface-Enhanced Raman Scattering

Ren

Chen

Zhang

et al. 2016

Journal of Analytical Methods in Chemistry

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Concentrations of S-Adenosyl-L-Methionine (SAM) in aqueous solution and fermentation liquids were quantitatively determined by surface-enhanced Raman scattering (SERS) and verified by high-pressure liquid chromatography (HPLC). The Ag nanoparticle/silicon nanowire array substrate was fabricated and employed as an active SERS substrate to indirectly measure the SAM concentration. The linear relationship between the integrated intensity of peak centered at ~2920 cm−1 in SERS spectra and the SAM concentration was established, and the limit of detections of SAM concentrations was analyzed to be ~0.1 g/L. The concentration of SAM in real solution could be predicted by the linear relationship and verified by the HPLC detection method. The relative deviations (δ) of the predicted SAM concentration are less than 13% and the correlation coefficient is 0.9998. Rolling-Circle Filter was utilized to subtract fluorescence background and the optimal results were obtained when the radius of the analyzing circle is 650 cm−1.

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“…[1][2][3][4][5] It has also been used to resolve the structure of several vital biomolecules. [9][10][11][12][13][14][15][16][17] A number of them have successfully used chiroptical spectroscopy of blood plasma, serum, or urine for [This article is part of the Special Issue: Proceedings 16th International Conference on Chiroptical Spectroscopy, Rennes France 2017. However, the vast majority of chiroptical studies have been devoted to single molecules or simple systems, which are not burdened by the complexity of real biofluids.…”

Section: Introductionmentioning

confidence: 99%

“…Some researchers, however, have attempted to overcome issues such as low concentrations of clinically significant molecules, high level of fluorescence, and undesirable interferences disturbing the signals of interest by using chiroptical spectroscopy in biofluid analysis. [9][10][11][12][13][14][15][16][17] A number of them have successfully used chiroptical spectroscopy of blood plasma, serum, or urine for [This article is part of the Special Issue: Proceedings 16th International Conference on Chiroptical Spectroscopy, Rennes France 2017. See the first articles for this special issue previously published in Volume 30:4.…”

Section: Introductionmentioning

confidence: 99%

Electronic circular dichroism for the detection of microalbuminuria

et al. 2018

Self Cite

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Over the past decades, chiroptical spectroscopy has proved its incomparable ability to elucidate the structure and spatial arrangement of chiral molecules. Systematic analysis of biomolecules in the natural environment of biofluids, however, remains challenging. In this study, we used chiroptical spectroscopy to monitor urinary levels of human serum albumin. Not only severe proteinuria but even just a slightly increased urinary excretion of albumin (microalbuminuria) may indicate serious health complications, especially for diabetic individuals. Given the chiral nature of albumin and its typical spectral pattern, it may be easily observable by chiroptical spectroscopy, particularly electronic circular dichroism. The performed chiroptical analysis of urine not only allowed the detection of clinically confirmed microalbuminuria but was also able to reveal this pathological condition in cases beyond the diagnostic capability of common clinical procedures. Thus, our approach suggests that electronic circular dichroism is a useful tool for the fast and reliable qualitative monitoring of microalbuminuria with the potential for a quantitative analysis in the future.

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The minimizing of fluorescence background in Raman optical activity and Raman spectra of human blood plasma

Cited by 30 publications

References 32 publications

Resonance Raman optical activity of the imidazole–Myoglobin complex: Titrating enhancement

Resonance Raman optical activity of the imidazole–Myoglobin complex: Titrating enhancement

Rapid and Quantitative Determination of S-Adenosyl-L-Methionine in the Fermentation Process by Surface-Enhanced Raman Scattering

Electronic circular dichroism for the detection of microalbuminuria

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