Influence of Human Adipose-Derived Stromal Cells on Wnt Signaling in Organotypic Skin Culture

Kim, Deok Woo; Lee, Jae Sun; Yoon, Eul Sik; Lee, Byung Il; Park, Seung Ha; Dhong, Eun Sang

doi:10.1097/scs.0b013e3182077fa2

Cited by 8 publications

(4 citation statements)

References 31 publications

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“…Notch signaling is involved in the cross-talk with other pathways particularly Wnt signaling not only in hematopoiesis [147] but also in other cellular development [136, 148–151]. In addition, Wnt signaling pathway is mediated in the regulation of stem cell fate and maintenance of mouse ESCs and human ESCs in undifferentiated state [152, 153].…”

Section: Signaling Pathways In Self-renewal and Maintenance Of Hscsmentioning

confidence: 99%

Hematopoietic Stem Cell Development, Niches, and Signaling Pathways

Chotinantakul

Leeanansaksiri

2012

Bone Marrow Research

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Hematopoietic stem cells (HSCs) play a key role in hematopoietic system that functions mainly in homeostasis and immune response. HSCs transplantation has been applied for the treatment of several diseases. However, HSCs persist in the small quantity within the body, mostly in the quiescent state. Understanding the basic knowledge of HSCs is useful for stem cell biology research and therapeutic medicine development. Thus, this paper emphasizes on HSC origin, source, development, the niche, and signaling pathways which support HSC maintenance and balance between self-renewal and proliferation which will be useful for the advancement of HSC expansion and transplantation in the future.

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Section: Signaling Pathways In Self-renewal and Maintenance Of Hscsmentioning

confidence: 99%

Hematopoietic Stem Cell Development, Niches, and Signaling Pathways

Chotinantakul

Leeanansaksiri

2012

Bone Marrow Research

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“…Human adipose-derived stromal cells have been shown to produce cytokines in a paracrine manner. These induce the expression of Wnt1 in the de-epithelialized skin, mainly in the dermal fibroblasts [53]. These data suggest that Wnt signaling plays a role in skin regeneration and wound closure.…”

Section: Discussionmentioning

confidence: 77%

Enhancement of wound closure in diabetic mice by ex vivo expanded cord blood CD34+ cells

Chotinantakul¹,

Dechsukhum²,

Dejjuy³

et al. 2013

Cellular and Molecular Biology Letters

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Diabetes can impair wound closure, which can give rise to major clinical problems. Most treatments for wound repair in diabetes remain ineffective. This study aimed to investigate the influence on wound closure of treatments using expanded human cord blood CD34 + cells (CB-CD34 + cells), freshly isolated CB-CD34 + cells and a cytokine cocktail. The test subjects were mice with streptozotocin-induced diabetes. Wounds treated with fresh CB-CD34 + cells showed more rapid repair than mice given the PBS control. Injection of expanded CB-CD34 + cells improved wound closure significantly, whereas the injection of the cytokine cocktail alone did not improve wound repair. The results also demonstrated a significant decrease in epithelial gaps and advanced re-epithelialization over the wound bed area after treatment with either expanded CB-CD34 + cells or freshly isolated cells compared with the control. In addition, treatments with both CB-CD34 + cells and the cytokine cocktail were shown to promote recruitment of CD31 + -endothelial cells in the wounds. Both the CB-CD34 + cell population and the cytokine treatments also enhanced the recruitment of CD68-positive cells in the early stages (day 3) of treatment compared with PBS control, although the degree of this enhancement was found to decline in the later stages (day 9). These results demonstrated that expanded CB-CD34 + cells or freshly isolated CB-CD34 + cells could accelerate wound repair by increasing the recruitment of macrophages and capillaries and the reepithelialization over the wound bed area. Our data suggest an effective role in wound closure for both ex vivo expanded CB-CD34 + cells and freshly isolated cells, and these may serve as therapeutic options for wound treatment for diabetic patients. Wound closure acceleration by expanded CB-CD34 + cells also breaks the insufficient quantity obstacle of stem cells per unit of cord blood and other stem cell sources, which indicates a broader potential for autologous transplantation.

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“…6,26 In our recent study, we could find that hASCs promoted canonical Wnt signaling in the dermis of skin in culture through paracrine effects. 9 This may account for the antiaging effects of the hASCs on skin, as these cells have been shown to stimulate dermal fibroblast proliferation. 27 In our previous study, we focused on the dermal fibroblast and were not able to assess epidermal biology.…”

Section: Discussionmentioning

confidence: 99%

“…7,8 In our recent study, we determined that hASCs promote dermal canonical Wnt signaling in organotypic skin culture through paracrine mediators expressed by these cells in culture. 9 However, direct histological evidence of these beneficial effects was not thoroughly established, and the safety of clinical application remains to be established.…”

mentioning

confidence: 98%

Morphologic Changes in Photodamaged Organotypic Human Skin Culture After Treatment of Autologous Adipose-Derived Stromal Cells

Dhong¹,

Hwang²,

Kim³

et al. 2012

Journal of Craniofacial Surgery

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Influence of Human Adipose-Derived Stromal Cells on Wnt Signaling in Organotypic Skin Culture

Abstract: The results of the current study showed that hASCs promoted canonical Wnt signaling in organotypic skin culture through paracrine effects, and the increased Wnt signaling was mainly due to dermal fibroblasts.

Cited by 8 publications

References 31 publications

Hematopoietic Stem Cell Development, Niches, and Signaling Pathways

Hematopoietic Stem Cell Development, Niches, and Signaling Pathways

Enhancement of wound closure in diabetic mice by ex vivo expanded cord blood CD34+ cells

Morphologic Changes in Photodamaged Organotypic Human Skin Culture After Treatment of Autologous Adipose-Derived Stromal Cells

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