Serum epidermal migration-stimulating factor?Membrane ultrafiltration and physical stability characteristics

Fixation and staining procedures were developed for the electron microscopic demonstration of glycosaminoglycans (GAGs) in human epidermis. En bloc staining with cuprolinic blue (CB), ruthenium red (RR) and tannic acid (TA) in the primary fixative were applied for the localization of the GAGs. Removal of the epidermal basal lamina and underlying dermis was a prerequisite for stain penetration. In CB-fixed specimens 50 nm long, rod-like granules were found attached to keratinocyte cell surfaces, while the RR- and TA-fixed specimens contained round granules (luminal diameter 10 and 30 nm, respectively). The stainability of the CB-positive granules in the presence of 0.3 mol/l MgCl2 indicated that they contained sulphated GAGs. Prefixation digestions of epidermal sheets with chondroitinase ABC. Streptomyces hyaluronidase, and heparitinase showed that the RR-positive granules also contained sulphated GAGs, mostly heparan sulphate. The granules visualized with TA on keratinocytes were susceptible to heparitinase treatment, but the abundance of TA-staining suggested that TA also stained structures other than heparan sulphate. The EM data was in accordance with the 35SO4 labelling experiments showing that heparan sulphate was the major sulphated GAG synthesized in epidermis, whereas chondroitin/dermatan sulphates comprised about one fifth of the total activity incorporated. The distributions of the CB-, RR- and TA-positive granules on cell surfaces were similar. The morphology of the proteoglycan granules was probably determined by the extent of the GAG-chain collapse following binding to each of the dyes.

show abstract

Ultrastructural localization of keratinocyte surface associated heparan sulphate proteoglycans in human epidermis

Tammi

Hyyryläinen

Maibach

et al. 1987

Histochemistry

View full text Add to dashboard Cite

show abstract

A model system for long-term serum-free suspension organ culture of human fetal tissues: experiments on digits and skin from multiple body regions

Zeltinger

Holbrook

1997

Cell Tissue Res

View full text Add to dashboard Cite

We have optimized a suspension organ culture (SOC) system previously used to study human fetal skin in vitro. Eleven types of media were used to culture fetal digits and skin samples (ranging from 59-91 days of estimated gestational age) for periods of 3 or 4 weeks. The cultures were supplemented with 0%-10% bovine calf serum and exposed to 5%-45% oxygen atmospheres. Optimal conditions, which were selected based upon the morphologic and histologic properties of the cultured tissues, were: DMEM-F12 medium in a serum-free environment with a 45% oxygen atmosphere. The refined SOC system supports the maintenance and/or continued development of the epidermis and dermis of trunk, back, scalp, and digital skin, developing hair follicles, nails, and glands. Overall, development and differentiation mimicked the in vivo state more closely than that observed when using other in vitro culture systems. The effects of bovine calf serum and oxygen levels are presented, and the potential for manipulating the environment of this system for studying mechanisms of cutaneous development is also discussed.

show abstract

Influence of Human Adipose-Derived Stromal Cells on Wnt Signaling in Organotypic Skin Culture

Kim

Lee²,

Yoon³

et al. 2011

Journal of Craniofacial Surgery

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Serum epidermal migration-stimulating factor?Membrane ultrafiltration and physical stability characteristics

Cited by 4 publications

References 13 publications

Ultrastructural localization of keratinocyte surface associated heparan sulphate proteoglycans in human epidermis

Ultrastructural localization of keratinocyte surface associated heparan sulphate proteoglycans in human epidermis

A model system for long-term serum-free suspension organ culture of human fetal tissues: experiments on digits and skin from multiple body regions

Influence of Human Adipose-Derived Stromal Cells on Wnt Signaling in Organotypic Skin Culture

Contact Info

Product

Resources

About