The migration speed of nucleolar precursor bodies in pronuclei affects in vitro fertilization‐derived human embryo ploidy status and live birth

Inoue, Taketo; Taguchi, Sayumi; Uemura, Mikiko; Tsujimoto, Yoshiko; Kokunai, Kana; Ikawa, Kayoe; Yamashita, Yoshiki

doi:10.1002/rmb2.12497

Cited by 3 publications

(2 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The blastocysts were cryopreserved once. The PGT‐A protocol has been described previously 28 . Euploidy was defined as mosaicism with <30% of the biopsied cells.…”

Section: Methodsmentioning

confidence: 99%

Therapeutic efficacy of the optimization of thyroid function, thrombophilia, immunity and uterine milieu (OPTIMUM) treatment strategy on pregnancy outcomes after single euploid blastocyst transfer in advanced age women with recurrent reproductive failure

Kuroda,

Horikawa,

Moriyama

et al. 2023

Reprod Medicine & Biology

View full text Add to dashboard Cite

PurposeTo clarify the efficacy of the OPtimization of Thyroid function, Thrombophilia, IMmunity and Uterine Milieu (OPTIMUM) treatment strategy on pregnancy outcomes after euploid blastocyst transfer in advanced age women with recurrent implantation failure (RIF) and/or recurrent pregnancy loss (RPL).MethodsBetween January 2019 and May 2022, 193 consecutive women aged ≥40 years with RIF and/or RPL received single euploid blastocyst transfer. Before embryo transfer, 127 women underwent RIF/RPL testing. Chronic endometritis was treated with mainly antibiotics, aberrant high Th1/Th2 cell ratios with vitamin D and/or tacrolimus, overt/subclinical hypothyroidism with levothyroxine, and thrombophilia with low‐dose aspirin. We compared pregnancy outcomes in the women who did and did not receive the OPTIMUM treatment strategy.ResultsWomen with RIF/RPL in the OPTIMUM group had significantly higher clinical pregnancy and livebirth rates than did those in the control group (clinical pregnancy rate of 71.7% and 45.5%, p < 0.001; livebirth rate of 64.6% and 39.4%, p = 0.001, respectively). However, preimplantation genetic testing for aneuploidy with and without OPTIMUM promoted low miscarriage rates with no significant difference between them (9.9%, and 13.3%, respectively; p = 0.73).ConclusionsThe OPTIMUM treatment strategy improved clinical pregnancy rates after single euploid blastocyst transfer; but not miscarriage rates.

show abstract

“…The blastocysts were cryopreserved once. The PGT‐A protocol has been described previously 28 . Euploidy was defined as mosaicism with <30% of the biopsied cells.…”

Section: Methodsmentioning

confidence: 99%

Therapeutic efficacy of the optimization of thyroid function, thrombophilia, immunity and uterine milieu (OPTIMUM) treatment strategy on pregnancy outcomes after single euploid blastocyst transfer in advanced age women with recurrent reproductive failure

Kuroda,

Horikawa,

Moriyama

et al. 2023

Reprod Medicine & Biology

View full text Add to dashboard Cite

show abstract

“…It is positively associated with the rates of euploid blastocysts and live birth, with a cut‐off value of approximately 3.7–4.6 μm/h. 42 , 43 Measuring the speed by specific software may serve as a predictor of embryonic and pregnancy outcomes.…”

Section: Fertilization Stagementioning

confidence: 99%

Developmental perturbation in human embryos: Clinical and biological significance learned from time‐lapse images

Ezoe,

Takahashi,

Miki

et al. 2024

Reprod Medicine & Biology

View full text Add to dashboard Cite

BackgroundTime‐lapse technology (TLT) has gained widespread adoption worldwide. In addition to facilitating the undisturbed culture of embryos, TLT offers the unique capability of continuously monitoring embryos to detect spatiotemporal changes. Although these observed phenomena play a role in optimal embryo selection/deselection, the clinical advantages of introducing TLT remain unclear. However, manual annotation of embryo perturbation could facilitate a comprehensive assessment of developmental competence. This process requires a thorough understanding of embryo observation and the biological significance associated with developmental dogma and variation. This review elucidates the typical behavior and variation of each phenomenon, exploring their clinical significance and research perspectives.MethodsThe MEDLINE database was searched using PubMed for peer‐reviewed English‐language original articles concerning human embryo development.Main findingsTLT allows the observation of consecutive changes in embryo morphology, serving as potential biomarkers for embryo assessment. In assisted reproductive technology laboratories, several phenomena have not revealed their mechanism, posing difficulties such as fertilization deficiency and morula arrest.ConclusionA profound understanding of the biological mechanisms and significance of each phenomenon is crucial. Further collaborative efforts between the clinical and molecular fields following translational studies are required to advance embryonic outcomes and assessment.

show abstract

Zygotic spindle orientation defines cleavage pattern and nuclear status of human embryos

Porokh,

Kyjovská,

Martonová

et al. 2024

Nat Commun

View full text Add to dashboard Cite

The first embryonic division represents a starting point for the development of a new individual. In many species, tight control over the first embryonic division ensures its accuracy. However, the first division in humans is often erroneous and can impair embryo development. To delineate the spatiotemporal organization of the first mitotic division typical for normal human embryo development, we systematically analyzed a unique timelapse dataset of 300 IVF embryos that developed into healthy newborns. The zygotic division pattern of these best-quality embryos was compared to their siblings that failed to implant or arrested during cleavage stage. We show that division at the right angle to the juxtaposed pronuclei is preferential and supports faithful zygotic division. Alternative configurations of the first mitosis are associated with reduced clustering of nucleoli and multinucleation at the 2-cell stage, which are more common in women of advanced age. Collectively, these data imply that orientation of the first division predisposes human embryos to genetic (in)stability and may contribute to aneuploidy and age-related infertility.

show abstract

The migration speed of nucleolar precursor bodies in pronuclei affects in vitro fertilization‐derived human embryo ploidy status and live birth

Cited by 3 publications

References 52 publications

Therapeutic efficacy of the optimization of thyroid function, thrombophilia, immunity and uterine milieu (OPTIMUM) treatment strategy on pregnancy outcomes after single euploid blastocyst transfer in advanced age women with recurrent reproductive failure

Therapeutic efficacy of the optimization of thyroid function, thrombophilia, immunity and uterine milieu (OPTIMUM) treatment strategy on pregnancy outcomes after single euploid blastocyst transfer in advanced age women with recurrent reproductive failure

Developmental perturbation in human embryos: Clinical and biological significance learned from time‐lapse images

Zygotic spindle orientation defines cleavage pattern and nuclear status of human embryos

Contact Info

Product

Resources

About