The migratory activity of rat epidermal cells in vitro—Age-related changes and the effect of serum

Ooka, Hiroshi; Yamamoto, Kiyotaka; Okuma, Youko; Suga, Sachiko; Wakasugi, Mari

doi:10.1016/0531-5565(75)90017-0

Cited by 6 publications

(1 citation statement)

References 9 publications

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“…Although other attempts have been made to isolate and identify the activity in serum supporting epithelial cell spreading (2,3,5,7,8,27), earlier studies have not characterized that activity sufficiently for a meaningful comparison. It appears the factor described here is different from others in its cofactor dependence.…”

Section: Resultsmentioning

confidence: 99%

Epibolin: a protein of human plasma that supports epithelial cell movement.

Stenn¹

1981

Proc. Natl. Acad. Sci. U.S.A.

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Earlier studies suggested that there is a specific activity in mammalian serum and plasma that supports epidermal (epithelial) cell movement. This activity was shown to be nondialyzable and heat labile. In the studies reported here, using standard biochemical procedures-i.e., ammonium sulfate fractionation, ion-exchange and gel filtration chromatography, isoelectric precipitation, and preparative polyacrylamide gel electrophoresis-we have purified a factor from human plasma that supports epidermal cell movement. The factor travels as an apparent single band on disc gel electrophoresis and corresponds to a glycosylated single-chain protein ofapproximately 65,000 ± 3,000 daltons. The purified fraction is necessary and sufficient for epithelial cell movement in three in vitro assays: (i) spreading of dissociated epidermal cells, (ii) outgrowth ofepithelial sheets from skdn explants, and (iii) epiboly, epithelial sheet movement over a floating skin explant. The purified fraction is active at a concentration of 1-2 pg/ml ofgrowth medium. It is destroyed by trypsin and its activity is augmented more than 10-fold by a second, as yet unpurified, fraction of plasma. These studies support the notion that a single protein ofplasma supports epidermal cell movement and that this protein may play an important role in wound closure. Because it supports epiboly, the most biologically relevant ofthe assays, it has been named epibolin

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